Labshake search
Citations for NanoTemper :
51 - 100 of 254 citations for Prestained Protein Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Samples were loaded into nanoDSF Grade Standard capillaries (Cat# PR-C002, NanoTemper Technologies) in duplicates and transferred to a Prometheus NT.48 instrument (NanoTemper Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... using standard or premium capillaries on a Monolith NT.115TM MST machine (Nanotemper). Binding curves and Kinetic parameters were plotted and estimated using manufacturer provided software (NT Analysis 1.5.41 and MO ...
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bioRxiv - Biophysics 2020Quote: ... MST analysis was carried out using premium-coated and standard treated capillaries (NanoTemper), and buffer matching the SPR conditions (HEPES 10 mM ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were then loaded into standard capillaries and read on a NT.Automated (Nanotemper) using 10% excitation energy and “medium” MST power settings ...
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bioRxiv - Molecular Biology 2023Quote: ... 250 mM NaCl were loaded into nanoDSF Grade Standard Capillaries (NanoTemper, #PR-C002) and analysed on a Prometheus NT 48 instrument (Nanotemper) ...
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bioRxiv - Biophysics 2021Quote: ... Each dilution was filled into Monolith NT.115 standard treated capillaries (NanoTemper Technologies GmbH). Thermophoresis was measured using a Monolith NT.115 instrument ...
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bioRxiv - Microbiology 2021Quote: ... Samples were loaded in the Monolith NT.115 MST standard-treated capillaries (NanoTemper Technologies), mixed well quickly ...
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bioRxiv - Biophysics 2021Quote: ... Samples were run using Monolith NT.115 standard capillaries (Nanotemper Technologies, San Francisco, CA). The data were collected at the high excitation setting with the laser power set at 20% ...
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bioRxiv - Molecular Biology 2022Quote: ... All interactions were measured in Monolith NT.115 Standard Treated Capillaries (MO-K002, Nanotemper) at 25℃ with an MST-on time of 20 seconds and an MST-off time of 5 seconds ...
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bioRxiv - Biochemistry 2019Quote: MST was measured on 10 μl reactions using standard capillaries (MO-K022, NanoTemper Technologies) on a Monolith NT.115 (NanoTemper Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... Tubes were shifted to RT and mixtures were transferred to 16 standard capillaries (Nanotemper, Germany) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2019Quote: Microscale thermophoresis was carried out in standard capillaries (Cat# MO-K022, Nanotemper Technologies, Munich, Germany) on a Monolith NT.115Pico instrument (Nanotemper Technologies ...
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bioRxiv - Immunology 2024Quote: ... Each 20 μL sample was split between two Prometheus Grade Standard Capillaries (Nanotemper, Munich, Germany) and placed in the capillary tray of a Prometheus Panta (Nanotemper ...
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bioRxiv - Biochemistry 2022Quote: ... All measurements were carried out at room temperature in standard capillaries (Nanotemper Technologies, Cat# MO-K022) using 20% excitation power and 20% MST power ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 30 min before loading into standard glass capillaries (MO-KO22, NanoTemper Technologies). MST measurements were performed using a Monolith NT.115 (NanoTemper Technologies ...
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bioRxiv - Microbiology 2022Quote: ... AtExo70B1 and their complex were loaded in nanoDSF grade standard capillaries (NanoTemper Technologies GmbH, München, Germany) and exposed at thermal stress from 20 ⍰C to 95 ⍰C by thermal ramping rate of 1 aC/min ...
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bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were loaded by capillary action into standard sensitivity capillaries and placed within a Prometheus Panta (Nanotemper). The instrument was allowed to stabilize at 20°C before measuring ...
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bioRxiv - Molecular Biology 2022Quote: ... 20% LED power in standard capillaries at 25°C on a Monolith NT.115 device (NanoTemper Technologies). The data was analyzed using the MO.affinity analysis software (V2.3 ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... Standard capillaries were filled with the samples for measurements with NanoTemper Monolith NT.115 instrument (NanoTemper, Germany). The measurement was performed in standard capillaries (NanoTemper ...
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bioRxiv - Biochemistry 2024Quote: ... Then all samples were loaded into Prometheus NT.48 nanoDSF Grade Standard Capillaries (PR-C002, NanoTemper Technologies), the fluorescence signal was recorded under a temperature gradient ranging from 25 to 90◦C (flow rate of 0.5◦C·min−1) ...
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bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: MST assays were performed in a Monolith NT.115 using Standard Treated Glass Capillaries (NanoTemper Technologies MO-K002). His-TEV-UCH37(C88S)-RPN13C was labelled with Monolith His-Tag labeling Kit RED-tris-NTA (MO-L008 ...
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bioRxiv - Molecular Biology 2020Quote: ... After incubation the samples were quickly spun down and loaded into Monolith NT.115 Standard capillaries (NanoTemper Technologies) at room temperature (~25 °C) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting mixtures were loaded into a Monolith NT.115 series standard capillaries (NanoTemper, catalogue no. MO-K002). Thermophoresis experiments were performed with 40% of MST power and 20% of LED power for fluorescence acquisition.
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bioRxiv - Molecular Biology 2023Quote: ... The mixtures were incubated at room temperature for 10 min and transferred to 16 standard capillaries (Nanotemper, Germany). Data were acquired using medium MST power ...
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bioRxiv - Bioengineering 2023Quote: ... Then 20 μL sample was loaded into standard treated capillaries for measurements on a Monolith NT Automated (Nanotemper).
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bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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bioRxiv - Systems Biology 2021Quote: ... The sample was then loaded into standard treated capillaries and analyzed using a Monolith NT.115 (NanoTemper Technologies Germany). The blue laser was used for a duration of 35 s for excitation (MST power = 60% ...
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bioRxiv - Molecular Biology 2022Quote: ... Around 12 μL of protein complex at a concentration of 0.15 mg/mL in buffer B were applied into standard grade NanoDSF glass capillaries (Nanotemper). Capillaries were loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample was measured with two technical and three independent biological replicates in standard capillaries (Nanotemper Technologies, Munich, Germany) with the Prometheus device (Nanotemper Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... The samples (10 µL at 0.2 mg/mL in HBS) were loaded into standard treated NT.115 capillaries (NanoTemper), and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper ...
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bioRxiv - Biochemistry 2019Quote: Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
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bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
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bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
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bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Fluorescently labeled 100 nM NKR-P1 was mixed with dilution series of LLT1 or LLT1SIM and analyzed in standard capillaries in an NT.115 Monolith (NanoTemper) using 30% LED excitation power and 60% MST power ...
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bioRxiv - Biochemistry 2022Quote: ... Measurements were done in triplicate at multiple concentrations (50 µM, 5 µM, and 0.5 µM) in Tycho standard capillaries (Nanotemper Technologies). Data analysis was performed via MO Affinity Analysis Software v2.1.3 (Nanotemper Technologies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and then loaded into 3 standard capillaries for triplicate readings in a Prometheus NT.48 instrument (Nanotemper Technologies, Munich, Germany). Experiments were run using 40% excitation power ...
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bioRxiv - Biophysics 2021Quote: ... Protein samples with a concentration ranging between 0.5 and 1.0 mg/ml were measured in triplicates in quartz capillaries (Prometheus NT.48 Series nanoDSF grade standard capillaries, NanoTemper Technologies). Fluorescence measurements were made with an excitation wavelength of 280 nm ...
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bioRxiv - Biophysics 2019Quote: ... Nucleoprotein samples in PBS buffer solution (at concentrations of 3 mg/ml) were loaded into quartz capillaries (Standard Capillaries Prometheus NT.48, NanoTemper); capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biophysics 2020Quote: Proteins mixed to a final concentration of 30 μM in presence of 200 μM PIP2 and incubated overnight at 4 °C were used to fill two standard grade NanoDSF capillaries (Nanotemper) and loaded into a Prometheus NT.48 device (Nanotemper ...
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bioRxiv - Biophysics 2022Quote: ... HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml) were collected in glass capillaries (Standard Capillaries Prometheus NT.48, NanoTemper), capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biophysics 2023Quote: ... 0.2 mM ATP) with 0.05 % Tween-20 on a Monolith NT.115 Microscale Thermophoresis (MST) device using standard treated capillaries (NanoTemper Technologies). Actin (final labelled-actin concentrations adjusted to 60 nM ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
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bioRxiv - Physiology 2023Quote: ... Purified OsABG protein was labeled using the protein labeling kit Red-NHS 2nd generation (MO-L011, Nanotemper) which dyes primary lysine residues in the protein ...