Labshake search
Citations for NanoTemper :
1 - 50 of 65 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... The 16 capillary tubes (NanoTemper Technologies) were inserted into each sample tube to enable sample entry into the capillary ...
-
bioRxiv - Biophysics 2020Quote: ... 1 mM CaCl2 and 0.05 % (v/v) Tween-20 using a Nanotemper Monolith NT.115 (Nanotemper). To measure the affinity of zGFRα1D1-3-zGDNFmat ...
-
bioRxiv - Cell Biology 2019Quote: ... Tubes were shifted to RT and mixtures were transferred to 16 standard capillaries (Nanotemper, Germany) according to the manufacturer’s manual ...
-
bioRxiv - Immunology 2024Quote: ... Affinity Analysis 3 Software (Nanotemper) using a Kd fit model and constraining the target concentration.
-
bioRxiv - Biochemistry 2023Quote: ... a 16-point affinity measurement was performed in duplicates using the Dianthus (NanoTemper Technologies GmbH, Germany) instrument ...
-
bioRxiv - Biophysics 2022Quote: ... The mixed RBD+ACE2 samples were separately loaded into 16 premium glass capillaries (NanoTemper Technologies, MO-K025). The 16 capillaries were then placed in the reaction chamber in the order from low to high concentration ...
-
bioRxiv - Molecular Biology 2023Quote: The DNA binding affinities of HBb to the 80 bp (9, 37) and 80 bp-GC50 oligonucleotides were measured using the Monolith NT.115 instrument (NanoTemper Technologies). MST data were analyzed using the associated MO.Control software.
-
bioRxiv - Biophysics 2021Quote: ... Measurements were carried out in MST-buffer (20 mM phosphate, 120 mM NaCl, at pH 7.4, in 0.005% Tween-20) using premium capillaries (Nanotemper). Proteins were labeled with RED-tris-NHS dye following supplier protocol ...
-
bioRxiv - Biophysics 2020Quote: Thermal unfolding of BG505-SOSIP was determined with a heating ramp of 1°C/min and 20% sensitivity setting using high-sensitivity capillaries (NanoTemper Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... The mixtures were incubated at room temperature for 10 min and transferred to 16 standard capillaries (Nanotemper, Germany). Data were acquired using medium MST power ...
-
bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
-
bioRxiv - Biochemistry 2022Quote: ... Then the mixture was incubated overnight at 16 °C followed by centrifugation at 15,000 g for 15 min before loaded into the Premium Capillaries (NanoTemper Technologies). The thermophoresis analysis ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 nM of the labeled ARC4 and 16 two-fold dilution series of SPIDER were loaded into sixteen premium capillaries (NanoTemper Technologies GmbH ...
-
bioRxiv - Biochemistry 2023Quote: ... Serial dilutions of TAM were made in 16 different capillaries containing 50 µM PhoR-GFP (such that the fluorescence in the NanoTemper™ Monolith is in the range of 1200-1400 U) ...
-
bioRxiv - Cell Biology 2021Quote: ... 10 μl of the protein samples at 10 μM were measured in triplicates with a temperature ramp set to 1 °C/min from 20 °C-80 °C using standard treated capillaries (NanoTemper Technologies (#PR-C002)) ...
-
bioRxiv - Plant Biology 2022Quote: The purified protein in PBS at a concentration of ∼16 µM was loaded into capillaries (nanoDSF grade standard, NanoTemper GmbH, Munich, Germany) and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.05% Tween-20 in premium coated capillaries (NanoTemper Technologies GmbH; MO-K025) using red LED source ...
-
bioRxiv - Microbiology 2021Quote: The experiments were performed at 20°C on a Monolith NT.115 (Nanotemper) with Premium Coated (Nanotemper ...
-
bioRxiv - Molecular Biology 2020Quote: ... supplemented with Neu5Ac (20 mM) Data analysis was performed using PR.ThermControl software (NanoTemper Technologies) where an apparent melting point (Tm ...
-
bioRxiv - Microbiology 2021Quote: ... MST/TRIC measurements were performed at 20°C using a Monolith NT.115 instrument (NanoTemper) at 25% LED power and 20% MST laser power ...
-
bioRxiv - Biochemistry 2022Quote: ... thermal melting was performed from 20 to 90°C using a PrometheusNT.48 DSF (Nanotemper) using high sensitivity capillaries ...
-
bioRxiv - Immunology 2024Quote: ... Each 20 μL sample was split between two Prometheus Grade Standard Capillaries (Nanotemper, Munich, Germany) and placed in the capillary tray of a Prometheus Panta (Nanotemper ...
-
bioRxiv - Microbiology 2023Quote: ... Light scattering measurements were performed at 20 °C using Prometheus Panta (NanoTemper Technologies GmbH, Munich, Germany).
-
bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20% LED power in standard capillaries at 25°C on a Monolith NT.115 device (NanoTemper Technologies). The data was analyzed using the MO.affinity analysis software (V2.3 ...
-
bioRxiv - Microbiology 2020Quote: ... Microscale thermophoresis was completed in 3 independent repeat experiments on a Monolith NT.115 instrument (NanoTemper Technologies), running MO.Control software with settings of 60-80% excitation power and 40% MST power at room temperature.
-
bioRxiv - Biochemistry 2022Quote: ... All reactions were incubated for 20 minutes at 25 ºC before loading into premium glass capillaries (NanoTemper Technologies). Microscale thermophoresis (MST ...
-
bioRxiv - Bioengineering 2023Quote: ... Then 20 μL sample was loaded into standard treated capillaries for measurements on a Monolith NT Automated (Nanotemper).
-
bioRxiv - Molecular Biology 2024Quote: ... and the buffers were replaced by PBS buffer with 0.005% Tween-20 (PBST) using column A (NanoTemper Technologies). Next ...
-
bioRxiv - Biochemistry 2024Quote: ... and the thermal stability was monitored from 20°C to 90°C at a heating rate of 1.5°C/min using the PR.ThermControl software (NanoTemper).
-
bioRxiv - Microbiology 2022Quote: ... to 0.1 µM purified ClpC1NTD-FITC was assayed in CPD supplemented with 0.05% Tween 20 and 8 mg/ml BSA by microscale thermophoresis using a Monolith NT.115 (NanoTemper) (Jerabek-Willemsen et al. ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Experiments were run at 20% excitation and high MST power at 23.0°C on a Monolith NT.115Pico (NanoTemper). Each experimental compound was run in triplicate.
-
bioRxiv - Biochemistry 2021Quote: ... The final reaction included 20 uM RED-NHS labelled untagged Spp1 (labelling was performed as in manufacturer’s protocol-Nanotemper) and titration series of MBP-Mer2 constructs (concentrations calculated based on oligomerization stage of Mer2) ...
-
bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
-
bioRxiv - Microbiology 2019Quote: ... CqsR-LBD was diluted to 20 μM and labeled using the Monolith NT Protein Labeling Ki RED-NHS (NanoTemper Technologies). Different concentrations of ethanolamine were incubated with 20 nM working stock solutions of labeled protein in the dark for 30 min at 4° C ...
-
bioRxiv - Biophysics 2023Quote: ... 0.2 mM ATP) with 0.05 % Tween-20 on a Monolith NT.115 Microscale Thermophoresis (MST) device using standard treated capillaries (NanoTemper Technologies). Actin (final labelled-actin concentrations adjusted to 60 nM ...
-
bioRxiv - Biochemistry 2019Quote: ... was monitored during unfolding over a linear temperature gradient (35-95 °C) over 3 minutes on a Tycho NT.6 instrument (NanoTemper). The temperature at which a transition occurs in a plot of the first derivative of the fluorescence ratio (350/330 nm) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then loaded into 3 standard capillaries for triplicate readings in a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, Germany). Experiments were run using 40% excitation power ...
-
bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
-
bioRxiv - Biophysics 2022Quote: ... HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml) were collected in glass capillaries (Standard Capillaries Prometheus NT.48, NanoTemper), capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
-
bioRxiv - Microbiology 2019Quote: ... Measurements were performed in standard capillaries with a Monolith NT.115 Pico system at 20% excitation power and 40% MST power (NanoTemper Technologies).
-
bioRxiv - Biochemistry 2021Quote: ... Measurements were conducted at 40% MST power and light-emitting diode (LED)/excitation power setting 20% in Premium Coated capillaries on the Monolith NT.115 at 25°C (Nanotemper Technologies). Each experiment was performed in at least three replicates ...
-
bioRxiv - Biochemistry 2021Quote: ... The mixtures were loaded into capillaries and measured at ambient temperature by using 20% LED and medium MST power in a Monolith NT.115 system (NanoTemper Technologies). The data were analyzed using NanoTemper analysis software (v.1.2.101).
-
bioRxiv - Molecular Biology 2023Quote: ... All mixtures were prepared in 0.05% Tween 20 in PBS and the measurement was carried out in Monolith NT™ Standard capillaries (NanoTemper Technologies). Excitation power and MST power of Microscale Thermophoresis instrument (Monolith NT.115 ...
-
bioRxiv - Biochemistry 2022Quote: ... 13000 rpm), filled into Prometheus high-sensitivity capillaries (10 μL each, n=3) and transferred to a Prometheus Panta instrument (NanoTemper Technologies). DLS analysis of BbHtrA S/A rh and PDI were determined using Prometheus Panta’s size analysis function.
-
bioRxiv - Microbiology 2022Quote: ... The labeled protein was 1:1 incubated with a series dilution of ABT199 (4 uM to 0.122 nM) to perform the microscale thermophoresis experiments in Monolith NT.115 (NanoTemper) with the setting of 40% MST power and 40% excitation power at 25 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... for 30 min and centrifuged at 14,000 rpm for 10 min at 4°C before loading into Monolith NT.115 premium capillaries (NanoTemper). Samples were loaded into the Monolith NT.115 device (NanoTemper ...
-
bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
-
bioRxiv - Biochemistry 2020Quote: ... The concentration of EMC2•OG-488 was fixed at 50 nM and a dilution series of EMC8 or EMC9 ranging from 4 nM to 150 µM was used in the hydrophilic treated capillaries (NanoTemper Technologies). A Monolith NT.115 instrument (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 20k ×g at 4 °C for 5 min prior to loading into standard-coated capillary tubes (NanoTemper, GmbH, Munich, Germany). Microscale thermophoresis data were collected on a Monolith NT.115 (NanoTemper ...