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Citations for EpiCypher :
51 - 100 of 101 citations for Microtubule Associated Protein 1 Light Chain 3 Gamma MAP1LC3C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and antibody bound DNA was then enzymatically cut with pAG-MNase (Epicypher). Samples were treated with Stop Buffer (340mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: Cut & Tag experiments were performed in duplicate using H3K4me3 antibody 13-0041 (EpiCypher) and H3K27ac antibody ab4729 (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rabbit antibody for CUTANA™ CUT&Tag Workflows (13-0047, EpiCypher).
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted with the primary antibodies against ADMA (Epicypher, SKU: 13-0011), SDMA (Millipore Cat# 07-413 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell-bead slurry was washed twice with Digitonin Buffer and incubated with 1x Protein-A/G-MNase (pAG-MNase; Epicypher) in Digitonin Buffer for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed in Wash buffer 300 (Wash buffer containing 300mM NaCl) two times and resuspended in Wash buffer 300 containing Protein A/G-TN5 (EpiCypher) and incubated for an hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Cancer Biology 2021Quote: ... After overnight incubation at 4°C with the primary FOXA1 antibody (13-2001, Epicypher), cell-bead slurry was washed twice more after which pA-MNase digestion was activated by placing samples on an ice-cold block and incubated with digitonin wash buffer containing 2 mM CaCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% BSA) and 10 µL per antibody treatment of ConA-coated magnetic beads (Epicypher) added with gentle vortexing ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Digitonin and 0.5 μg of anti-rabbit or anti-mouse secondary antibodies (Epicypher). After incubation for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: All histone markers antibodies for CUT&RUN were either certified by EpiCypher (H3K4me1, H3K4me3, H3K27me3) or validated by SNAP-ChIP qPCR (H3K27Ac) ...
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bioRxiv - Cancer Biology 2023Quote: ... H3K27Ac antibody was incubated with SNAP-ChIP® K-AcylStat™ Panel (EpiCypher 19-3100) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized using 0.01% digitonin and incubated with 0.5μg antibody (anti-Sox9 (CST, #82630) and IgG (# 13-0042; Epicypher) in 50μL antibody buffer (20mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... & protease inhibitors) followed by the addition of 0.5 µg CUTANA anti-rabbit secondary antibody (#13-1047, EpiCypher). Samples were then incubated on nutator at room temperature for 1 hour to facilitate binding ...
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bioRxiv - Genetics 2023Quote: ... Heterochromatin formation or gene inactivation were detected using antibodies against H3K4me3 (0.5 µg/sample, EpiCypher #13-0041), H2K27me3 (0.5 µg/sample ...
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bioRxiv - Immunology 2024Quote: ... Cell-bead antibody complex was then washed with cell permeabilization buffer and incubated with pAG-MNase (EpiCypher), followed by addition of 2 mM CaCl2 and incubation for 2 h at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts, Epicypher Cat# 13-0031), Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns ...
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN was performed with anti-HA antibody (HA Tag CUTANA, 13-2010) and a commercial kit (EpiCypher CUTANA pAG-MNase for ChIC/CUT&RUN ...
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bioRxiv - Genetics 2023Quote: ... Bead-bound cells were resuspended in 50 μl secondary antibody buffer (goat anti-rabbit IgG (Epicypher, 13-0047) diluted 1:100 in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 µg of anti-H3K27ac (EpiCypher, 13-0045), 0.6 µg of anti-Stat5 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-ASYM26 (13-0011, Epicypher, 1:1000). Immunofluorescence were performed with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... anti-BRG1 (13-2002, EpiCypher, 1:50 dilution), and non-immune rabbit IgG (2729 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-H3K4me3 (13-0041, EpiCypher, 1:50 dilution), anti-H3K27me3 (9733 ...
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bioRxiv - Biochemistry 2024Quote: ... rabbit anti-H3K9me1 (1:5000, Epicypher 13-0029), rabbit anti-H3K9me2 (1:5000 ...
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bioRxiv - Cancer Biology 2021Quote: CUT&RUN coupled with high-throughput DNA sequencing was performed using antibodies listed in Table S3 and Cutana pA/G-MNase (Epicypher) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... We used immuno-tethered CUT&Tag chromatin profiling [Kaya-Okur et al, 2020] with antibodies to histone H3K27me3 (C36B11, Cell Signalling Technology) and to histone H3K4me2 (13-0027, Epicypher) modifications ...
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bioRxiv - Plant Biology 2024Quote: ... nuclei were resuspended in Antibody150 buffer and from antibody incubation to tagmentation with pAG-Tn5 (EpiCypher Cat No. 15-1017) we followed the CUT&Tag Protocol v1.7 from CUTANA™ ...
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bioRxiv - Genetics 2019Quote: ... and H3(1-43) peptides were obtained from EpiCypher. Binding reactions consisting of protein (13 µg) ...
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bioRxiv - Biochemistry 2022Quote: ... Cell-bead solution was then buffer exchanged into Antibody Buffer and target-specific antibodies added to individual reaction tubes in the following amounts: 0.5 µg of anti-H3K4me3 (EpiCypher, 13-0041), 1 µg of anti-H3K27ac (EpiCypher ...
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bioRxiv - Genetics 2022Quote: ... and H3K4me3 (at 1:2,000 dilution; Epicypher, Cat#13-0041), followed by an anti-rabbit secondary antibody (used at 1:2,000 dilution) ...
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bioRxiv - Genomics 2023Quote: ... then 1:20 CUTANA™ pAG-Tn5 (Epicypher 15-1017), or 1:250 pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
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bioRxiv - Genetics 2022Quote: ... containing 150 ng of DNA for leaf or 350 ng for ear was then diluted to a total volume of 1500 μL in 1X Wash Buffer and used as input for the CUT&Tag-IT Assay Kit with both anti-CENH3 (maize) antibodies (26) and IgG for each sample (Epicypher 13-0042). Libraries were amplified with 15 or 16 cycles of PCR ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies (FOXP1 Cell Signaling Technologies #2005S, FOXP2 Abcam #ab16046, FOXP4 Millipore #ABE74, H3K27me3 Cell Signaling Technologies #9733, IgG Epicypher #13-0042) were added at 1:50 dilution and samples were nutated overnight at 4C.
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bioRxiv - Cancer Biology 2023Quote: ... 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004), and 1 μCi of 3H-SAM (PerkinElmer ...
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bioRxiv - Molecular Biology 2022Quote: ... To localize MLL1 or RNA polymerase II standard CUT&RUN (Skene et al, 2018) was employed using MLL1/KMT2A antibodies (Epicypher #SKU 13-2004) or 8WG16 antibodies directed against the CTD of RPB1 at 1 μg/ml ...
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bioRxiv - Genetics 2022Quote: ... loaded with Illumina NEXTERA adapters (a kind gift from the Henikoff lab, via Madapura lab, diluted 1:250, or Epicypher CUTANA® pAG-Tn5, diluted 1:10) was prepared in Dig-300 Buffer (0.05% Digitonin ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μl E-Coli spike-in DNA (0.5 ng/μl) (EpiCypher) to each tube ...
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bioRxiv - Biochemistry 2021Quote: ... the arrays were incubated with a 1:1000 dilution of anti-GST (EpiCypher, 13-0022) in 1% milk 1x PBST for two hours at 4oC with shaking ...
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bioRxiv - Developmental Biology 2023Quote: ... a 1:5 dilution of K-MetStat Panel spike-in nucleosomes (EpiCypher, cat. # 19-1002) was added to each reaction prior to addition of antibody ...
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bioRxiv - Genetics 2023Quote: ... or CUTANA™ CUT&RUN Library Prep Kit with Primer Set 1 (14-1001, EpiCypher), and library concentrations were quantified with the Qubit 1 X dsDNA HS Assay Kit (Q33231 ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell-bead slurry was washed and incubated with pAG-MNase (1:20 dilution, EpiCypher, 15-1116) for 10 min at RT ...
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bioRxiv - Genomics 2023Quote: ... resuspended in pAG-Tn5 pre-loaded with mosaicend adapters (EpiCypher cat. no. 15-1117, 1:20 dilution) in 300-Wash buffer (Wash buffer except containing 300 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were washed with digitonin buffer resuspended in buffer containing pAG-MNase (CUTANA EpiCypher, 1:20 dilution) and digitonin ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% digitonin) and resuspended in 50 μl cold Dig-Wash buffer with 1 μl pAG-MNase (EpiCypher, 15-1016) per reaction ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were washed four times in Dig-Wash and then incubated with a 1:20 dilution of pAG-Tn5 adapter complex (EpiCypher) in Dig-300 buffer (1x Protease inhibitor cocktail ...
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bioRxiv - Immunology 2024Quote: ... Cells were then permeabilized and incubated overnight on a nutator at 4°C with 1 μg of normal rabbit IgG (EpiCypher), KLF4 (proteintech ...
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bioRxiv - Genomics 2021Quote: ... then the beads were resuspended in pAG-Tn5 pre-loaded with mosaic-end adapters (Epicypher cat. no. 15-1117 1:20) in 300-wash buffer (Wash buffer except containing 300 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... For negative control samples (e.g. rabbit α-mouse IgG) as well as H3K27me3 samples 1 µL of SNAP-CUTANA K-MetStat Panel (Epicypher #19-1002) was added to each sample ...