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Citations for EpiCypher :
1 - 50 of 63 citations for Mouse Fc Receptor Like Protein 1 FCRL1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
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bioRxiv - Biophysics 2020Quote: ... Histone proteins were purchased from EpiCypher. While the H3 histones were available as part of recombinant human histone octamers ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein A-micrococcal nuclease (pA-MN; EpiCypher donation) was added to a concentration of 1:400 to nuclei ...
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bioRxiv - Developmental Biology 2023Quote: ... we used protein A/G-MNase from Epicypher. For 24h pupal samples with 40 and 75 wings ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5μg of rabbit glucocorticoid receptor antibody [abcam, ab225886, 1:20] or rabbit IgG negative control antibody [Epicypher, 13-0041k, 1:50]). The remaining steps were performed according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The following commercial loaded protein A-Tn5 transposase fusion protein (pA-Tn5) were used at recommended dilutions by the manufacturer: CUTANA™ pAG-Tn5 (Epicypher 15-1017; Lot 20142001-C1), or pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins (0.4 μM) were mixed with nucleosome (50N66, 0.03 μM - Epicypher) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). Nucleosome reconstitutions were performed by salt gradient dialysis64 ...
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bioRxiv - Biochemistry 2022Quote: ... coli (ISWI, ModT antigen and LCMS reference proteins) or obtained from EpiCypher Inc ...
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bioRxiv - Biophysics 2024Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). We followed the previously published protocol to prepare nucleosome reconstitutions [80] ...
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bioRxiv - Genomics 2020Quote: ... The pAG-Tn5 fusion protein used in many of these experiments was a gift from Epicypher, Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... This was followed by washing and incubation with the protein A/G conjugated Tn5 (CUTANA pAG-Tn5, Epicypher) enzyme for one hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell-bead slurry was washed twice with Digitonin Buffer and incubated with 1x Protein-A/G-MNase (pAG-MNase; Epicypher) in Digitonin Buffer for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed in Wash buffer 300 (Wash buffer containing 300mM NaCl) two times and resuspended in Wash buffer 300 containing Protein A/G-TN5 (EpiCypher) and incubated for an hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... For negative control samples (e.g. rabbit α-mouse IgG) as well as H3K27me3 samples 1 µL of SNAP-CUTANA K-MetStat Panel (Epicypher #19-1002) was added to each sample ...
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bioRxiv - Genomics 2021Quote: ... or goat anti-mouse (Epicypher #13-0048) polyclonal secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... H3K27Ac mouse monoclonal antibody (EpiCypher, # 13-0045), HIRA(34) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Digitonin and 0.5 μg of anti-rabbit or anti-mouse secondary antibodies (Epicypher). After incubation for 30 min at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041, 1:50], or rabbit IgG negative control antibody [Epicypher, 13-0042, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Genomics 2021Quote: ... and incubated for 1 hour at room temperature for the guinea pig anti-rabbit secondary antibody (Antibodies Online cat. no. ABIN101961 1:100) and for 1 hour at room temperature for pAG-Tn5 (Epicypher cat. no. 15-1117, 1:20) tethering ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:100 H3K4me3 Antibody (Epicypher, #13-0041), 1:50 H3K27me3 antibody (Cell Signaling ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts, Epicypher Cat# 13-0031), Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns ...
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bioRxiv - Biochemistry 2022Quote: ... 1 µg of anti-H3K27ac (EpiCypher, 13-0045), 0.6 µg of anti-Stat5 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-ASYM26 (13-0011, Epicypher, 1:1000). Immunofluorescence were performed with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... anti-BRG1 (13-2002, EpiCypher, 1:50 dilution), and non-immune rabbit IgG (2729 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-H3K4me3 (13-0041, EpiCypher, 1:50 dilution), anti-H3K27me3 (9733 ...
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bioRxiv - Biochemistry 2024Quote: ... rabbit anti-H3K9me1 (1:5000, Epicypher 13-0029), rabbit anti-H3K9me2 (1:5000 ...
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bioRxiv - Genetics 2019Quote: ... and H3(1-43) peptides were obtained from EpiCypher. Binding reactions consisting of protein (13 µg) ...
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bioRxiv - Genetics 2022Quote: ... and H3K4me3 (at 1:2,000 dilution; Epicypher, Cat#13-0041), followed by an anti-rabbit secondary antibody (used at 1:2,000 dilution) ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with H3 antibody (1:50,000; EpiCypher 13-0001) or H3K27me3 antibody (1:2,000 ...
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bioRxiv - Genomics 2023Quote: ... then 1:20 CUTANA™ pAG-Tn5 (Epicypher 15-1017), or 1:250 pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:100 Rabbit IgG Negative Control Antibody (Epicypher, #13-0042) and incubated overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to each sample (RELA antibody [Santa Cruz, sc-109, 1:25] or rabbit IgG negative control antibody [EpiCypher, 13-0041, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004), and 1 μCi of 3H-SAM (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... loaded with Illumina NEXTERA adapters (a kind gift from the Henikoff lab, via Madapura lab, diluted 1:250, or Epicypher CUTANA® pAG-Tn5, diluted 1:10) was prepared in Dig-300 Buffer (0.05% Digitonin ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μl E-Coli spike-in DNA (0.5 ng/μl) (EpiCypher) to each tube ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of Rabbit anti-H3K4me3 ChIP grade antibody (Epicypher, 13-0041), which has been SNAP-ChIP validated for H3K4me3 specificity 62 ...
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bioRxiv - Cell Biology 2023Quote: ... the following primary antibodies were used: anti-H3K9ac (rabbit, Epicypher 13-0033, 1:500), anti-γH2Av (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... the arrays were incubated with a 1:1000 dilution of anti-GST (EpiCypher, 13-0022) in 1% milk 1x PBST for two hours at 4oC with shaking ...
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bioRxiv - Developmental Biology 2023Quote: ... a 1:5 dilution of K-MetStat Panel spike-in nucleosomes (EpiCypher, cat. # 19-1002) was added to each reaction prior to addition of antibody ...
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bioRxiv - Genetics 2023Quote: ... or CUTANA™ CUT&RUN Library Prep Kit with Primer Set 1 (14-1001, EpiCypher), and library concentrations were quantified with the Qubit 1 X dsDNA HS Assay Kit (Q33231 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041 ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell-bead slurry was washed and incubated with pAG-MNase (1:20 dilution, EpiCypher, 15-1116) for 10 min at RT ...
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bioRxiv - Genetics 2023Quote: ... 2 μl /1×106 cells of the following antibodies were used for immunoprecipitations: H3K4me3 (EpiCypher 13-00041), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Genomics 2023Quote: ... resuspended in pAG-Tn5 pre-loaded with mosaicend adapters (EpiCypher cat. no. 15-1117, 1:20 dilution) in 300-Wash buffer (Wash buffer except containing 300 mM NaCl ...