Labshake search
Citations for EpiCypher :
1 - 50 of 55 citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... a 1:5 dilution of K-MetStat Panel spike-in nucleosomes (EpiCypher, cat. # 19-1002) was added to each reaction prior to addition of antibody ...
-
bioRxiv - Genetics 2023Quote: ... 2 μl /1×106 cells of the following antibodies were used for immunoprecipitations: H3K4me3 (EpiCypher 13-00041), H3K9me3 (Active Motif 39161) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 µL i7 barcoded primer (EpiCypher), 0.005 ng Escherichia coli spike-in DNA (made in-house) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 µL i5 universal primer (EpiCypher), 2 µL i7 barcoded primer (EpiCypher) ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Liquid nitrogen or OCT embedded frozen kidney tissue from 6 donors was processed according to a modified protocol adapted from Epicypher and available on protocols.io47 ...
-
bioRxiv - Biochemistry 2022Quote: ... and 5’ biotinylated H3K4me3 187 bp mononucleosomes (16-2316) were purchased from Epicypher, Inc. ...
-
bioRxiv - Molecular Biology 2020Quote: ... proteins at 10 nM were incubated for 5 minutes with 20 nM 225 bp nucleosomes (EpiCypher) at 30 °C in 20 mM Tris pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 µl of SNAP-ChIP K-MetStat panel and K-AcylStat panel nucleosomes (EpiCypher) respectively ...
-
bioRxiv - Biochemistry 2023Quote: All PTM-defined nucleosomes (Supplementary file 5; homotypic unless stated otherwise) were from the dNucTM or versaNuc® portfolios (EpiCypher). PTMs were confirmed by mass-spectrometry and immunoblotting (if an antibody was available ...
-
bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041, 1:50], or rabbit IgG negative control antibody [Epicypher, 13-0042, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
-
bioRxiv - Biochemistry 2022Quote: ... a recombinant ATPase over a concentration range (Figure 2-figure supplement 1G-J) was mixed with 10 nM EpiDyne biotinylated nucleosome remodeling substrate (EpiCypher), terminally positioned 6-N-66 (219 bp fragment ...
-
bioRxiv - Genomics 2021Quote: ... and incubated for 1 hour at room temperature for the guinea pig anti-rabbit secondary antibody (Antibodies Online cat. no. ABIN101961 1:100) and for 1 hour at room temperature for pAG-Tn5 (Epicypher cat. no. 15-1117, 1:20) tethering ...
-
bioRxiv - Microbiology 2022Quote: 1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
-
bioRxiv - Molecular Biology 2024Quote: ... beads were washed twice with 200 µl 0.25 % Digitonin wash buffer and resuspended in Digitonin wash buffer containing 2 µl CUTANA™ pAG-MNase (15-1016, EpiCypher, 15-1016) and incubated on ice for 30 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:100 H3K4me3 Antibody (Epicypher, #13-0041), 1:50 H3K27me3 antibody (Cell Signaling ...
-
bioRxiv - Molecular Biology 2023Quote: ... Illumina sequencing libraries were prepared from ∼5 ng of purified DNA using the CUTANA™ CUT&RUN Library Prep Kit (EpiCypher 14-1001 & 14-1002) according to the manufacturer’s recommendations ...
-
bioRxiv - Genomics 2021Quote: ... Rabbit anti-H3K36me3 (1:100 for bulk profiling or 1:10 for single-cell experimemts, Epicypher Cat# 13-0031), Rabbit anti-H3K27me3 (1:100 for bulk profiling or 1:10 for single-cell experimetns ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 µg of anti-H3K27ac (EpiCypher, 13-0045), 0.6 µg of anti-Stat5 (Santa Cruz Biotechnology ...
-
bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-ASYM26 (13-0011, Epicypher, 1:1000). Immunofluorescence were performed with the following antibodies ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-BRG1 (13-2002, EpiCypher, 1:50 dilution), and non-immune rabbit IgG (2729 ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-H3K4me3 (13-0041, EpiCypher, 1:50 dilution), anti-H3K27me3 (9733 ...
-
bioRxiv - Biochemistry 2024Quote: ... rabbit anti-H3K9me1 (1:5000, Epicypher 13-0029), rabbit anti-H3K9me2 (1:5000 ...
-
bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5μg of rabbit glucocorticoid receptor antibody [abcam, ab225886, 1:20] or rabbit IgG negative control antibody [Epicypher, 13-0041k, 1:50]). The remaining steps were performed according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... and H3(1-43) peptides were obtained from EpiCypher. Binding reactions consisting of protein (13 µg) ...
-
bioRxiv - Genetics 2022Quote: ... and H3K4me3 (at 1:2,000 dilution; Epicypher, Cat#13-0041), followed by an anti-rabbit secondary antibody (used at 1:2,000 dilution) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated with H3 antibody (1:50,000; EpiCypher 13-0001) or H3K27me3 antibody (1:2,000 ...
-
bioRxiv - Genomics 2023Quote: ... then 1:20 CUTANA™ pAG-Tn5 (Epicypher 15-1017), or 1:250 pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1:100 Rabbit IgG Negative Control Antibody (Epicypher, #13-0042) and incubated overnight at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... Antibodies were added to each sample (RELA antibody [Santa Cruz, sc-109, 1:25] or rabbit IgG negative control antibody [EpiCypher, 13-0041, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 µM recombinant nucleosomes wrapped with 187bp DNA (EpiCypher 16-2004), and 1 μCi of 3H-SAM (PerkinElmer ...
-
bioRxiv - Genetics 2022Quote: ... loaded with Illumina NEXTERA adapters (a kind gift from the Henikoff lab, via Madapura lab, diluted 1:250, or Epicypher CUTANA® pAG-Tn5, diluted 1:10) was prepared in Dig-300 Buffer (0.05% Digitonin ...
-
bioRxiv - Cell Biology 2023Quote: ... and 1 μl E-Coli spike-in DNA (0.5 ng/μl) (EpiCypher) to each tube ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 μg of Rabbit anti-H3K4me3 ChIP grade antibody (Epicypher, 13-0041), which has been SNAP-ChIP validated for H3K4me3 specificity 62 ...
-
bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... the following primary antibodies were used: anti-H3K9ac (rabbit, Epicypher 13-0033, 1:500), anti-γH2Av (mouse ...
-
bioRxiv - Biochemistry 2021Quote: ... the arrays were incubated with a 1:1000 dilution of anti-GST (EpiCypher, 13-0022) in 1% milk 1x PBST for two hours at 4oC with shaking ...
-
bioRxiv - Genetics 2023Quote: ... or CUTANA™ CUT&RUN Library Prep Kit with Primer Set 1 (14-1001, EpiCypher), and library concentrations were quantified with the Qubit 1 X dsDNA HS Assay Kit (Q33231 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041 ...
-
bioRxiv - Cancer Biology 2023Quote: ... the cell-bead slurry was washed and incubated with pAG-MNase (1:20 dilution, EpiCypher, 15-1116) for 10 min at RT ...
-
bioRxiv - Genomics 2023Quote: ... resuspended in pAG-Tn5 pre-loaded with mosaicend adapters (EpiCypher cat. no. 15-1117, 1:20 dilution) in 300-Wash buffer (Wash buffer except containing 300 mM NaCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... the beads were washed with digitonin buffer resuspended in buffer containing pAG-MNase (CUTANA EpiCypher, 1:20 dilution) and digitonin ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.5 µg of primary and secondary antibody were used with 1 µL of pA-Tn5 (Epicypher, 15-1017). Antibodies used ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
-
bioRxiv - Immunology 2022Quote: ... then resuspended and incubated at 4°C for 1 hour in CUT&RUN antibody buffer and 2.5 μL pAG-MNase (EpiCypher). ConA-bound-nuclei were then washed twice with CUT&RUN triton wash buffer ...
-
bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5□μg of H3K27ac antibody [Epicypher, 13-0045, 1:50], H3K4me3 antibody [Epicypher, 13-0041 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.01% digitonin) and resuspended in 50 μl cold Dig-Wash buffer with 1 μl pAG-MNase (EpiCypher, 15-1016) per reaction ...
-
bioRxiv - Immunology 2023Quote: ... Nuclei were washed four times in Dig-Wash and then incubated with a 1:20 dilution of pAG-Tn5 adapter complex (EpiCypher) in Dig-300 buffer (1x Protease inhibitor cocktail ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then permeabilized and incubated overnight on a nutator at 4°C with 1 μg of normal rabbit IgG (EpiCypher), KLF4 (proteintech ...
-
bioRxiv - Genomics 2021Quote: ... then the beads were resuspended in pAG-Tn5 pre-loaded with mosaic-end adapters (Epicypher cat. no. 15-1117 1:20) in 300-wash buffer (Wash buffer except containing 300 mM NaCl ...