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Citations for NanoTemper :
101 - 150 of 219 citations for Recombinant Human ERBB2 protein Fc tagged R PE labeled since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
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bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
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bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
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bioRxiv - Biochemistry 2024Quote: ... fluorescein-labeled P2X3 was prepared in buffer D according to the manufacturer’s instructions (Red Tris 2nd generation dye, NanoTemper). A 2-fold dilution series of camlipixant was then prepared in buffer D ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
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bioRxiv - Cancer Biology 2021Quote: Binding of purified recombinant KIX with fluorescein isothiocyanate (FITC)-conjugated peptides was measured by microscale thermophoresis using the Monolith NT.115 (NanoTemper Technologies). Assays were conducted in 50 mM sodium phosphate ...
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bioRxiv - Immunology 2024Quote: The binding interaction between BTN2A2-His and CD45RO-Fc was measured by Microscale Thermophoresis (MST) using the Monolith NT.115 instrument (Nanotemper Technologies, München, Germany) as described (Wienken et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Control experiments included measurements of the binding of the RED-tris-NTA dye to His-DJ-1 and of a dye-labeled AptamerCy5 to AMP using a manufacturer kit (Monolith NT.115 Control Kit RED, NanoTemper).
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bioRxiv - Cell Biology 2023Quote: ... 10 nM of the labeled ARC4 and 16 two-fold dilution series of SPIDER were loaded into sixteen premium capillaries (NanoTemper Technologies GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... AcrVIB1-His was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (Nanotemper, MO-L018) according to the manufacturer‘s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The TACC3 domain was fluorescently labeled via NHS coupling using the standard NHS Red labeling kit following the manufacturers protocol (Nanotemper, Germany). MST experiments were recorded on a Nanotemper Monolith instrument using a concentration of the fluorescently labeled TACC domain of 600 nM while the concentration of the H5 peptide was varied in the range of 0–400 µM ...
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bioRxiv - Biophysics 2024Quote: ... 200 nM of the protein was labeled with 100 nM NT-647 dye of Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, Germany) and incubated at room temperature for 30 minutes followed by centrifugation at 15000Xg for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018, NanoTemper Technologies, GmbH, Munich, Germany) per the manufacturer’s instructions with slight modifications (43) ...
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bioRxiv - Microbiology 2024Quote: ... alleles of phage proteins containing C-terminal 6×His tags were labeled using a Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (Nanotemper: Cat# MO-L018) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
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bioRxiv - Immunology 2022Quote: Protein-protein interactions were analyzed via microscale thermophoresis on a Monolith NT.115 instrument equipped with green/red filters (NanoTemper Technologies, Munich, Germany). Measurements were performed at 25°C at both 40% and 80% MST power ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: We measured the binding affinity of MTBDs (fluorescently labeled target) to microtubules (unlabeled ligand) using a Monolith NT.115 MST system (NanoTemper Technologies, GmbH, Munich, Germany). We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018 ...
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bioRxiv - Neuroscience 2023Quote: ... into 50 nM of fluorescently labeled Munc13-1 constructs in a premium-coated glass capillary using a Monolith NT.115 (Nanotemper Technologies, San Francisco, CA).
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bioRxiv - Biochemistry 2020Quote: ... Thermally-driven protein unfolding was monitored in Tycho quartz capillaries (Nanotemper) using a Tycho NT.6 instrument (Nanotemper) ...
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bioRxiv - Microbiology 2022Quote: ... Protein quality was verified using a Tycho NT.6 system (NanoTemper) and ACE2 activity assay kit (SensoLyte ...
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bioRxiv - Biophysics 2024Quote: ... Protein Labeling Kit RED-NHS 2nd Generation was purchased from NanoTemper Technologies (München ...
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bioRxiv - Biophysics 2024Quote: ... CRBN protein was labelled with RED-Maleimide 2nd Generation dye (NanoTemper) optimized for degree-of-labeling of 1:1 molar ratio calculated by nanodrop ...
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bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
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bioRxiv - Immunology 2022Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95 °C at a heating rate of 1 °C per min ...
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bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
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bioRxiv - Biophysics 2021Quote: ... or Monolith NT™ (Protein Labeling Kit Blue-NHS, NanoTemper GmbH, Germany). The labeling was achieved by incubating 115 μM of protein and 350 μM of dye (465 μl reaction volume ...
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... protein quality was assessed using a Tycho NT.6 (NanoTemper, Munich, Germany). Labeled protein was dissolved in MST buffer (50mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2023Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1°C/min ...
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bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Monolith protein labeling kit RED-NHS 2nd generation was purchased from NanoTemper Technologies (Munich ...
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bioRxiv - Immunology 2024Quote: Protein thermostability was evaluated with a Prometheus NT.48 instrument (NanoTemper Technologies). Proteins at a concentration of 1 mg/mL were loaded to the grade capillaries and the intrinsic fluorescence signal was measured while temperature was increased by 1 °C/min ...
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bioRxiv - Biochemistry 2024Quote: ... Protein unfolding was monitored using a Prometheus NT.48 instrument (NanoTemper Technologies) by measuring absorbance at 330 and 350 nm (with 20% excitation) ...
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bioRxiv - Biochemistry 2022Quote: Protein thermal stability was measured using nanoDSF on a Prometheus panta instrument (NanoTemper) (39) ...
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bioRxiv - Biochemistry 2020Quote: ... 10μL of the diluted protein was loaded into NanoDSF Grade Standard Capillaries (NanoTemper) and melting temperatures were determined using a Prometheus NT4.8 NanoDSF instrument according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM NaCl using the Monolith NT Protein Labelling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: We determined protein melting temperatures using the Prometheus NT.48 instrument (NanoTemper Technologies). Purified FabI was diluted to a concentration of 25 μM in 50 nM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein series was loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and thermophoresis was measured using MO.Control v1.6.1 software ...
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bioRxiv - Cell Biology 2023Quote: IRE1αLD was labelled using the Monolith NT protein labelling Kit Red-Maleimide (NanoTemper). The concentration of the labelled IRE1αLD was kept constant at 10 nM ...
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bioRxiv - Biochemistry 2023Quote: Protein Labeling Kit RED-maleimide 2nd Generation (cat# MO-L014; NanoTemper Technologies GmbH) was used for covalent labeling of LC3A cystine residues ...
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bioRxiv - Immunology 2024Quote: Protein thermal-melting profiles were obtained using a Prometheus NT.48 Instrument (NanoTemper). Proteins were diluted in PBS to a concentration of 0.1 mg/mL and loaded into glass capillaries (NanoTemper) ...
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bioRxiv - Microbiology 2024Quote: The protein thermal stability measurements were performed using Tycho NT.6 (NanoTemper Technologies). The purified protein samples were diluted in the test condition buffers to a range of concentrations from 1 to 2 mg mL-1 ...
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bioRxiv - Biophysics 2022Quote: Recombinantly purified protein constructs were labelled with RED-tris NTA dye (NanoTemper Technologies GmbH) in PBS to a concentration of 100 nM ...
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bioRxiv - Pathology 2021Quote: ... Protein quality was determined using a Tycho NT.6 (Figures S4 & S5) (NanoTemper Technologies). Human hnRNPC was purchased from Abnova (catalog# H00003183P01S.)
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bioRxiv - Molecular Biology 2022Quote: SAE1/SAE2 was labelled using Protein Labelling Kit RED-NHS 2nd Generation (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer ...
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bioRxiv - Biophysics 2022Quote: JIP3-Nter fragments were labelled using the Protein Labelling Kit Blue-NHS (NanoTemper Technologies). The labelling reaction was performed according to the manufacturer’s instructions in the supplied labelling buffer applying a concentration of 20 μM protein (molar dye:protein ratio ≈ 3:1 ...
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bioRxiv - Biophysics 2024Quote: ... The melting temperature for characterization of protein thermal stability were done using NanoDSF (NanoTemper) with protein concentration at 0.2mg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... mixed 1:1 with the labelled protein and loaded into standard capillaries (NanoTemper Technologies). MST measurements were recorded using 20 % LED power and 20 to 80 % MST power and analysed using MO ...
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bioRxiv - Biochemistry 2022Quote: ... Protein folding and quality control was accomplished using nDSF on a Tycho NT.6 (NanoTemper) which monitors protein thermal unfolding using intrinsic fluorescence at 330 and 350 nm ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monolith Protein Labeling Kit RED-NHS 2nd Generation kit (NanoTemper MO- L011) to label ∼1 lysine per Fcy1 monomer with a fluorophore following the manufacturer’s instruction ...