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Citations for NanoTemper :
1 - 50 of 83 citations for 6 Oxabicyclo 3.1.0 hexan 2 ol 4 tetrahydro 2H pyran 2 yl oxy 1R 1 α 2 bta 4 α 5 α 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
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bioRxiv - Cell Biology 2021Quote: ... Samples in the presence and absence of 2 mM ATP with 2 mM MgCl2 were loaded in high-sensitivity capillaries (Nanotemper, Munich, Germany) and heated with a 0.3°C/min temperature ramp from 20°C to 70°C with a 2 sec DLS integration time.
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bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Biophysics 2021Quote: The data was analyzed and the Ktet (Eq. 1) or EC50 (Eq. 2) calculated using the MO affinity analysis software (NanoTemper, Germany), considering a 1:1 model.
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bioRxiv - Pharmacology and Toxicology 2020Quote: The thermostability of MAPK14 was measured in the presence of different concentrations of MBZ in 50 mM HEPES buffer in the presence of 200 mM NaCl and 2% DMSO at pH 7.5 using a label-free fluorimetric analysis with a Prometheus NT.Plex instrument (NanoTemper Technologies), as described previously [52] ...
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bioRxiv - Biochemistry 2023Quote: 300 nM 6His-tagged EF-Tu was incubated with 75 nM Red Tris NTA dye Generation 2 (NanoTemper Technologies) in binding buffer (287 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...
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bioRxiv - Biochemistry 2023Quote: ... was measured from 15°C to 95°C with a ramp rate of 2°C per minute with a Prometheus NT.48 nano-fluorimeter (NanoTemper). The melting temperatures (Tm values ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein (Pry1 or Na-ASP-2) was fluorescently labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labeled protein (Pry1 or Na-ASP-2 ...
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bioRxiv - Molecular Biology 2020Quote: ... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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bioRxiv - Plant Biology 2021Quote: ... 6 (NanoTemper Technologies). Assays were done at 5.8 μM protein in 0.2 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Pre-dialyzed protein and mutant samples were diluted (∼0.5 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 (NanoTemper Technologies). Spike ectodomains were diluted to approximately 0.15LJmg/mL ...
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... 6 (NanoTemper Technologies, Germany). The measurements were performed at 0.1 mg/ml protein concentration in 15 mM Tris-SO4 pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The labeled protein was 1:1 incubated with a series dilution of ABT199 (4 uM to 0.122 nM) to perform the microscale thermophoresis experiments in Monolith NT.115 (NanoTemper) with the setting of 40% MST power and 40% excitation power at 25 °C ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
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bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration of EMC2•OG-488 was fixed at 50 nM and a dilution series of EMC8 or EMC9 ranging from 4 nM to 150 µM was used in the hydrophilic treated capillaries (NanoTemper Technologies). A Monolith NT.115 instrument (NanoTemper Technologies ...
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bioRxiv - Microbiology 2024Quote: ... using the Tycho NT.6 (NanoTemper). DSF experiments were set up to a final volume of 10 µl of buffer C containing the EccC5DUF construct at 2 µM in the absence or in the presence of ADP-AlF3 at 4 mM ...
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bioRxiv - Biochemistry 2020Quote: ... using a Tycho NT.6 instrument (Nanotemper), which allowed for determination of NT-mini’s unfolding temperatures in the different assayed conditions ...
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bioRxiv - Biochemistry 2023Quote: We used the Tycho NT.6 (NanoTemper Technologies) for label-free thermal shift analysis ...
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bioRxiv - Biochemistry 2021Quote: ... and RBD-71 measured using the Tycho NT.6 (NanoTemper).
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bioRxiv - Biochemistry 2024Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnA1 was analyzed using Tycho NT.6 (NanoTemper, Munich, Germany) by applying a standard capillary (10 µl ...
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bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
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bioRxiv - Biophysics 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15 mg ml−1 ...
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bioRxiv - Biochemistry 2022Quote: ... Data were acquired with a Tycho NT.6 equipment (NanoTemper Technologies). The emission fluorescence intensity was recorded at 330 nm and 35 ºC ...
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bioRxiv - Immunology 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15LmgLml−1 ...
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bioRxiv - Biochemistry 2024Quote: The thermal stability was performed using Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and kifunensine-treated SIDT1ECD were diluted to a final concentration of 0.25 mg/mL at various pH (pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... We used Tycho analysis (Tycho NT.6, NanoTemper Technologies GmbH, Munich, Germany) to follow the interaction between VcDnaB or EcDnaB and VcDciA ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Biochemistry 2023Quote: The thermal stability analysis was conducted using the Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and SIDT2ECD were diluted to a final concentration of 0.25 mg/mL at different pH values (pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were loaded into capillary tubes for Tycho NT.6 instrument (NanoTemper). Tryptophan fluorescence at emission wavelengths of 350 nm and 330 nm were measured as the sample temperature was ramped from 35 °C to 95 °C according to the preprogrammed instrument protocol ...
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bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
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bioRxiv - Biophysics 2023Quote: ... and experiments were carried out using the NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany). The temperature gradient was set to an increase of 30 °C/min from 35° C to 95° C ...
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bioRxiv - Microbiology 2019Quote: Thermal unfolding experiments of AtaC were performed with a Tycho NT.6 instrument (NanoTemper Technologies). The samples were heated in a glass capillary at a rate of 30 K/min and the internal fluorescence at 330 nm and 350 nm was recorded ...
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bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 µL reactions were incubated at room temperature for 5 min and 5 µL of the mixture was loaded into NT.115 capillaries (NanoTemper). MST was performed in Monolith NT.115 (NanoTemper ...
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bioRxiv - Cancer Biology 2023Quote: Label-free thermal shift assay experiments were performed with a Tycho NT.6 instrument (NanoTemper Technologies) based on a reported protocol 76 ...
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bioRxiv - Molecular Biology 2021Quote: Thermal stability was evaluated by nanoDSF using the Tycho NT.6 system (NanoTemper Technologies GmbH, München, Germany) 68 ...
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bioRxiv - Biophysics 2020Quote: Samples thermostability was measured by nano differential scanning fluorimetry using a Tycho NT.6 instrument (NanoTemper Technologies) that measures the intrinsic fluorescence from tryptophan and tyrosine residues detected at emission wavelengths of 350 and 330 nm ...
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bioRxiv - Plant Biology 2021Quote: ... 0.5 mM NaCl and run in duplicates in Tycho NT.6 capillaries (NanoTemper Technologies, Cat# TY-C001). Different protein solutions were prepared to reach a final CaCl2 concentrations of 0.01 mM ...
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bioRxiv - Biochemistry 2019Quote: The thermal stability of wild-type and variant RmtC proteins was assessed using a Tycho NT.6 instrument (NanoTemper) to ensure protein quality between different preparations of proteins and before/ after freezing ...
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bioRxiv - Biochemistry 2022Quote: ... tuberculosis DnaE1 was determined in the absence and presence of the ligand 6-thioinosine using a Tycho instrument (Nanotemper). 8 μM of M ...
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bioRxiv - Microbiology 2021Quote: The thermal stability of wild-type HigBHigA and HigB(L5ext)HigA were assessed using a Tycho NT.6 instrument (NanoTemper). Protein was heated at 0.1°C steps over a temperature range of 35°C to 95°C ...