Labshake search
Citations for NanoTemper :
101 - 150 of 183 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Purified protein was labeled using the His-Tag labeling Kit RED-tris-NTA 2nd Generation (Nanotemper, MO-L018) following the manufacturer’s directions ...
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bioRxiv - Biochemistry 2024Quote: ... followed by labeling with cysteine-reactive dye (30 mM; Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper) and incubated for 30 min at room temperature (RT ...
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bioRxiv - Immunology 2022Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Immunology 2023Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Immunology 2024Quote: ... Proteins were diluted in PBS to a concentration of 0.1 mg/mL and loaded into glass capillaries (NanoTemper). Samples were then heated at a rate of 1 °C per min in a gradient that ranged from 20 to 95 °C while intrinsic fluorescence was recorded (350 nm and 330 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... 12 serial dilutions of the ligand and labelled protein were loaded into the standard capillaries (Nanotemper, MO-K022) and analyzed using the Monolith NT.Automated instrument ...
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bioRxiv - Biophysics 2024Quote: ... Protein fluorescence at 350 nm (F350) and 330 nm (F330) was measured by a Prometheus nDSF (NanoTemper Technologies) device over a temperature range from 15-80 °C ...
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bioRxiv - Genetics 2024Quote: ... purified proteins were labeled with His-Tag labeling kit RED-tris-NTA following the manufacturer’s instructions (Nanotemper technologies), and 50 ng of labelled proteins were mixed with serial dilution of the lipids of interest and loaded into MST standard capillaries ...
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bioRxiv - Molecular Biology 2021Quote: ... The onset of scattering and melting temperatures based on the change of intrinsic protein fluorescence (ratio 350 nm/330 nm) were calculated in PR.ThermControl software (NanoTemper).
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bioRxiv - Biophysics 2020Quote: hGH was labelled with NT-647-NHS88 using the Monolith NT™ Protein Labelling Kit RED-NHS (NanoTemper Technologies) for 1 h at room temperature with NT-647-NHS at a molar ratio of 1:3 in labelling buffer following the protocol ...
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bioRxiv - Biochemistry 2020Quote: Purified His6-GlnA1 was fluorescently labeled using the Monolith Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper, Munich, Germany) with buffer B (50 mM HEPES ...
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bioRxiv - Plant Biology 2021Quote: ... MBP-PDF2(START)L467P were labeled in 50 mM sodium phosphate buffer (pH 7.4) supplemented with 500 mM NaCl using Monolith Protein Labeling kit RED-MALEIMIDE (NanoTemper) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... W25 was fluorescently labeled with the Monolith Protein Labeling Kit RED – NHS 2nd Generation (MO-L011, NanoTemper Technologies GmbH) following the protocol established in the manual ...
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bioRxiv - Cell Biology 2021Quote: Recombinant His-tagged proteins were labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (NanoTemper, MO-L008). In all experiments ...
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bioRxiv - Molecular Biology 2022Quote: SiaQM in amphipol was labelled at a ratio of 0.8 dye / 1 protein following the protocol of the RED-NHS 2nd generation labelling kit (NanoTemper). Labelled SiaQM was held constant at 20 nM and SiaP was titrated at 16 concentrations starting at 2,900 μM and serially diluting 2-fold to 90 nM ...
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bioRxiv - Biophysics 2022Quote: 6.6 µM GyrA14 (in 1X PBS) was labeled using the RED-NHS Monolith™ Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The purified PDZ domains were covalently labelled using a fluorescent dye reactive on amine following the manufacture’s protocol (Protein Labelling Kit RED-NHS, Nanotemper). A serial dilution of the WT and the P71L mutant of the E protein PBM peptide was prepared in the buffer containing PBS tween 20 0.05% ...
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bioRxiv - Plant Biology 2022Quote: Protein sample preparation and measurements were performed according to the protocol from Monolith NT (NanoTemper Technologies GmbH, Munich, Germany), employing quality controls as outlined by Sedivy 202143 ...
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bioRxiv - Molecular Biology 2022Quote: ... we evaluated the interaction of recombinant PfPHB1 and PfPHB2 proteins using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). Thermophoretic movement of a fluorescently labelled protein is measured by monitoring the fluorescence distribution ...
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bioRxiv - Systems Biology 2023Quote: ... NSP10 protein was fluorescently labeled using N-Hydroxysuccinimide (NHS)-ester fluorophores according to the manufacturer’s instructions (Protein Labeling Kit RED-NHS 2nd Generation, Nanotemper). Labeling was performed in the NSP16 protein storage buffer (20 mM HEPES pH 7.5 and 0.3 M NaCl) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were labelled in NHS labelling buffer using the Red NHS 2nd generation amine labelling kit (Nanotemper, MO-L011), then buffer exchanged into 10 mM potassium phosphate buffer ...
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bioRxiv - Bioengineering 2024Quote: Purified proteins after SEC were concentrated to 0.5-1 mg/mL for NanoDSF using a Prometheus NT.48 (Nanotemper). Capillaries were filled up with ∼ 15 µL of protein for melting scans between 20-95 °C with a pre- stabilization phase of 1 min and a temperature slope of 2 °C/min (37 min ...
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bioRxiv - Biochemistry 2024Quote: ... concentrated to 1 µM and lysine labeling was performed using the Red-NHS 2nd Generation Protein Labeling Kit (Nanotemper) according to manufacturer instructions ...
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bioRxiv - Genetics 2024Quote: Recombinant IRF6 protein was labeled using the Monolith His-Tag Labeling Kit RED-tris-NTA (Nanotemper Technologies, MO-L018) according to the manufacturer’s protocol at a 1:2 dye:protein ratio ...
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bioRxiv - Microbiology 2021Quote: ... The labelling of NyxA was performed by covalent coupling of the lysines of the protein to a fluorophore using the RED-NHS (Nanotemper) Protein Labeling Kit ...
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bioRxiv - Biochemistry 2020Quote: ... This FSS-PscN solution was then labeled with the Monolith Protein Labeling Kit RED-NHS (MO-L001) following the manufacturer instruction (Nanotemper) and diluted in the purification binding buffer (see above ...
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bioRxiv - Biophysics 2021Quote: The thermal and chemical denaturation of the WT and the mutant 14-3-3 proteins were performed using nano-DSF (Prometheus NT.48) from Nanotemper Technologies (Munchen ...
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bioRxiv - Microbiology 2022Quote: The purified HBsAg was purchased from ProSpec (hbs-872-c) and was labeled by RED-NHS dye (monolith protein labeling kit, MO-L011, NanoTemper) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: NanoDSF Systems were conducted to measure the thermostability and aggregation of purified protein using Prometheus NT.48 instrument (NanoTemper Technologies). A 10 μL aliquot of sample diluted to a concentration of 5 mg/mL were applied to quartz capillary cassette and placed into card slot ...
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bioRxiv - Biochemistry 2021Quote: C-terminally His6-tagged proteins were labeled using the Monolith His-tag labeling kit RED-tris-NTA (Nanotemper #MO-L008) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... His-Cdc42 was labeled following the instructions of the MO-L001 Monolith NT Protein Labeling Kit RED – NHS (NanoTemper Technologies). Labelled protein concentration in the binding reactions was 10 nM while compounds concentration was either 50 or 100 μM ...
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bioRxiv - Biochemistry 2022Quote: The amine residues of purified KsgA were fluorescently labeled with NHS red using the Protein Labeling Kit RED-NHS 2nd Generation (Cat # MO-L011 Nanotemper). The labeling reaction was performed according to the manufacturer’s protocol by mixing KsgA at a final concentration of 20 μM with a 3-fold molar excess of dye at room temperature for 30 minutes in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The TPR-CHAT and its mutants were fluorescence-labeled using the Protein Labeling Kit RED-NHS 2nd Generation (NanoTemper Technologies) at 10 μM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies with an excitation wavelength of 280 nm ...
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bioRxiv - Neuroscience 2024Quote: We followed the manufacturer’s instructions to label APOE3 and APOE4 using the Monolith protein labeling kit RED-NHS 2nd generation (NanoTemper Technologies). In summary ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Monitoring of the ITF at 330 nm and 350 nm during protein thermal denaturation was carried out in a Prometheus NT.48 instrument from NanoTemper Technologies (Munchen ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μl of a 1 mg/ml HiSiaP protein solution was prepared and 10 μl was soaked into a glass capillary (standard capillary, NanoTemper). The start and end temperature were set to 20 °C and 90 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing varying concentrations of protein were loaded into glass capillaries and applied to the nanoDSF device Prometheus NT.48 (Nanotemper). The samples were heated from 20 to 90°C with a slope of 1.5°C/min and the unfolding of the proteins was observed by detecting shifts in the fluorescence at 330 and 350 nm ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ColG-variants were labeled using the Monolith NT™ Protein Labeling Kit RED-NHS 2nd Generation Amine reactive (NanoTemper, Germany) and the yield of labelled protein and the degree of labelling were determined according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 µL of 100 nM solution of his-tagged protein was labeled with RED-tris-NTA 2nd Generation dye (Monolith HIS-tag labeling Kit, NanoTemper). Protein-protein complexes were obtained by mixing dye-labeled IFIT protein (final concentration 50 nM ...
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bioRxiv - Immunology 2024Quote: The thermal stability and unfolding of NA proteins were measured using the nanoDSF (differential scanning fluorimetry) technique on a Prometheus Panta instrument (Nanotemper). NanoDSF monitors changes in the intrinsic tryptophan or tyrosine fluorescence resulting from alterations in the 3D structure of proteins as a function of temperature ...
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bioRxiv - Biochemistry 2024Quote: Purified proteins as indicated were analysed by nano differential scanning fluorometry to assess thermal protein stability using a Tycho NT.6 (Nanotemper) with 10 µl capillaries ...
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bioRxiv - Immunology 2022Quote: ... Germany) and MST-Red-MIF was prepared using the Monolith Protein Labeling Kit RED-NHS 2nd Generation from NanoTemper (Munich, Germany), following the manufacturers’ instructions ...
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bioRxiv - Biophysics 2022Quote: Thermal scans with measurement of the intrinsic fluorescence of the protein samples were carried out using a Tycho instrument (Nanotemper, Germany). 10 μL samples were loaded in capillaries (TY-C001 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL of refolded protein was loaded into standard capillaries and onto the capillary tray of a Prometheus NT.48 instrument (NanoTemper Technologies) and run using a 5.0 °C/min thermal ramp at 50% LED excitation ...
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bioRxiv - Biophysics 2022Quote: His-tagged NCTD was fluorescently labelled with NT-647 amine reactive dye from the Monolith NT Protein Labelling kit RED-NHS (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RBD was labeled with the RED-NHS 2nd generation Monolith Protein Labeling Kit according to the manufacturer’s instructions (Nanotemper; MO-L011). The fluorescent group was conjugated to RBD through primary amines ...
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bioRxiv - Biochemistry 2021Quote: 6xHis-eIF1 was labeled with a Cy5 fluorophore through non-covalent linkage to the His-tag moiety using the Monolith Protein Labeling Kit RED-tris-NTA 2nd generation (NanoTemper Technologies). 40S subunits (0.58 nM –1.2 μM ...
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bioRxiv - Biophysics 2020Quote: ... The protein concentration was additionally measured on ND-1000 spectrophotometer (NanoDrop®) and the protein quality was evaluated by nanoDSF using a Tycho (Nanotemper).