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Citations for NanoTemper :
151 - 183 of 183 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... ColG variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper Technologies, Germany). After labeling ...
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bioRxiv - Cancer Biology 2024Quote: ... 90 µl of 200 nM protein mixed 1:1 with 100 nM RED-tris-NTA 2nd generation dye (MO-L018, Nanotemper Technologies) and incubated at room temperature for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Purified TRIM21 PRYSPRY domain was labeled using Protein Labeling Kit RED-NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH, Munich, Germany). Labeled protein was diluted to 50 nM in PBST (137 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Thermal unfolding was followed by differential scanning fluorimetry (DSF) by measuring intrinsic protein fluorescence using a Prometheus NT.48 (NanoTemper Technologies). Aliquots (10 μl ...
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bioRxiv - Biochemistry 2024Quote: The binding affinity of each recombinant TrK13 protein for heme was evaluated by MST analysis using the Monolith NT.115 instrument (NanoTemper Technologies). The recombinant TrK13 proteins (50 nM ...
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bioRxiv - Plant Biology 2024Quote: ... Purified insect cell-expressed RACK1A was fluorescently labeled with Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies GmbH MO-L011) via amine conjugation ...
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bioRxiv - Biophysics 2023Quote: ... and the intrinsic protein fluorescence was recorded at 330 and 350 nm using the Prometheus NT.Plex nanoDSF device (NanoTemper, Munich, Germany).
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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bioRxiv - Microbiology 2022Quote: ... were labeled with the Monolith Protein Labeling Kit RED-NHS 2nd generation according to the recommendations of the manufacturer (NanoTemper Technologies). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The labeled aptamer/protein mixture is incubated for 30 min at room temperature before loading them in Monolith NT.115 Capillary (NanoTemper, Germany) and determining binding affinity using Monolith machine.
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bioRxiv - Microbiology 2022Quote: Purified WT or mutant NSP12 recombinant proteins were labeled with Cytidine-5 dye using the Monolith His-Tag labeling kit (NanoTemper Technologies), and then labeled NSP12 proteins were diluted to 700 nM in PBST buffer ...
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bioRxiv - Biochemistry 2023Quote: ... His LRRK2 Armadillo (1–552) K439E and E240R were labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (NanoTemper Technologies). For all experiments ...
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bioRxiv - Biochemistry 2023Quote: The thermal stability of wt-JDBD and mutant proteins was measured in the Prometheus NT.48 nanoDSF (nano differential scanning fluorescence) instrument (NanoTemper Technologies). For each protein ...
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bioRxiv - Biophysics 2023Quote: The recombinant RAD52 FL protein was labeled with the Monolith labeling kit RED-NHS (ammine dye NT-647-NHS) according to manufacturer indications (NanoTemper Technologies). Before MST experiments ...
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bioRxiv - Molecular Biology 2023Quote: The melting temperature Tm of a TadA protein was determined using nano differential scanning fluorimetry (nanoDSF,Nanotemper Prometheus NT.48 system) [40] ...
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bioRxiv - Molecular Biology 2024Quote: The protein thermal stability studies for all IFIT samples and complexes were performed using Prometheus NT.48 nanoDSF device (NanoTemper Technologies) with simultaneous Dynamic light scattering (DLS ...
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bioRxiv - Biochemistry 2020Quote: 50 µg synthesized sP26 (Davids Biotechnologie, Regensburg, Germany) was fluorescently labeled with the Monolith NT Protein Labeling Kit RED (NanoTemper, Munich, Germany) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Fluorescence labeling of the target protein (MDH) was performed by Red N-hydroxy succinimide (NHS) 2nd generation (NanoTemper Technologies GmbH, München, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: The thermal unfolding experiments of the IgLC samples as a function of protein and denaturant concentration were performed with a Prometheus Panta instrument (Nanotemper, Munich, Germany). This is a microcapillary-based (10 μl sample per capillary ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µM His6-SUMO-AGO1-NcGN was orthogonally labeled on Cystein-192 using the Monolith NT™ Protein Labeling Kit Red – Maleimide (NanoTemper Technologies). The labeling was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Each protein dilution was mixed with one volume of labeled DNA and filled into Monolith NT standard treated capillaries (NanoTemper Technologies GmbH). Thermophoresis was measured using a Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Cell Biology 2024Quote: MST measurements were carried out using premium-coated capillaries to reduce non-specific interaction of the proteins with the glass surface on a Monolith NT.115 red-blue (Nanotemper, Munich, Germany), as described previously (Zuidema et al. ...
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bioRxiv - Plant Biology 2022Quote: The purified protein in PBS at a concentration of ∼16 µM was loaded into capillaries (nanoDSF grade standard, NanoTemper GmbH, Munich, Germany) and a melting curve was determined by using the nanoDSF Prometheus NT.48 instrument (NanoTemper GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture of 16 serial diluents of the ligand and labelled protein was filled into the capillary tubes (Nanotemper, MO-K022) and loaded in the Monolith NT.115 instrument accordingly ...
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bioRxiv - Biophysics 2024Quote: ... (H2aG129C-H2b) dimer and (H3-H4)2 tetramer were labeled using cysteine reactive Monolith NT™ Protein Labeling Kit RED-MALEIMIDE (NanoTemper Technologies) for interaction studies of various αSyn constructs ...
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bioRxiv - Biophysics 2020Quote: ... PD-1 and its mutant were fluorescently labelled according the standard labeling protocol of the NanoTemper Protein Labeling Kit RED-NHS (L001-NanoTemper Technologies, Munich, Germany). In MST experiments ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant human FTO protein was labeled with His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper Technologies GmbH; MO-L008). The labelled FTO protein (target ...
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bioRxiv - Biochemistry 2023Quote: The BD5.1 protein was labelled with the Monolith His-Tag Labeling Kit (RED-tris-NTA 2nd Generation kit, NanoTemper Technologies, Catalog# MO-L018) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Recombinant METTL16 MTD (residues 1-291) protein was labelled via His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH, Germany). The labelled protein was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Biophysics 2024Quote: The (I91)2-ClfAN2N3-(I91)2 polyprotein was fluorescently labeled by utilizing the commercial protein Labeling Kit RED-NHS 2nd Generation (Nanotemper Technologies GmbH, Munich, Germany). The dye carries a reactive NHS-ester group that reacts with primary amines in the protein to form a covalent bond ...
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bioRxiv - Plant Biology 2022Quote: ... 50 nM of protein sample in PBS together with indicated concentrations of nucleotides were loaded into standard Monolith NT.115 capillaries (NanoTemper Technologies GmbH, Munich, Germany). MST measurement was performed using the Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... histidine tagged HMGB1 was labeled by the his-tag specific NT-647 dye (Monolith NTTM Protein Labelling Kit RED-NHS, NanoTemper® Technologies GmbH, Mu□nchen, Germany), for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: Fluorophore-labeled protein was incubated with various ligands at room temperature for 15 minutes before measuring on Monolith NT.115 (NanoTemper, 80% LED power, 40% MST power). For competition assays ...