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Citations for EpiCypher :
1 - 50 of 105 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... nuclei were isolated from 100,000 primary human macrophages and bound to activated magnetic concanavalin A beads (Epicypher). The bound nuclei were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were incubated on activated Concanavalin A conjugated paramagnetic beads (EpiCypher) at room temperature for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: A total of 500,000 cells per reaction were harvested and captured using 10 µL of pre-activated ConA beads (EpiCypher). Beads with attached cells were incubated at room temperature for 10 min to ensure complete adsorption ...
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bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...
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bioRxiv - Genomics 2021Quote: ... and bound to activated Concanavalin A coated magnetic beads (Epicypher 21-1401). Cells were then permeabilized with Wash buffer (20mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... Five-hundred thousand nuclei were counted and incubated with activated Concanavalin A coated beads (EpiCypher 21-1411) and subsequently mixed with antibody (Table S14 ...
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bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
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bioRxiv - Cancer Biology 2024Quote: ... About 900’000 cells were processed using the CUTANA kit (Epicypher, #14-1048). Briefly ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Molecular Biology 2023Quote: ... Illumina sequencing libraries were prepared from ∼5 ng of purified DNA using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001 & 14-1002) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... CX3CR1Pos cells were subjected to CUT&RUN using the CUTANA™ ChIC/CUT&RUN kit (Epicypher) using an anti-FLAG antibody (Sigma M2 #1804 ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Biochemistry 2022Quote: Chromatin profiling for WT and ACK KO K562 cells was performed using CUTANA™ ChIC/CUT&RUN Kit (EpiCypher) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... All subsequent steps through to library preparation and purification f ollowed t he standard CUT&Tag-direct protocol (19) using pAG-Tn5 (Epicypher cat. no. 15-1117), except that 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated cells were counted in haemocytometer and approximately 250,000 cells/sample were used for further processing using CUTANA ChIC/ CUT & RUN kit (Epicypher, 14-1048) according to the manufacture’s protocol ...
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bioRxiv - Genomics 2022Quote: CUT&RUN was performed on in-vitro polarized Th1 cells using CUTANA™ ChIC/CUT&RUN Kit (EpiCypher, Cat#14-1048), using manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
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bioRxiv - Systems Biology 2023Quote: ... Profiling of chromatin modifications at the CD43 endogenous gene in K562 cells was performed using the EpiCypher CUTANA ChIC/CUT&RUN kit (EpiCypher, 14-1048), according to manufacturer instructions (Kit v3) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Biochemistry 2022Quote: ... and 5’ biotinylated H3K4me3 187 bp mononucleosomes (16-2316) were purchased from Epicypher, Inc. ...
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bioRxiv - Developmental Biology 2023Quote: ... a 1:5 dilution of K-MetStat Panel spike-in nucleosomes (EpiCypher, cat. # 19-1002) was added to each reaction prior to addition of antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... the 14-1048 CUT&RUN kit by EpiCypher was utilized according to EpiCypher’s protocol ...
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bioRxiv - Biochemistry 2023Quote: All PTM-defined nucleosomes (Supplementary file 5; homotypic unless stated otherwise) were from the dNucTM or versaNuc® portfolios (EpiCypher). PTMs were confirmed by mass-spectrometry and immunoblotting (if an antibody was available ...
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bioRxiv - Cancer Biology 2022Quote: CUTnRUN was performed using CUTANA CUT&RUN kit (Epicypher) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... coli Spike-In DNA (provided with the EpiCypher kit) into the STOP buffer for quenching pAG-MNase activity ...
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bioRxiv - Genetics 2023Quote: ... using CUTANA ChIC/CUT&RUN Kit (14-1048, EpiCypher) and H3K9me3 antibody (C15410193 ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were processed using CUTANA™ CUT&RUN kit (EpiCypher) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and CUTANA CUT&RUN Library Prep Kit (EpiCypher, 14-1002). The protocol was carried out according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: Cut&Run-seq was performed using CUTANA v3 kit (Epicypher) according to the manufacturer’s protocol and with mild crosslinking (1 min incubation with 1% formaldehyde at room temperature) ...
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bioRxiv - Immunology 2024Quote: ... Cell-bead antibody complex was then washed with cell permeabilization buffer and incubated with pAG-MNase (EpiCypher), followed by addition of 2 mM CaCl2 and incubation for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with pAG-MNase (Epicypher) in dig wash buffer then in reaction buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA Fragments were then purified using CUTANA DNA Purification Kit (EpiCypher).
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN was performed using CUTANA™ Kit (EpiCypher #14-1048) with minor modifications according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using a CUTANA ChIC/CUT&RUN Kit (EpiCypher) per manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: We performed CUT&RUN assay with CUTANA kit (EpiCypher, 14-1048) according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated with pAG-MNase (EpiCypher) for 10 minutes at room temperature and washed with Digitonin Buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... the DNA was purified using CUTANA DNA Purification Kit (EpiCypher, 14-0050) according to the manufacturer instructions ...
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bioRxiv - Genetics 2023Quote: The CUT & RUN protocol (CUTANA ChIC/CUT & RUN Kit, EpiCypher, #14-1048) was performed according to manufacturer protocols with optimizations for A253 cells ...
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bioRxiv - Molecular Biology 2023Quote: Cut&Run was performed using the CUTANA™ CUT&RUN Kit (Epicypher) on non-fixed nuclei according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... CUT&RUN for CEBPβ was performed using CUTANA CUT&RUN Kit (EpiCypher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using the CUTANA™ ChIC/CUT&RUN Kit (Epicypher®, 14-1048). IMR90 cells were washed once with PBS and scraped ...
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bioRxiv - Cell Biology 2024Quote: ... CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher) using 260,000 to 500,000 cells/sample and the following antibodies ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies (FOXP1 Cell Signaling Technologies #2005S, FOXP2 Abcam #ab16046, FOXP4 Millipore #ABE74, H3K27me3 Cell Signaling Technologies #9733, IgG Epicypher #13-0042) were added at 1:50 dilution and samples were nutated overnight at 4C.
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bioRxiv - Genomics 2023Quote: ... DNA libraries were constructed using the CUTANA CUT&RUN Library Prep Kit (EpiCypher) per manufacturer’s instructions ...