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Citations for EpiCypher :
1 - 50 of 52 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Plant Biology 2024Quote: ... recombinant mononucleosomes (EpiCypher) as substrates ...
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bioRxiv - Molecular Biology 2023Quote: One μg of recombinant (Epicypher) or cellular nucleosomes (see protein purification above ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed twice more before incubation with recombinant p-AG MNase (Epicypher 15-1016) at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... One ug of recombinant designer demethylated nucleosomes (EpiCypher) was incubated with the PHF21A-containing complexes from 293T cells ...
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bioRxiv - Cell Biology 2021Quote: Recombinant semi-synthetic designer nucleosomes (dNucs) were from EpiCypher. Two phases of dCypher® testing on the PerkinElmer AlphaScreen® platform were performed as previously described9 ...
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bioRxiv - Cell Biology 2019Quote: ... 15 uL of recombinant nucleosomes (SNAP-ChIP, EpiCypher, 19-1001) were fixed with fresh 1% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004), and 1 μCi of 3H-SAM (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
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bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...
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bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Biochemistry 2022Quote: ... Experiments done with excess ubiquitinated nucleosome used purified recombinant mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-K120Ub) (EpiCypher, 16-0370) at 400 nM and 25 nM SAGA ...
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bioRxiv - Immunology 2021Quote: ... nuclei were isolated from 100,000 primary human macrophages and bound to activated magnetic concanavalin A beads (Epicypher). The bound nuclei were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cell-bead antibody complex was then washed with cell permeabilization buffer and incubated with pAG-MNase (EpiCypher), followed by addition of 2 mM CaCl2 and incubation for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with pAG-MNase (Epicypher) in dig wash buffer then in reaction buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated with pAG-MNase (EpiCypher) for 10 minutes at room temperature and washed with Digitonin Buffer ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies (FOXP1 Cell Signaling Technologies #2005S, FOXP2 Abcam #ab16046, FOXP4 Millipore #ABE74, H3K27me3 Cell Signaling Technologies #9733, IgG Epicypher #13-0042) were added at 1:50 dilution and samples were nutated overnight at 4C.
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bioRxiv - Genomics 2020Quote: ... cells were incubated with CUTANA pA/G-MNase (Epicypher) and targeted chromatin digestion initiated by the addition of 100 mM CaCl2 and allowed to proceed for 2 hours at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... approximately cells were adsorbed to concanavalin A beads (Epicypher), and washed (150mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were incubated with pAG-MNase (EpiCypher, #15-1016) for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cell pellets were incubated with pA/G-MNase (20X) enzyme (EpiCypher) in 50µl buffer 1 at 4°C for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were incubated on activated Concanavalin A conjugated paramagnetic beads (EpiCypher) at room temperature for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed again and incubated with CUTANA™ pAG-MNase (Epicypher) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... About 900’000 cells were processed using the CUTANA kit (Epicypher, #14-1048). Briefly ...
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bioRxiv - Genomics 2022Quote: ... 500K permeabilized cells were immobilized onto Concanavalin-A beads (Con-A; EpiCypher #21-1401) and incubated overnight (4° C with gentle rocking ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041 ...
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bioRxiv - Molecular Biology 2023Quote: ... CX3CR1Pos cells were subjected to CUT&RUN using the CUTANA™ ChIC/CUT&RUN kit (Epicypher) using an anti-FLAG antibody (Sigma M2 #1804 ...
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bioRxiv - Molecular Biology 2024Quote: ... Bead-bound cells were resuspended in Dig-wash buffer containing 1x CUTANA™ pAG-MNase (Epicypher) and rotated for 1 hr at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell-bead slurry was washed and incubated with pAG-MNase (1:20 dilution, EpiCypher, 15-1116) for 10 min at RT ...
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bioRxiv - Genetics 2023Quote: ... 2 μl /1×106 cells of the following antibodies were used for immunoprecipitations: H3K4me3 (EpiCypher 13-00041), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Genetics 2023Quote: ... Bead-bound cells were resuspended in 50 μl secondary antibody buffer (goat anti-rabbit IgG (Epicypher, 13-0047) diluted 1:100 in 20 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts, Epicypher Cat# 13-0031), Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns ...
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bioRxiv - Biochemistry 2022Quote: Chromatin profiling for WT and ACK KO K562 cells was performed using CUTANA™ ChIC/CUT&RUN Kit (EpiCypher) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell and bead suspension was incubated in buffer containing 0.05% digitonin and 0.5ug of anti-HA (Epicypher 13-2010) antibody overnight at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... an input of ∼400,000 cells per antibody condition and an overnight antibody incubation at 4°C with H3K4me3 (0.5 μg/ml, EpiCypher), H3K9me3 (0.9 μg/ml ...
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bioRxiv - Genomics 2024Quote: ... Cut&Run each sample used 300,000 cells and included a modified wash buffer and fixation steps adapted from CUTANA Cross-linking Protocol v1.9 from Epicypher. Samples were fixed with either 0.1 or 1% fresh paraformaldehyde for 2 min and quenched with 2.5M Glycine at 1:20 dilution before proceeding to wash steps ...
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bioRxiv - Cancer Biology 2024Quote: A total of 500,000 cells per reaction were harvested and captured using 10 µL of pre-activated ConA beads (EpiCypher). Beads with attached cells were incubated at room temperature for 10 min to ensure complete adsorption ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell-bead slurry was washed twice with Digitonin Buffer and incubated with 1x Protein-A/G-MNase (pAG-MNase; Epicypher) in Digitonin Buffer for 10 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... We used immuno-tethered CUT&Tag chromatin profiling [Kaya-Okur et al, 2020] with antibodies to histone H3K27me3 (C36B11, Cell Signalling Technology) and to histone H3K4me2 (13-0027, Epicypher) modifications ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated cells were counted in haemocytometer and approximately 250,000 cells/sample were used for further processing using CUTANA ChIC/ CUT & RUN kit (Epicypher, 14-1048) according to the manufacture’s protocol ...
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bioRxiv - Genomics 2022Quote: CUT&RUN was performed on in-vitro polarized Th1 cells using CUTANA™ ChIC/CUT&RUN Kit (EpiCypher, Cat#14-1048), using manufacturer’s recommendation ...