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Citations for EpiCypher :
1 - 50 of 130 citations for Human Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
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bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
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bioRxiv - Cell Biology 2023Quote: ... Protein A/G-fused Tn5 transposase protein (CUTANA pA/G-Tn5, Epicypher) was added and the samples were incubated for 1 hour at room temperature followed by washing with a buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Biophysics 2020Quote: ... Histone proteins were purchased from EpiCypher. While the H3 histones were available as part of recombinant human histone octamers ...
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bioRxiv - Genetics 2023Quote: ... or CUTANA™ CUT&RUN Library Prep Kit with Primer Set 1 (14-1001, EpiCypher), and library concentrations were quantified with the Qubit 1 X dsDNA HS Assay Kit (Q33231 ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein A-micrococcal nuclease (pA-MN; EpiCypher donation) was added to a concentration of 1:400 to nuclei ...
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bioRxiv - Developmental Biology 2023Quote: ... we used protein A/G-MNase from Epicypher. For 24h pupal samples with 40 and 75 wings ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Immunology 2021Quote: ... nuclei were isolated from 100,000 primary human macrophages and bound to activated magnetic concanavalin A beads (Epicypher). The bound nuclei were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Genomics 2023Quote: ... The following commercial loaded protein A-Tn5 transposase fusion protein (pA-Tn5) were used at recommended dilutions by the manufacturer: CUTANA™ pAG-Tn5 (Epicypher 15-1017; Lot 20142001-C1), or pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins (0.4 μM) were mixed with nucleosome (50N66, 0.03 μM - Epicypher) in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
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bioRxiv - Molecular Biology 2021Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). Nucleosome reconstitutions were performed by salt gradient dialysis64 ...
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bioRxiv - Biochemistry 2022Quote: ... coli (ISWI, ModT antigen and LCMS reference proteins) or obtained from EpiCypher Inc ...
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bioRxiv - Biophysics 2024Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). We followed the previously published protocol to prepare nucleosome reconstitutions [80] ...
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bioRxiv - Genomics 2020Quote: ... The pAG-Tn5 fusion protein used in many of these experiments was a gift from Epicypher, Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... This was followed by washing and incubation with the protein A/G conjugated Tn5 (CUTANA pAG-Tn5, Epicypher) enzyme for one hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... the 14-1048 CUT&RUN kit by EpiCypher was utilized according to EpiCypher’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: CUTnRUN was performed using CUTANA CUT&RUN kit (Epicypher) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... coli Spike-In DNA (provided with the EpiCypher kit) into the STOP buffer for quenching pAG-MNase activity ...
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bioRxiv - Genetics 2023Quote: ... using CUTANA ChIC/CUT&RUN Kit (14-1048, EpiCypher) and H3K9me3 antibody (C15410193 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cell-bead slurry was washed twice with Digitonin Buffer and incubated with 1x Protein-A/G-MNase (pAG-MNase; Epicypher) in Digitonin Buffer for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed in Wash buffer 300 (Wash buffer containing 300mM NaCl) two times and resuspended in Wash buffer 300 containing Protein A/G-TN5 (EpiCypher) and incubated for an hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were processed using CUTANA™ CUT&RUN kit (EpiCypher) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and CUTANA CUT&RUN Library Prep Kit (EpiCypher, 14-1002). The protocol was carried out according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: Cut&Run-seq was performed using CUTANA v3 kit (Epicypher) according to the manufacturer’s protocol and with mild crosslinking (1 min incubation with 1% formaldehyde at room temperature) ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041, 1:50], or rabbit IgG negative control antibody [Epicypher, 13-0042, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Genomics 2021Quote: ... and incubated for 1 hour at room temperature for the guinea pig anti-rabbit secondary antibody (Antibodies Online cat. no. ABIN101961 1:100) and for 1 hour at room temperature for pAG-Tn5 (Epicypher cat. no. 15-1117, 1:20) tethering ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA Fragments were then purified using CUTANA DNA Purification Kit (EpiCypher).
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN was performed using CUTANA™ Kit (EpiCypher #14-1048) with minor modifications according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using a CUTANA ChIC/CUT&RUN Kit (EpiCypher) per manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: We performed CUT&RUN assay with CUTANA kit (EpiCypher, 14-1048) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... the DNA was purified using CUTANA DNA Purification Kit (EpiCypher, 14-0050) according to the manufacturer instructions ...
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bioRxiv - Genetics 2023Quote: The CUT & RUN protocol (CUTANA ChIC/CUT & RUN Kit, EpiCypher, #14-1048) was performed according to manufacturer protocols with optimizations for A253 cells ...
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bioRxiv - Molecular Biology 2023Quote: Cut&Run was performed using the CUTANA™ CUT&RUN Kit (Epicypher) on non-fixed nuclei according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... CUT&RUN for CEBPβ was performed using CUTANA CUT&RUN Kit (EpiCypher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... About 900’000 cells were processed using the CUTANA kit (Epicypher, #14-1048). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... using the CUTANA™ ChIC/CUT&RUN Kit (Epicypher®, 14-1048). IMR90 cells were washed once with PBS and scraped ...
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bioRxiv - Cell Biology 2024Quote: ... CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher) using 260,000 to 500,000 cells/sample and the following antibodies ...