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Citations for NanoTemper :
1 - 50 of 57 citations for 6 Fluoro 4 hydroxy 3 nitroquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
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bioRxiv - Biochemistry 2021Quote: Fluorescence labeling of the target protein (MDH) was performed by Red N-hydroxy succinimide (NHS) 2nd generation (NanoTemper Technologies GmbH, München, Germany) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... 6 (NanoTemper Technologies). Assays were done at 5.8 μM protein in 0.2 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Spike variants were diluted (0.15 mg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 6 (NanoTemper Technologies). Pre-dialyzed protein and mutant samples were diluted (∼0.5 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 (NanoTemper Technologies). Spike ectodomains were diluted to approximately 0.15LJmg/mL ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... 6 (NanoTemper Technologies, Germany). The measurements were performed at 0.1 mg/ml protein concentration in 15 mM Tris-SO4 pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... using the Tycho NT.6 (NanoTemper). DSF experiments were set up to a final volume of 10 µl of buffer C containing the EccC5DUF construct at 2 µM in the absence or in the presence of ADP-AlF3 at 4 mM ...
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bioRxiv - Biochemistry 2020Quote: ... using a Tycho NT.6 instrument (Nanotemper), which allowed for determination of NT-mini’s unfolding temperatures in the different assayed conditions ...
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bioRxiv - Biochemistry 2023Quote: We used the Tycho NT.6 (NanoTemper Technologies) for label-free thermal shift analysis ...
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bioRxiv - Immunology 2024Quote: ... Affinity Analysis 3 Software (Nanotemper) using a Kd fit model and constraining the target concentration.
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bioRxiv - Biochemistry 2021Quote: ... and RBD-71 measured using the Tycho NT.6 (NanoTemper).
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bioRxiv - Biochemistry 2024Quote: DSF assays were performed using Tycho NT.6 (NanoTemper Technologies). RBDs were diluted to approximatively 1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnA1 was analyzed using Tycho NT.6 (NanoTemper, Munich, Germany) by applying a standard capillary (10 µl ...
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bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
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bioRxiv - Biophysics 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15 mg ml−1 ...
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bioRxiv - Biochemistry 2022Quote: ... Data were acquired with a Tycho NT.6 equipment (NanoTemper Technologies). The emission fluorescence intensity was recorded at 330 nm and 35 ºC ...
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bioRxiv - Immunology 2023Quote: Thermal shift assay was performed using Tycho NT.6 (NanoTemper Technologies). Envelope ectodomain SOSIPs were diluted (0.15LmgLml−1 ...
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bioRxiv - Biochemistry 2024Quote: The thermal stability was performed using Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and kifunensine-treated SIDT1ECD were diluted to a final concentration of 0.25 mg/mL at various pH (pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
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bioRxiv - Molecular Biology 2022Quote: ... We used Tycho analysis (Tycho NT.6, NanoTemper Technologies GmbH, Munich, Germany) to follow the interaction between VcDnaB or EcDnaB and VcDciA ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Biochemistry 2023Quote: The thermal stability analysis was conducted using the Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and SIDT2ECD were diluted to a final concentration of 0.25 mg/mL at different pH values (pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were loaded into capillary tubes for Tycho NT.6 instrument (NanoTemper). Tryptophan fluorescence at emission wavelengths of 350 nm and 330 nm were measured as the sample temperature was ramped from 35 °C to 95 °C according to the preprogrammed instrument protocol ...
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bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
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bioRxiv - Biophysics 2023Quote: ... and experiments were carried out using the NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany). The temperature gradient was set to an increase of 30 °C/min from 35° C to 95° C ...
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bioRxiv - Microbiology 2019Quote: Thermal unfolding experiments of AtaC were performed with a Tycho NT.6 instrument (NanoTemper Technologies). The samples were heated in a glass capillary at a rate of 30 K/min and the internal fluorescence at 330 nm and 350 nm was recorded ...
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bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
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bioRxiv - Cancer Biology 2023Quote: Label-free thermal shift assay experiments were performed with a Tycho NT.6 instrument (NanoTemper Technologies) based on a reported protocol 76 ...
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bioRxiv - Molecular Biology 2021Quote: Thermal stability was evaluated by nanoDSF using the Tycho NT.6 system (NanoTemper Technologies GmbH, München, Germany) 68 ...
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bioRxiv - Biophysics 2020Quote: Samples thermostability was measured by nano differential scanning fluorimetry using a Tycho NT.6 instrument (NanoTemper Technologies) that measures the intrinsic fluorescence from tryptophan and tyrosine residues detected at emission wavelengths of 350 and 330 nm ...
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bioRxiv - Plant Biology 2021Quote: ... 0.5 mM NaCl and run in duplicates in Tycho NT.6 capillaries (NanoTemper Technologies, Cat# TY-C001). Different protein solutions were prepared to reach a final CaCl2 concentrations of 0.01 mM ...
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bioRxiv - Biochemistry 2019Quote: The thermal stability of wild-type and variant RmtC proteins was assessed using a Tycho NT.6 instrument (NanoTemper) to ensure protein quality between different preparations of proteins and before/ after freezing ...
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bioRxiv - Biochemistry 2022Quote: ... tuberculosis DnaE1 was determined in the absence and presence of the ligand 6-thioinosine using a Tycho instrument (Nanotemper). 8 μM of M ...
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bioRxiv - Microbiology 2021Quote: The thermal stability of wild-type HigBHigA and HigB(L5ext)HigA were assessed using a Tycho NT.6 instrument (NanoTemper). Protein was heated at 0.1°C steps over a temperature range of 35°C to 95°C ...
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bioRxiv - Biochemistry 2020Quote: DSF assays were performed on LH3 wild-type and mutants using a Tycho NT.6 instrument (NanoTemper Technologies GmbH, Germany). LH3 samples at a concentration of 1 mg/mL in a buffer composed of 25 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... TmrAB was diluted to 8.5 μM in SEC buffer and prepared in duplicate for measurement on a Tycho NT.6 (NanoTemper Technologies). Data are reported as the ratio of fluorescence intensity at 350 nm and 330 nm normalized to the minimum and maximum values of the whole dataset.
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bioRxiv - Microbiology 2021Quote: ... Quality control during protein purification was carried out using reducing and non-reducing SDS-PAGE analysis and differential scanning fluorimetry (DSF) with a Tycho NT.6 instrument (Nanotemper). All samples were concentrated to 1 mg mL-1 ...
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bioRxiv - Biochemistry 2022Quote: ... NCS-1ΔH10/Ric-8A-P2 peptide and NCS-1ΔH10/Ric-8A-P3 peptide were performed using a Tycho NT.6 instrument (NanoTemper Technologies). Proteins at 10 μM in their corresponding final buffers (see above ...
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bioRxiv - Biochemistry 2023Quote: ... Thermal stability in the presence and absence of various potential ligands was measured using a Tycho NT.6 instrument (Nanotemper).
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and Spike RBD mixed with W25 (2 μM each) were prepared and loaded into Capillaries Tycho NT.6 (TY-C001, NanoTemper Technologies GmbH). The Tycho instrument applied a quick thermal ramp from 35°C to 95°C with a heating rate of 30°C/min and the unfolding of proteins were monitored through changes in the 350 nm / 330 nm fluorescence emission ratio ...
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bioRxiv - Molecular Biology 2020Quote: The binding of W25 and the structural integrity of Spike RBD was verified using a label-free thermal shift assay with Tycho NT.6 (Nanotemper Technologies GmbH) using intrinsic tryptophan and tyrosine fluorescence ...
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bioRxiv - Plant Biology 2022Quote: ... Thermal unfolding of G-actin (2 μM) and F-actin (2 μM) was performed with the Tycho NT.6 (Nanotemper, Munich, Germany) according to the manufacturer’s instructions in G-buffer (5 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The thermal stability of Conk-C3 derivatives was determined by differential scanning fluorimetry on a NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany) with a back-reflection aggregation detection at a range from 35 to 95°C and with a heating rate of 20°C min-1 ...
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bioRxiv - Biochemistry 2023Quote: ... were incubated with 0 mM to 50 mM GMP/GDP-mannose at room temperature for 15 minutes before measuring the Tm using a Tycho NT.6 instrument (NanoTemper Technologies, Germany). All experiments were done in two biological repeats and three technical repeats ...
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bioRxiv - Microbiology 2020Quote: ... Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies), running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then loaded into 3 standard capillaries for triplicate readings in a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, Germany). Experiments were run using 40% excitation power ...
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bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...