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Citations for NanoTemper :
51 - 100 of 230 citations for Fatty acid binding protein FABP3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Binding assays were performed by electrophoretic mobility shift assay (EMSA) and Microscale thermophoresis (MST, NanoTemper Munich). Dilution series (1.5 fold ...
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bioRxiv - Molecular Biology 2023Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
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bioRxiv - Biochemistry 2023Quote: ... binding affinities were determined via microscale thermophoresis on a Monolith NT.115 instrument (NanoTemper Technologies, Germany). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
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bioRxiv - Biochemistry 2019Quote: ... was labeled with Monolith His-Tag Labeling Kit RED-tris-NTA kit (NanoTemper Technologies, München, Germany). Briefly ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... by staining His-tagged xtZnT8 with the RED-Tris-NTA 2nd Generation dye (NanoTemper Technologies, Germany). For Zn2+ titration ...
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bioRxiv - Physiology 2024Quote: ... cMyBP-C constructs were fluorescently labeled via His-tag using a commercially available kit (NanoTemper Technologies). Unlabeled sS1 or 15-hep myosin was titrated against 84 nM fluorescently labeled C3 or C0C7 protein ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled using the Monolith His-Tag Labeling Kit RED-Tris-NTA second generation (NanoTemper Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Fluorescence values from the binding reactions were determined using the Monolith NT.115 (NanoTemper Technologies, München, Germany). Binding data was analyzed using GraphPad Prism (GraphPad Software ...
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bioRxiv - Biochemistry 2022Quote: ... The binding affinity measurements were carried out using the modified manufacturer’s protocol (Monolith NT.115pico, NanoTemper Technologies) in the Center for Macromolecular Interactions in Harvard Medical School (Boston ...
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bioRxiv - Molecular Biology 2023Quote: ... Each binding experiment was performed in three independent runs and resulting MST data loaded to MO.Analysis (NanoTemper) software to estimate the Kd for MRE11 dimerization ...
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bioRxiv - Biochemistry 2022Quote: His6-tagged caspases were labelled using the His-tag labelling Kit RED-tris-NTA 2nd generation (NanoTemper). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... as recommended by the Monolith His-Tag Labeling Kit RED-tris-NTA protocol (NanoTemper Technologies, MO-L008). The binding buffer used for reactions [50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... were fluorescently labeled using His-Tag Labeling Kit RED-Tris-NTA 2nd Generation (NanoTemper, Cat# MO-L018). The proteins (at fixed concentration ...
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bioRxiv - Molecular Biology 2022Quote: The kinetic measurements of PfCoSP-gDNA binding were conducted using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001 ...
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bioRxiv - Biochemistry 2023Quote: Binding experiments were performed using microscale thermophoresis (MST).39 Experiments were performed on a Monolith NT.115 (NanoTemper) using 20% light-emitting diode power ...
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bioRxiv - Biochemistry 2019Quote: ... C0-C7 was fluorescently labeled with a commercial His-tag labeling kit according to manufacturer instructions (Nanotemper technologies). His-labeled C0-C7 fluorescence was measured by a red LED at 60-90% excitation power (RED filter ...
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bioRxiv - Molecular Biology 2020Quote: ... We measured the fluorescence signal from Gαi1β1γ1 by using the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies). The samples were prepared in a buffer containing 20 mM sodium phosphate (pH 6.9) ...
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bioRxiv - Biochemistry 2019Quote: ... was labelled with 100 μM Monolith NTTM His-Tag Labeling kit RED-tris-NTA (L008, NANOTEMPER, Munich, Germany) 30 min at room temperature (RT ...
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bioRxiv - Plant Biology 2022Quote: ... The N-terminal His-tag was labelled with the RED-tris-NTA kit (NanoTemper Technologies GmbH, Munich, Germany). After 10 minutes incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... + 0.5% DDM and was labeled using a His-Tag Labeling Kit RED-tris-NTA 2nd Generation Kit (NanoTemper) via the manufacturers protocol in 1.7 ml low-adhesion tubes ...
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bioRxiv - Biophysics 2021Quote: Thermal stability and binding affinity were determined using nanoscale differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper Technologies). Capillaries were filled with 10 μL of respective samples in duplicates and loaded into the reading chamber ...
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bioRxiv - Microbiology 2023Quote: Nucleotide binding assays based on microscale thermophoresis were performed with a Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany) using Monolith NT Premium Capillaries ...
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bioRxiv - Biophysics 2021Quote: 6xHis-RAD51 was labelled with the Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation kit (NanoTemper Technologies), which specifically recognizes the hexahistidine tail of the recombinant protein.
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bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
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bioRxiv - Biophysics 2020Quote: The binding affinity of spike to Sb23 was measured by microscale thermophoresis using the MST NT.115 device (NanoTemper Technologies). Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Signal to noise ratio was used to evaluate the quality of the binding data according to NanoPedia instructions (NanoTemper Technologies). Only a signal-to-noise ratio of more than 5 was considered acceptable while a signal to noise of more than 12 was considered excellent.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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bioRxiv - Cell Biology 2021Quote: Binding of cevipabulin to the seventh site was detected with an MST assay with a Monolith NT.115 instrument (NanoTemper Technologies). Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The best data quality was obtained at 90% MST power which was thus used for quantifying the binding affinities using the MO.Affinity Analysis software (NanoTemper Technologies, Germany).
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bioRxiv - Biochemistry 2023Quote: Microscale thermophoresis (MST) analysis was carried out to determine the binding affinity between hexon and hLF using Monolith NT.115 (Nanotemper Technologies). Hexon was labelled with AlexaFluor594 (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 30 min with 50 nM RED-tris-NTA 2nd Generation dye from the Monolith His-Tag Labeling Kit (Cat.#MO-L018; NanoTemper Technologies). The labeling reaction mixture was centrifuged for 10 min at 4°C and 15,000 x g to remove protein aggregates ...
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bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: MST experiments to measure the binding affinities between TadAs and the 19-nt DNA substrate were performed on a Monolith NT.115 system (NanoTemper Technologies, Germany) using the nano BLUE detector ...
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bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018, NanoTemper Technologies, GmbH, Munich, Germany) per the manufacturer’s instructions with slight modifications (43) ...
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bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
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bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: We measured the binding affinity of MTBDs (fluorescently labeled target) to microtubules (unlabeled ligand) using a Monolith NT.115 MST system (NanoTemper Technologies, GmbH, Munich, Germany). We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018 ...
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bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
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bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...
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bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
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bioRxiv - Microbiology 2022Quote: ... Protein-protein interactions were analyzed at 20°C by MST on a Monolith NT.115 (NanoTemper technologies) using standard capillaries ...
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bioRxiv - Physiology 2023Quote: ... Purified OsABG protein was labeled using the protein labeling kit Red-NHS 2nd generation (MO-L011, Nanotemper) which dyes primary lysine residues in the protein ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
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bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Phr2 and Pga4 proteins and Bull Serum Albumin (BSA) were labeled using the Monolith™ RED-NHS Protein Labeling Kit (NanoTemper Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...