Labshake search
Citations for NanoTemper :
1 - 50 of 60 citations for Boc 6 fluoro DL tryptophan since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: EsxA sample purity was confirmed using tryptophan fluorescence (NanoTemper Tycho). Pure MBP was used as a negative control (sample impurity control ...
-
bioRxiv - Cell Biology 2021Quote: DLS measurements were done using the Prometheus Panta (Nanotemper, Munich, Germany). For DLS measurements with good signal-to-noise ...
-
bioRxiv - Biophysics 2024Quote: The melting temperatures (Tm) of recombinant VDAC were determined by native tryptophan fluorescence using a Tycho Differential Scanning Fluorometer (Nanotemper). VDAC samples were diluted to 1mg/ml in 25 mM HEPES at pH 7.4 ...
-
bioRxiv - Biophysics 2024Quote: DLS studies of N-arm peptides were carried out in a Prometheus Panta (Nanotemper, Germany) instrument at 20°C ...
-
bioRxiv - Biochemistry 2020Quote: Protein melting temperatures were determined by difference scanning fluorimetry utilizing the intrinsic fluorescence of tryptophan and tyrosine measured in a Tycho NT6 System (NanoTemper, Munich, Germany). Results are average of three independent measurements with two technical replicates for each measurement.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The thermal denaturation curves were measured by using intrinsic tryptophan fluorescence of NHR2 (with and without compound) on nanoDSF (NanoTemper, Prometheus NT.40, Germany). The temperature was increased from 20 to 95 °C at a rate of 1 °C/min ...
-
bioRxiv - Biophysics 2022Quote: Measurements were carried out using a high sensitivity and high throughput capillary based DLS instrument—Prometheus PANTA (NanoTemper Technologies, Germany). α-Syn solutions (monomer ...
-
bioRxiv - Plant Biology 2021Quote: ... 6 (NanoTemper Technologies). Assays were done at 5.8 μM protein in 0.2 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biophysics 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
-
bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
-
bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Pre-dialyzed protein and mutant samples were diluted (∼0.5 mg/mL ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6 (NanoTemper Technologies). Spike ectodomains were diluted to approximately 0.15LJmg/mL ...
-
The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... 6 (NanoTemper Technologies, Germany). The measurements were performed at 0.1 mg/ml protein concentration in 15 mM Tris-SO4 pH 7.5 ...
-
bioRxiv - Microbiology 2024Quote: ... using the Tycho NT.6 (NanoTemper). DSF experiments were set up to a final volume of 10 µl of buffer C containing the EccC5DUF construct at 2 µM in the absence or in the presence of ADP-AlF3 at 4 mM ...
-
bioRxiv - Biochemistry 2020Quote: ... using a Tycho NT.6 instrument (Nanotemper), which allowed for determination of NT-mini’s unfolding temperatures in the different assayed conditions ...
-
bioRxiv - Cell Biology 2024Quote: A Tycho NT.6 instrument (Nanotemper Technologies) was used to determine the melting stability of human PLA2G15-6xHis wildtype and S198A ...
-
bioRxiv - Biochemistry 2023Quote: We used the Tycho NT.6 (NanoTemper Technologies) for label-free thermal shift analysis ...
-
bioRxiv - Biochemistry 2021Quote: ... and RBD-71 measured using the Tycho NT.6 (NanoTemper).
-
bioRxiv - Biochemistry 2024Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
-
bioRxiv - Biochemistry 2020Quote: His6-GlnA1 was analyzed using Tycho NT.6 (NanoTemper, Munich, Germany) by applying a standard capillary (10 µl ...
-
bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
-
bioRxiv - Biochemistry 2024Quote: The thermal stability was performed using Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and kifunensine-treated SIDT1ECD were diluted to a final concentration of 0.25 mg/mL at various pH (pH 7.5 ...
-
bioRxiv - Immunology 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15LmgLml−1 ...
-
bioRxiv - Biophysics 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15 mg ml−1 ...
-
bioRxiv - Biochemistry 2022Quote: ... Data were acquired with a Tycho NT.6 equipment (NanoTemper Technologies). The emission fluorescence intensity was recorded at 330 nm and 35 ºC ...
-
bioRxiv - Biophysics 2024Quote: ... The thermostability assay was performed using Tycho NT.6 (NanoTemper Technologies). Protein samples were heated from 35 °C to 95 °C at 30 °C per minute ...
-
bioRxiv - Molecular Biology 2022Quote: ... We used Tycho analysis (Tycho NT.6, NanoTemper Technologies GmbH, Munich, Germany) to follow the interaction between VcDnaB or EcDnaB and VcDciA ...
-
A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
-
bioRxiv - Microbiology 2024Quote: The thermal shift assay was conducted using a Tycho NT.6 (NanoTemper) system ...
-
bioRxiv - Biochemistry 2023Quote: The thermal stability analysis was conducted using the Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and SIDT2ECD were diluted to a final concentration of 0.25 mg/mL at different pH values (pH 7.5 ...
-
bioRxiv - Microbiology 2024Quote: The protein thermal stability measurements were performed using Tycho NT.6 (NanoTemper Technologies). The purified protein samples were diluted in the test condition buffers to a range of concentrations from 1 to 2 mg mL-1 ...
-
bioRxiv - Biochemistry 2024Quote: ... pH 8.0 were performed using a Tycho NT.6 instrument (NanoTemper Technologies GmbH). Data were analyzed and plotted using Graphpad Prism 760.
-
bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
-
bioRxiv - Biophysics 2023Quote: ... and experiments were carried out using the NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany). The temperature gradient was set to an increase of 30 °C/min from 35° C to 95° C ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
-
bioRxiv - Pathology 2024Quote: ... pH 7.6) and the thermal stability was measured on a Tycho NT.6 (NanoTemper Technologies) between 35 and 95 °C ...
-
bioRxiv - Cancer Biology 2023Quote: Label-free thermal shift assay experiments were performed with a Tycho NT.6 instrument (NanoTemper Technologies) based on a reported protocol 76 ...
-
bioRxiv - Immunology 2024Quote: Stability of FL S(P2) proteins was measured by Tycho NT.6 (NanoTemper, firmware version: 1.10.3) as described previously 23 ...
-
bioRxiv - Molecular Biology 2021Quote: Thermal stability was evaluated by nanoDSF using the Tycho NT.6 system (NanoTemper Technologies GmbH, München, Germany) 68 ...
-
bioRxiv - Biophysics 2020Quote: Samples thermostability was measured by nano differential scanning fluorimetry using a Tycho NT.6 instrument (NanoTemper Technologies) that measures the intrinsic fluorescence from tryptophan and tyrosine residues detected at emission wavelengths of 350 and 330 nm ...
-
bioRxiv - Plant Biology 2021Quote: ... 0.5 mM NaCl and run in duplicates in Tycho NT.6 capillaries (NanoTemper Technologies, Cat# TY-C001). Different protein solutions were prepared to reach a final CaCl2 concentrations of 0.01 mM ...
-
Defining short linear motif binding determinants by phage-based multiplexed deep mutational scanningbioRxiv - Biochemistry 2024Quote: ... Protein purity and quality was confirmed through SDS-PAGE and thermal shift assay (Tycho NT.6, NanoTemper). His-GST tagged domains of SARS-CoV-2 proteins were expressed and harvested as described above ...
-
bioRxiv - Bioengineering 2024Quote: The apparent melting temperature of the three nanobodies that expressed was measured with a Tycho NT.6 (Nanotemper). Intrinsic fluorescence of tryptophan and tyrosine residues was recorded at 330 and 350 nm with a fixed 30 °C/min temperature ramp from 35 to 95 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... tuberculosis DnaE1 was determined in the absence and presence of the ligand 6-thioinosine using a Tycho instrument (Nanotemper). 8 μM of M ...
-
bioRxiv - Microbiology 2021Quote: The thermal stability of wild-type HigBHigA and HigB(L5ext)HigA were assessed using a Tycho NT.6 instrument (NanoTemper). Protein was heated at 0.1°C steps over a temperature range of 35°C to 95°C ...
-
bioRxiv - Biochemistry 2020Quote: DSF assays were performed on LH3 wild-type and mutants using a Tycho NT.6 instrument (NanoTemper Technologies GmbH, Germany). LH3 samples at a concentration of 1 mg/mL in a buffer composed of 25 mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: ... TmrAB was diluted to 8.5 μM in SEC buffer and prepared in duplicate for measurement on a Tycho NT.6 (NanoTemper Technologies). Data are reported as the ratio of fluorescence intensity at 350 nm and 330 nm normalized to the minimum and maximum values of the whole dataset.
-
bioRxiv - Microbiology 2021Quote: ... Quality control during protein purification was carried out using reducing and non-reducing SDS-PAGE analysis and differential scanning fluorimetry (DSF) with a Tycho NT.6 instrument (Nanotemper). All samples were concentrated to 1 mg mL-1 ...
-
bioRxiv - Biochemistry 2022Quote: ... NCS-1ΔH10/Ric-8A-P2 peptide and NCS-1ΔH10/Ric-8A-P3 peptide were performed using a Tycho NT.6 instrument (NanoTemper Technologies). Proteins at 10 μM in their corresponding final buffers (see above ...
-
bioRxiv - Microbiology 2024Quote: ... The folded state and stability of the protein was assessed by SEC using a calibrated HiLoad S200 16/600 column and thermal calorimetry using the Tycho NT.6 system (NanoTemper).