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Citations for EpiCypher :
1 - 50 of 96 citations for Anti Histone H2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Experiments done with excess ubiquitinated nucleosome used purified recombinant mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-K120Ub) (EpiCypher, 16-0370) at 400 nM and 25 nM SAGA ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Genetics 2020Quote: ... We used immuno-tethered CUT&Tag chromatin profiling [Kaya-Okur et al, 2020] with antibodies to histone H3K27me3 (C36B11, Cell Signalling Technology) and to histone H3K4me2 (13-0027, Epicypher) modifications ...
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bioRxiv - Cancer Biology 2023Quote: All histone markers antibodies for CUT&RUN were either certified by EpiCypher (H3K4me1, H3K4me3, H3K27me3) or validated by SNAP-ChIP qPCR (H3K27Ac) ...
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bioRxiv - Biophysics 2020Quote: ... Histone proteins were purchased from EpiCypher. While the H3 histones were available as part of recombinant human histone octamers ...
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bioRxiv - Biophysics 2024Quote: ... The CENP-A/H4 tetramer and H2A/H2B dimer are ordered from EpiCypher (Durham, NC)
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bioRxiv - Plant Biology 2024Quote: ... Histone peptides utilized in this study were synthesized by EpiCypher and included H3 (1–32 aa) ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Biochemistry 2019Quote: ... and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311, EpiCypher Research Triangle Park, NC) nucleosomes were performed as previously described (47 ...
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bioRxiv - Biochemistry 2019Quote: ... and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311, EpiCypher Research Triangle Park, NC) on a 3kb plasmid containing a single 601 sequence (pGEM3Z-601 from Addgene #26656 ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human CENP-A/H4 tetramers and H2A/H2B dimers were purchased from EpiCypher (Research Triangle Park, NC), while for H3 nucleosomes ...
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bioRxiv - Biophysics 2023Quote: ... and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311, EpiCypher Research Triangle Park, NC) nucleosomes were performed as previously described (Dimitriadis et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). Nucleosome reconstitutions were performed by salt gradient dialysis64 ...
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bioRxiv - Biophysics 2024Quote: ... Unmodified and modified histone proteins were purchased from EpiCypher (Durham, North Carolina). We followed the previously published protocol to prepare nucleosome reconstitutions [80] ...
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bioRxiv - Biochemistry 2020Quote: Biotinylated histone peptides for the dCypher assays were from EpiCypher (Resources Table A). Histone peptides for other studies were synthesized by the Peptide Core Facility at the University of Colorado Denver or by GenScript with a free N-terminus and an amidated C-terminus ...
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bioRxiv - Genomics 2023Quote: 10-20ng of W601 sequence (free and histone bound, cat. 16-0009, Epicypher) was combined with antibody in 200μL of PBS ...
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bioRxiv - Genomics 2022Quote: ... 0.5ug secondary antibody (CUTANA anti-rabbit secondary antibody; 13-0047; Epicypher) was incubated for 0.5hr at RT ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Biochemistry 2019Quote: In vitro reconstitution of CENP-A (CENP-A/H4 cat#16-010 and H2A/H2B cat#15-0311, EpiCypher, Research Triangle Park, NC) and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311 ...
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bioRxiv - Biophysics 2023Quote: In vitro reconstitution of CENP-A (CENP-A/H4 cat#16-010 and H2A/H2B cat#15-0311, EpiCypher, Research Triangle Park, NC) and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311 ...
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bioRxiv - Biochemistry 2019Quote: ... and CENP-A mononucleosome (CENP-A/H4 cat#16-010, H2A/H2B cat#15-0311, 187bp of 601-sequence cat#18-2003, EpiCypher, Research Triangle Park, NC) samples were diluted 1:5 in 2 mM NaCl with 4 mM MgCl (pH7.5 ...
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bioRxiv - Biochemistry 2019Quote: In vitro reconstitution of CENP-A nucleosome arrays (CENP-A/H4 cat#16-010 and H2A/H2B cat#15-0311, EpiCypher, Research Triangle Park, NC) and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Digitonin and 0.5 μg of anti-rabbit or anti-mouse secondary antibodies (Epicypher). After incubation for 30 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Another modification was to spike-in the panel of synthetic nucleosomes carrying major histone methylations (EpiCypher, SKU: 19-1001) (34) ...
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bioRxiv - Developmental Biology 2022Quote: ... Then 0.5 μg anti-rabbit IgG secondary antibody (EpiCypher, 13-0047) was added to each sample and incubated on a nutator for 30 min at room temperature ...
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bioRxiv - Biophysics 2023Quote: H3K27ac nucleosomes were assembled using the LIN28B DNA (unlabeled or Cy5-labeled) and histone octamer with H3K27ac modification (custom purchased from Epicypher). For H3K27-trimethyl nucleosomes ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of Rabbit anti-H3K4me3 ChIP grade antibody (Epicypher, 13-0041), which has been SNAP-ChIP validated for H3K4me3 specificity 62 ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rabbit antibody for CUTANA™ CUT&Tag Workflows (13-0047, EpiCypher).
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bioRxiv - Cell Biology 2023Quote: ... the following primary antibodies were used: anti-H3K9ac (rabbit, Epicypher 13-0033, 1:500), anti-γH2Av (mouse ...
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bioRxiv - Cancer Biology 2024Quote: ... This incubation/wash cycle was repeated for the guinea pig anti-rabbit secondary antibody (Antibodies Online cat. no. ABIN101961) and for pAG-Tn5 preloaded with mosaic end adapters (Epicypher cat. no. 15-1117 1:20), followed by a Triton-Wash rinse and transfer of the slide to 10 mM TAPS pH 8.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized using 0.01% digitonin and incubated with 0.5μg antibody (anti-Sox9 (CST, #82630) and IgG (# 13-0042; Epicypher) in 50μL antibody buffer (20mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... & protease inhibitors) followed by the addition of 0.5 µg CUTANA anti-rabbit secondary antibody (#13-1047, EpiCypher). Samples were then incubated on nutator at room temperature for 1 hour to facilitate binding ...
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN was performed with anti-HA antibody (HA Tag CUTANA, 13-2010) and a commercial kit (EpiCypher CUTANA pAG-MNase for ChIC/CUT&RUN ...
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bioRxiv - Genetics 2023Quote: ... Bead-bound cells were resuspended in 50 μl secondary antibody buffer (goat anti-rabbit IgG (Epicypher, 13-0047) diluted 1:100 in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Cell-bead solution was then buffer exchanged into Antibody Buffer and target-specific antibodies added to individual reaction tubes in the following amounts: 0.5 µg of anti-H3K4me3 (EpiCypher, 13-0041), 1 µg of anti-H3K27ac (EpiCypher ...
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bioRxiv - Genetics 2022Quote: ... containing 150 ng of DNA for leaf or 350 ng for ear was then diluted to a total volume of 1500 μL in 1X Wash Buffer and used as input for the CUT&Tag-IT Assay Kit with both anti-CENH3 (maize) antibodies (26) and IgG for each sample (Epicypher 13-0042). Libraries were amplified with 15 or 16 cycles of PCR ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041 ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045 ...
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bioRxiv - Bioengineering 2023Quote: ... H3K27ac antibody (Epicypher, #13-0045) or input DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... HA antibody (EpiCypher, 13-2010), PA antibody (Fuji Film ...
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bioRxiv - Cell Biology 2023Quote: ... BRD4 antibody (Epicypher #13-2003) or normal rabbit IgG (Cell Signaling Technology #2729 ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041, 1:50], or rabbit IgG negative control antibody [Epicypher, 13-0042, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to each sample (RELA antibody [Santa Cruz, sc-109, 1:25] or rabbit IgG negative control antibody [EpiCypher, 13-0041, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used were ER (Epicypher, #13-2012), pS118-ER (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... H3K27Ac mouse monoclonal antibody (EpiCypher, # 13-0045), HIRA(34) ...
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bioRxiv - Molecular Biology 2023Quote: ... H3K4Me1 rabbit polyclonal antibody (EpiCypher, # 13-0040), H3K27Ac mouse monoclonal antibody (EpiCypher ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:100 H3K4me3 Antibody (Epicypher, #13-0041), 1:50 H3K27me3 antibody (Cell Signaling ...