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Citations for NanoTemper :
1 - 50 of 159 citations for Cow Ataxin 10 ATXN10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001, NanoTemper technologies, Germany). The concentration of labeled PfCoSP protein was kept constant at a concentration of 90 nM ...
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bioRxiv - Biochemistry 2021Quote: ... Ammonium sulfate suspension of the MDH was centrifuged at 23,000 × g for 1 min at 4 °C and the precipitate was resuspended in the labeling buffer to gain 90 μL of 10 μM MDH and mixed with 10 μL of 300 μM of dye (NanoTemper Technologies) prepared in dimethyl sulfoxide to create 100 μl of dye-protein solution ...
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bioRxiv - Biophysics 2022Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
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bioRxiv - Biophysics 2021Quote: ... 10 μL samples were loaded in capillaries (TY-C001, Nanotemper). The intrinsic protein fluorescence was measured at 350 nm and 330 nm ...
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bioRxiv - Cell Biology 2022Quote: ... employing the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper). The labeled protein was dissolved in a buffer containing PBS ...
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bioRxiv - Biophysics 2023Quote: ... 10 µL samples were aspirated in capillaries (TY-C001, Nanotemper, Germany), and intrinsic fluorescence was measured at 350 nm and 330 nm while the temperature was ramped from 35 °C to 95 °C at a rate of 30 °C/min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10 ul was sampled in a Monolith.NT115 capillary (NanoTemper MO-K022) and measurements were performed at 25°C using standard parameters ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...
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DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... 10 μl were loaded onto the Monolith NT.115 Premium Capillaries (NanoTemper). The MST signal was recorded with NT.Control v2.1.31 software and data was fitted to a Kd model with MO.Affinity Analysis v2.3 software.
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... 10 mM NaCl and transferred to capillaries (Standard treated, NanoTemper Technologies GmbH, Germany), measurements were performed at 37 °C with a constant blue LED power of 20% and at MST infrared laser powers of 40 % to induce thermophoretic motion ...
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bioRxiv - Biophysics 2021Quote: ... 10 μl samples were manually loaded into nanoDSF Grade Standard Capillaries (NanoTemper Technologies) and transferred to a Prometheus NT.48 nanoDSF device (NanoTemper Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
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bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
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bioRxiv - Biochemistry 2019Quote: MST was measured on 10 μl reactions using standard capillaries (MO-K022, NanoTemper Technologies) on a Monolith NT.115 (NanoTemper Technologies ...
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bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
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bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
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bioRxiv - Microbiology 2024Quote: ... and the NHS-red-tag kit (Nanotemper, Germany) was used to label ConA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... pH 8.2) was mixed with 10 μL dye (Dye RED-NHS 2nd Generation (Nanotemper MO-L011)) solution (300 μM ...
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bioRxiv - Immunology 2022Quote: ... and ∼10 µl of each diluted sample (in duplicates) was loaded into NanoDSF capillaries (NanoTemper Technologies). The temperature was raised from 20°C to 95°C at 1°C/min rate ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 10 μL of each diluted sample was loaded into nanoDSF grade high-sensitivity capillaries (NanoTemper) in triplicate and placed on the instrument’s capillary loading tray ...
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bioRxiv - Microbiology 2020Quote: ... Each protein (10 μM) was labeled with NT-647-NHS reactive dye (30 μM) (Nanotemper, Germany), which reacted efficiently with the primary amines of the proteins to form a stable dye protein conjugate ...
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bioRxiv - Biophysics 2020Quote: ... pH 8.2) was mixed with 10 µl of 300 µM NT647-N-hydroxysuccinimide fluorophore (NanoTemper Technologies) and incubated in the dark for 30 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: The BD5.1 protein was labelled with the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper Technologies, Catalog# MO-L018) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... 10 μl of 100 nM labeled NHR2 in MST-buffer (included with the capillary set from NanoTemper) was mixed with 10 μl unlabeled NHR2 with concentrations ranging from 10.3 nM to 337.5 μM ...
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bioRxiv - Microbiology 2020Quote: ... Monolith His-Tag Labeling kit (MO-L018) was from NanoTemper Technologies.
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bioRxiv - Biochemistry 2021Quote: ... Thermophoresis data were analyzed when fluorescence signals lies within the 10% acceptance range using MO Affinity Analysis software V2.1.2 (Nanotemper). Data were plotted using GraphPad Prism 4.0 software and fitted with nonlinear equation.
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bioRxiv - Molecular Biology 2023Quote: ... The mixtures were incubated at room temperature for 10 min and transferred to 16 standard capillaries (Nanotemper, Germany). Data were acquired using medium MST power ...
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bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
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bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
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bioRxiv - Molecular Biology 2020Quote: ... 10-µL of undiluted rAAV of each lot was loaded into a high sensitivity capillary (Nanotemper Cat#PR-C006) and run on a Nanotemper Prometheus NT.48 nanoDSF instrument ...
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bioRxiv - Biochemistry 2019Quote: ... 10 μL of the different dilutions of samples were filled into hydrophilic treated capillaries (premium coated capillaries, NanoTemper Technologies) and measured after a 10 min equilibration at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... LLPS) were prepared in 1.5 ml Eppendorf tubes and loaded into 10 μl high sensitivity microcapillaries (NanoTemper Technologies, Germany) and subjected to DLS measurements ...
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bioRxiv - Molecular Biology 2023Quote: ... a volume of 10 μl was then placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
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bioRxiv - Biochemistry 2023Quote: ... Approximately 10 µL of each sample was loaded into Prometheus NT.48 Series nanoDSF high sensitivity capillaries (NanoTemper, Germany) and melting scans were performed using the Pr.ThermControl software (NanoTemper ...
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bioRxiv - Bioengineering 2023Quote: ... The samples (10 µL at 0.2 mg/mL in HBS) were loaded into standard treated NT.115 capillaries (NanoTemper), and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper ...
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bioRxiv - Cancer Biology 2023Quote: ... Aliquots (10 μl) of purified mTORC1_WT or mTORC1_EWEDdup variant were loaded into the Prometheus capillaries (Cat. PR-C002, NanoTemper Technologies), and the fluorescence intensity at 330 nm and 350 nm was recorded as a function of temperature from 10 °C to 90 °C ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
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bioRxiv - Biophysics 2021Quote: ... or Monolith NT™ (Protein Labeling Kit Blue-NHS, NanoTemper GmbH, Germany). The labeling was achieved by incubating 115 μM of protein and 350 μM of dye (465 μl reaction volume ...
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bioRxiv - Cell Biology 2023Quote: His-Tag labeling Kit RED-tris-NTA 2nd generation (MO-L018, Nanotemper) was used to label His-tagged Bax purchased from Creative BioMart (Catalog No ...
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bioRxiv - Biochemistry 2023Quote: ... using a His-tag Labeling Kit (NanoTemper Technologies, cat. no. MO-L018). For measurements using CBP30 ...
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bioRxiv - Biochemistry 2023Quote: ... Monolith protein labeling kit RED-NHS 2nd generation was purchased from NanoTemper Technologies (Munich ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the proteins were labeled with a RED-NHS Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... were done in the commercial MST buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2; Nanotemper Technologies) supplied with 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2021Quote: ... the mixtures were left to incubate for 10 min at room temperature and subsequently loaded in premium coated capillary tubes (NanoTemper). Measurements were performed with a Monolith NT.115 (NanoTemper ...
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bioRxiv - Molecular Biology 2023Quote: ... pH6) in a volume of 10 μl and placed in Prometheus Series High Sensitivity Capillaries (NanoTemper, Cat. no. PR-C006).
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were loaded into the MonoLith NT.115 standard treated capillaries and incubated for 10 min before analysis using the Monolith NT.115 instrument (NanoTemper Technologies) at 21°C using medium laser power and 40 % LED power ...
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bioRxiv - Cell Biology 2021Quote: ... proteins at 200 nM were incubated with varying concentrations of CaCl2 and ZnSO4 in a 10 µl mixture and loaded into NT.115 standard coated capillaries (NanoTemper Technologies). The buffers (25 mM HEPES ...