Labshake search
Citations for EpiCypher :
1 - 50 of 73 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
-
bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
-
bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
-
bioRxiv - Immunology 2022Quote: ... then resuspended and incubated at 4°C for 1 hour in CUT&RUN antibody buffer and 2.5 μL pAG-MNase (EpiCypher). ConA-bound-nuclei were then washed twice with CUT&RUN triton wash buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then permeabilized and incubated overnight on a nutator at 4°C with 1 μg of normal rabbit IgG (EpiCypher), KLF4 (proteintech ...
-
bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
-
bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
-
bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
-
bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
-
bioRxiv - Biophysics 2020Quote: ... Histone proteins were purchased from EpiCypher. While the H3 histones were available as part of recombinant human histone octamers ...
-
bioRxiv - Biochemistry 2021Quote: ... and incubated overnight (4°C with gentle rocking) with 1 µg of antibody (For all 40 PCRP antibodies as above; RbIgG (EpiCypher 13-0042, lot 20036001-52); MsIgG (Invitrogen 10400C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein A-micrococcal nuclease (pA-MN; EpiCypher donation) was added to a concentration of 1:400 to nuclei ...
-
bioRxiv - Developmental Biology 2023Quote: ... we used protein A/G-MNase from Epicypher. For 24h pupal samples with 40 and 75 wings ...
-
bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
-
bioRxiv - Cancer Biology 2021Quote: ... After overnight incubation at 4°C with the primary FOXA1 antibody (13-2001, Epicypher), cell-bead slurry was washed twice more after which pA-MNase digestion was activated by placing samples on an ice-cold block and incubated with digitonin wash buffer containing 2 mM CaCl2 ...
-
bioRxiv - Immunology 2021Quote: ... nuclei were isolated from 100,000 primary human macrophages and bound to activated magnetic concanavalin A beads (Epicypher). The bound nuclei were incubated with primary antibody overnight at 4°C ...
-
bioRxiv - Genomics 2023Quote: ... The following commercial loaded protein A-Tn5 transposase fusion protein (pA-Tn5) were used at recommended dilutions by the manufacturer: CUTANA™ pAG-Tn5 (Epicypher 15-1017; Lot 20142001-C1), or pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins (0.4 μM) were mixed with nucleosome (50N66, 0.03 μM - Epicypher) in 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
-
bioRxiv - Molecular Biology 2021Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). Nucleosome reconstitutions were performed by salt gradient dialysis64 ...
-
bioRxiv - Biochemistry 2022Quote: ... coli (ISWI, ModT antigen and LCMS reference proteins) or obtained from EpiCypher Inc ...
-
bioRxiv - Biophysics 2024Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). We followed the previously published protocol to prepare nucleosome reconstitutions [80] ...
-
bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...
-
bioRxiv - Systems Biology 2023Quote: ... an input of ∼400,000 cells per antibody condition and an overnight antibody incubation at 4°C with H3K4me3 (0.5 μg/ml, EpiCypher), H3K9me3 (0.9 μg/ml ...
-
bioRxiv - Developmental Biology 2023Quote: ... Dissected tissues were dissociated in 100µg/mL Liberase at 37°C for 10minutes and processed using EpiCypher’s CUTANA kit reagents (EpiCypher 15-1016) and protocol with the following modifications ...
-
bioRxiv - Genomics 2020Quote: ... The pAG-Tn5 fusion protein used in many of these experiments was a gift from Epicypher, Inc ...
-
bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
-
bioRxiv - Immunology 2021Quote: ... This was followed by washing and incubation with the protein A/G conjugated Tn5 (CUTANA pAG-Tn5, Epicypher) enzyme for one hour at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cell-bead slurry was washed twice with Digitonin Buffer and incubated with 1x Protein-A/G-MNase (pAG-MNase; Epicypher) in Digitonin Buffer for 10 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... Beads were washed in Wash buffer 300 (Wash buffer containing 300mM NaCl) two times and resuspended in Wash buffer 300 containing Protein A/G-TN5 (EpiCypher) and incubated for an hour at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
-
bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
-
bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041, 1:50], or rabbit IgG negative control antibody [Epicypher, 13-0042, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
-
bioRxiv - Genomics 2021Quote: ... and incubated for 1 hour at room temperature for the guinea pig anti-rabbit secondary antibody (Antibodies Online cat. no. ABIN101961 1:100) and for 1 hour at room temperature for pAG-Tn5 (Epicypher cat. no. 15-1117, 1:20) tethering ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:100 H3K4me3 Antibody (Epicypher, #13-0041), 1:50 H3K27me3 antibody (Cell Signaling ...
-
bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts, Epicypher Cat# 13-0031), Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 µg of anti-H3K27ac (EpiCypher, 13-0045), 0.6 µg of anti-Stat5 (Santa Cruz Biotechnology ...
-
bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-ASYM26 (13-0011, Epicypher, 1:1000). Immunofluorescence were performed with the following antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-BRG1 (13-2002, EpiCypher, 1:50 dilution), and non-immune rabbit IgG (2729 ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-H3K4me3 (13-0041, EpiCypher, 1:50 dilution), anti-H3K27me3 (9733 ...
-
bioRxiv - Biochemistry 2024Quote: ... rabbit anti-H3K9me1 (1:5000, Epicypher 13-0029), rabbit anti-H3K9me2 (1:5000 ...
-
bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5μg of rabbit glucocorticoid receptor antibody [abcam, ab225886, 1:20] or rabbit IgG negative control antibody [Epicypher, 13-0041k, 1:50]). The remaining steps were performed according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... and H3(1-43) peptides were obtained from EpiCypher. Binding reactions consisting of protein (13 µg) ...
-
bioRxiv - Genetics 2022Quote: ... and H3K4me3 (at 1:2,000 dilution; Epicypher, Cat#13-0041), followed by an anti-rabbit secondary antibody (used at 1:2,000 dilution) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated with H3 antibody (1:50,000; EpiCypher 13-0001) or H3K27me3 antibody (1:2,000 ...
-
bioRxiv - Genomics 2023Quote: ... then 1:20 CUTANA™ pAG-Tn5 (Epicypher 15-1017), or 1:250 pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:100 Rabbit IgG Negative Control Antibody (Epicypher, #13-0042) and incubated overnight at 4°C ...