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Citations for EpiCypher :
1 - 50 of 57 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and 1 μl E-Coli spike-in DNA (0.5 ng/μl) (EpiCypher) to each tube ...
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bioRxiv - Cell Biology 2023Quote: ... Normalization of sample inputs was performed when applicable either using Spike-in (either from reads from E Coli genome or barcode reads from the K-MetStat panel (EpiCypher 19-1002)) in accordance with manufacturer’s instructions or CPM normalization to total reads when applicable ...
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bioRxiv - Neuroscience 2023Quote: ... coli Spike-in DNA (EpiCypher) was added to each sample to normalise reads to control for experimental variability and sequencing depth ...
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bioRxiv - Neuroscience 2022Quote: ... coli spike-in / sample (Epicypher #18-1401) mixture was added to each sample ...
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bioRxiv - Genomics 2023Quote: ... coli spike-in DNA (Epicypher, 18-1401) was added at 0.01 ng per reaction ...
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bioRxiv - Biochemistry 2023Quote: ... coli Spike-in DNA (EpiCypher 14-1048). After incubation for 10 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... coli DNA Spike-in (EpiCypher, 18-1401)) and incubate at 37 °C for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... coli spike-in DNA (EpiCypher, #18-1401) were added and incubated for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... coli DNA Spike-in (EpiCypher, Cat #18-1401) and incubated at 37 °C for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... coli Spike-In DNA (provided with the EpiCypher kit) into the STOP buffer for quenching pAG-MNase activity ...
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bioRxiv - Biochemistry 2022Quote: ... coli (ISWI, ModT antigen and LCMS reference proteins) or obtained from EpiCypher Inc ...
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bioRxiv - Genomics 2021Quote: CUT&Tag was performed using CUTANA™ CUT&Tag reagents (Epicypher, Durham NC ...
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bioRxiv - Genomics 2021Quote: ... then the beads were resuspended in pAG-Tn5 pre-loaded with mosaic-end adapters (Epicypher cat. no. 15-1117 1:20) in 300-wash buffer (Wash buffer except containing 300 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: CUT&TAG was conducted based on the CUTANA CUT&TAG protocol v1.5 (Epicypher). Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg ...
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bioRxiv - Cancer Biology 2024Quote: ... This incubation/wash cycle was repeated for the guinea pig anti-rabbit secondary antibody (Antibodies Online cat. no. ABIN101961) and for pAG-Tn5 preloaded with mosaic end adapters (Epicypher cat. no. 15-1117 1:20), followed by a Triton-Wash rinse and transfer of the slide to 10 mM TAPS pH 8.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... Coli DNA (Epicypher cat# 18-1401) at 5.25 pg per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μg/mL Glycogen with E.coli spike in control (Epicypher, 18-1401)).
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bioRxiv - Genomics 2022Quote: ... and pAG-Tn5 for CUT&Tag (EpiCypher, SKU: 15-1017), with the following exceptions ...
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bioRxiv - Cancer Biology 2023Quote: ... The CUT&Tag experiment was performed according to the CUT&Tag Protocol (v1.7) as previously described by EpiCypher (derived from Kaya-Okur et al.76). In brief ...
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bioRxiv - Developmental Biology 2023Quote: ... a 1:5 dilution of K-MetStat Panel spike-in nucleosomes (EpiCypher, cat. # 19-1002) was added to each reaction prior to addition of antibody ...
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bioRxiv - Genomics 2021Quote: We followed the CUTANA Direct-to-PCR CUT&Tag protocol (EpiCypher) to generate two replicates of low-background CUT&Tag libraries ...
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bioRxiv - Genomics 2022Quote: ... CUT&Tag reactions were performed using Epicypher reagents (protocol v1.5; Epicypher, USA). Briefly ...
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bioRxiv - Genomics 2022Quote: In order to align reads originated from the spike-in control (SNAP-ChIP K-MetStat Panel, Epicypher), we combined PacBio genomes with DNA sequence barcode of the SNAP-ChIP K-MetStat Panel ...
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bioRxiv - Molecular Biology 2022Quote: Cut & Tag experiments were performed in duplicate using H3K4me3 antibody 13-0041 (EpiCypher) and H3K27ac antibody ab4729 (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rabbit antibody for CUTANA™ CUT&Tag Workflows (13-0047, EpiCypher).
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bioRxiv - Genetics 2020Quote: ... Another modification was to spike-in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher, SKU: 19-1001) (34) ...
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bioRxiv - Molecular Biology 2023Quote: Genome-wide H3K27me3 domain changes were profiled using the CUT&Tag protocol adapted from EpiCypher. Cells were harvested via 0.25% trypsin-EDTA solution and counted using 0.4% trypan blue stain and Countess automated cell counter ...
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bioRxiv - Developmental Biology 2023Quote: Differential SMARCC1 binding between E11.5 (40-43s) control (Gli3+/+; n=3) and Gli3-/- (n=4) samples was conducted using an E.coli spike-in (EpiCypher 18-1401) of 0.125ng/sample ...
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bioRxiv - Neuroscience 2022Quote: CUT&Tag library construction was performed using CUTANA™ High Fidelity 2X PCR Master Mix (15-1018, EpiCypher) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN was performed with anti-HA antibody (HA Tag CUTANA, 13-2010) and a commercial kit (EpiCypher CUTANA pAG-MNase for ChIC/CUT&RUN ...
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bioRxiv - Developmental Biology 2022Quote: The Cleavage Under Targets & Tagmentation (CUT&Tag) experiments (58) were performed using reagents and following a protocol from EpiCypher. For Atoh7 and Pou4f2 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... (a step-by-step protocol https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3) using CUTANA™ pAG-Tn5 (Epicypher, 15-1017). Quantitative spike-in CUT&Tag was performed by adding Drosophila S2 cells at a 2:1 ratio to mouse spermatogonial cells (20,000 S2 cells to 10,000 mouse cells ...
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bioRxiv - Genetics 2022Quote: ... containing 150 ng of DNA for leaf or 350 ng for ear was then diluted to a total volume of 1500 μL in 1X Wash Buffer and used as input for the CUT&Tag-IT Assay Kit with both anti-CENH3 (maize) antibodies (26) and IgG for each sample (Epicypher 13-0042). Libraries were amplified with 15 or 16 cycles of PCR ...
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bioRxiv - Biophysics 2020Quote: ... Histone proteins were purchased from EpiCypher. While the H3 histones were available as part of recombinant human histone octamers ...
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bioRxiv - Cancer Biology 2023Quote: ... All subsequent steps through to library preparation and purification f ollowed t he standard CUT&Tag-direct protocol (19) using pAG-Tn5 (Epicypher cat. no. 15-1117), except that 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL i5 universal primer (EpiCypher), 2 µL i7 barcoded primer (EpiCypher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL i7 barcoded primer (EpiCypher), 0.005 ng Escherichia coli spike-in DNA (made in-house) ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein A-micrococcal nuclease (pA-MN; EpiCypher donation) was added to a concentration of 1:400 to nuclei ...
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bioRxiv - Developmental Biology 2023Quote: ... we used protein A/G-MNase from Epicypher. For 24h pupal samples with 40 and 75 wings ...
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bioRxiv - Genomics 2023Quote: ... The following commercial loaded protein A-Tn5 transposase fusion protein (pA-Tn5) were used at recommended dilutions by the manufacturer: CUTANA™ pAG-Tn5 (Epicypher 15-1017; Lot 20142001-C1), or pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins (0.4 μM) were mixed with nucleosome (50N66, 0.03 μM - Epicypher) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). Nucleosome reconstitutions were performed by salt gradient dialysis64 ...
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bioRxiv - Biophysics 2024Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). We followed the previously published protocol to prepare nucleosome reconstitutions [80] ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µl of SNAP-ChIP K-MetStat panel and K-AcylStat panel nucleosomes (EpiCypher) respectively ...
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bioRxiv - Genomics 2020Quote: ... The pAG-Tn5 fusion protein used in many of these experiments was a gift from Epicypher, Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
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bioRxiv - Immunology 2021Quote: ... This was followed by washing and incubation with the protein A/G conjugated Tn5 (CUTANA pAG-Tn5, Epicypher) enzyme for one hour at room temperature ...