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Citations for NanoTemper :
101 - 150 of 184 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (Nanotemper Technologies). For all experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by labelling with 30uM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at RT for 30 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein binding was measured by the microscale thermophoresis technique on a Monolith NT.115 instrument (Nanotemper). Protein (10 nM ...
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bioRxiv - Cell Biology 2021Quote: ... The RNA-protein mixtures were analyzed in premium capillaries using the Monolith NT.115T MST (NanoTemper Technologies). Measurements were carried out at 25 °C ...
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bioRxiv - Biochemistry 2022Quote: ... K17A and K18A were labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (Nanotemper Technologies). For all experiments ...
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bioRxiv - Biochemistry 2022Quote: NanoDSF and light scattering of purified proteins were measured with a Prometheus NT.48 (Nanotemper, Munich, Germany), equipped with back-reflection optics or with a Prometheus Panta (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... Then the proteins were loaded onto Prometheus NT.48 nanoDSF Grade Standard Capillaries (PR-C002, NanoTemper Technologies), the fluorescence signal was recorded under a temperature gradient ranging from 25 to 90°C (incremental steps of 0.5°C min-1) ...
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bioRxiv - Biochemistry 2023Quote: Protein thermal stability was determined by differential scanning fluorimetry (nanoDSF) on Prometheus NT.48 instrument (Nanotemper Technologies) in nanoDSF grade standard capillaries filled with the samples ...
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bioRxiv - Cell Biology 2023Quote: ... NRP-1 b1b2 domains were labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies, Munich, Germany). The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The Csy complexes were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein and compound mixtures were loaded into standard treated capillaries (MO-K022, NanoTemper Technologies GmbH, Munich, Germany). Measurements were performed on a Monolith NT.115 with blue/red filters (NanoTemper Technologies GmbH ...
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bioRxiv - Biochemistry 2024Quote: Thermal stability of proteins was analyzed using nanoscale differential scanning fluorimetry with a Prometheus NT.48 (NanoTemper) device ...
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Undruggable oncoproteins cMyc and NMyc bind to mediator of transcription with superior high affinitybioRxiv - Biochemistry 2024Quote: ... The quality of the protein was determined using Differential Scanning Fluorimetry (Prometheus NT.48, NanoTemper Technologies GmbH). The uniform labelling of KIX domain was confirmed by MALDI-TOF MS.
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bioRxiv - Biochemistry 2021Quote: Protein thermal stability was measured in a label-free fluorescence assay using a Prometheus NT.48 (NanoTemper, Germany). Briefly ...
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bioRxiv - Biochemistry 2020Quote: His6-GlnK1 was purified and labeled using Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) (DOL ...
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bioRxiv - Microbiology 2021Quote: ... TgPRP4K protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies). The labeled TgPRP4K protein was adjusted to 100 nM with a buffer containing 30 mM Hepes (PH 7.5) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein (200 nM) was fluorescently labeled using a RED-tris-NTA 2nd generation dye (NanoTemper Technologies GmbH) and a 1:1 dye/protein molar ratio in the reaction buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... between protein:compound sample and a protein only sample at 1.5-2-5 sec is calculated and plotted through MO.Affinity analysis v2.3 (NanoTemper Technologies) and GraphPad Prism 8.0.0 (GraphPad Software ...
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bioRxiv - Biochemistry 2022Quote: ... Labeling of RIP2K1-300 was performed with Monolith Protein labeling kit RED-NHS 2nd generation (MO-L011, Nanotemper) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... followed by labeling with 30 μM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at Room Temperature (RT ...
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bioRxiv - Immunology 2022Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Immunology 2023Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... followed by labeling with cysteine-reactive dye (30 mM; Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper) and incubated for 30 min at room temperature (RT ...
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bioRxiv - Biochemistry 2024Quote: Wt and P323L variants of nsp12 were labeled with NHS-fluorescent label utilizing the Protein labelling kit (Nanotemper). Labeled proteins were then diluted to either 105nM for protein-protein interactions or 52.5 nM for complex formation and utilized as a target molecule ...
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bioRxiv - Cell Biology 2024Quote: Purified protein was labeled using the His-Tag labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, MO-L018) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... The onset of scattering and melting temperatures based on the change of intrinsic protein fluorescence (ratio 350 nm/330 nm) were calculated in PR.ThermControl software (NanoTemper).
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bioRxiv - Biochemistry 2019Quote: The thermal stability of wild-type and variant RmtC proteins was assessed using a Tycho NT.6 instrument (NanoTemper) to ensure protein quality between different preparations of proteins and before/ after freezing ...
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bioRxiv - Biochemistry 2020Quote: Purified His6-GlnA1 was fluorescently labeled using the Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) with buffer B (50 mM HEPES ...
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bioRxiv - Cancer Biology 2019Quote: ... was labeled for microscale thermophoresis (MST) using the Monolith NT Protein RED-NHS Labeling Kit (NanoTemper Technologies, München, Germany) according to the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... MBP-PDF2(START)L467P were labeled in 50 mM sodium phosphate buffer (pH 7.4) supplemented with 500 mM NaCl using Monolith Protein Labeling kit RED-MALEIMIDE (NanoTemper) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... W25 was fluorescently labeled with the Monolith Protein Labeling Kit RED – NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH) following the protocol established in the manual ...
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bioRxiv - Molecular Biology 2022Quote: SiaQM in amphipol was labelled at a ratio of 0.8 dye / 1 protein following the protocol of the RED-NHS 2nd generation labelling kit (NanoTemper). Labelled SiaQM was held constant at 20 nM and SiaP was titrated at 16 concentrations starting at 2,900 μM and serially diluting 2-fold to 90 nM ...
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bioRxiv - Biophysics 2022Quote: 6.6 µM GyrA14 (in 1X PBS) was labeled using the RED-NHS Monolith™ Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: Protein sample preparation and measurements were performed according to the protocol from Monolith NT (NanoTemper Technologies GmbH, Munich, Germany), employing quality controls as outlined by Sedivy 202143 ...
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bioRxiv - Systems Biology 2023Quote: ... NSP10 protein was fluorescently labeled using N-Hydroxysuccinimide (NHS)-ester fluorophores according to the manufacturer’s instructions (Protein Labeling Kit RED-NHS 2nd Generation, Nanotemper). Labeling was performed in the NSP16 protein storage buffer (20 mM HEPES pH 7.5 and 0.3 M NaCl) ...
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bioRxiv - Microbiology 2021Quote: ... The labelling of NyxA was performed by covalent coupling of the lysines of the protein to a fluorophore using the RED-NHS (Nanotemper) Protein Labeling Kit ...
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bioRxiv - Biochemistry 2020Quote: ... This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001) following the manufacturer instruction (Nanotemper) and diluted in the purification binding buffer (see above ...
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bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: NanoDSF Systems were conducted to measure the thermostability and aggregation of purified protein using Prometheus NT.48 instrument (NanoTemper Technologies). A 10 μL aliquot of sample diluted to a concentration of 5 mg/mL were applied to quartz capillary cassette and placed into card slot ...
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bioRxiv - Microbiology 2019Quote: ... CqsR-LBD was diluted to 20 μM and labeled using the Monolith NT Protein Labeling Ki RED-NHS (NanoTemper Technologies). Different concentrations of ethanolamine were incubated with 20 nM working stock solutions of labeled protein in the dark for 30 min at 4° C ...
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bioRxiv - Biochemistry 2021Quote: C-terminally His6-tagged proteins were labeled using the Monolith His-tag labeling kit RED-tris-NTA (Nanotemper #MO-L008) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
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bioRxiv - Biochemistry 2022Quote: The amine residues of purified KsgA were fluorescently labeled with NHS red using the Protein Labeling Kit RED-NHS 2nd Generation (Cat # MO-L011 Nanotemper). The labeling reaction was performed according to the manufacturer’s protocol by mixing KsgA at a final concentration of 20 μM with a 3-fold molar excess of dye at room temperature for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The TPR-CHAT and its mutants were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies with an excitation wavelength of 280 nm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies (Munchen ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing varying concentrations of protein were loaded into glass capillaries and applied to the nanoDSF device Prometheus NT.48 (Nanotemper). The samples were heated from 20 to 90°C with a slope of 1.5°C/min and the unfolding of the proteins was observed by detecting shifts in the fluorescence at 330 and 350 nm ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...