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Citations for EpiCypher :
1 - 50 of 66 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... pAG-MNase (EpiCypher #15-1016) was added / activated and CUT&RUN enriched DNA purified using the Monarch DNA Cleanup kit (NEB #T1030S) ...
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bioRxiv - Genetics 2022Quote: ... pAG-MNase (EpiCypher 15-1016) was added to nuclei at a concentration of 1:20 and incubated at RT for 10min ...
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bioRxiv - Cancer Biology 2024Quote: ... pAG-MNase (EpiCypher #15-1016) was added / activated (2 hours @ 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... CUTANA pAG-MNase (EpiCypher, #15-1116) fusion protein was added and incubated at 0 °C for 60-90 mins to digest targeted regions of the genome ...
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bioRxiv - Molecular Biology 2023Quote: ... 2ul pA-Tn5 (EpiCypher 15-1017) was added to each sample and tagmentation was performed ...
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bioRxiv - Neuroscience 2023Quote: ... incubated with pA-MN (EpiCypher 15-1016), and washed in wash buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with pAG-MNase (Epicypher, #15-1116) for 10 min at RT ...
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bioRxiv - Genomics 2021Quote: ... The Tn5 was purchased from Epicypher (15-1017) and used at 1:20 ...
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bioRxiv - Genomics 2021Quote: ... we used CUTANA pAG-Tn5 (Epicypher #15-1017) to tagment the genomic regions surrounding each bound antibody complex ...
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bioRxiv - Cell Biology 2023Quote: ... 15 μl Concanavalin A-coated magnetic beads (EpiCypher) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... beads were washed twice with 200 µl 0.25 % Digitonin wash buffer and resuspended in Digitonin wash buffer containing 2 µl CUTANA™ pAG-MNase (15-1016, EpiCypher, 15-1016) and incubated on ice for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Then 2.5 μl CUTANA pAG-Tn5 (EpiCypher, #15-1017) was added and incubated on a nutator for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... pA-Tn5 preloaded with Nextera adapters (Epicypher #15-1117) was diluted 1:20 in dig-300 buffer (20mM HEPES pH7.5 ...
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bioRxiv - Genetics 2023Quote: ... and 0.05% digitonin) containing pAG-Tn5 (Epicypher, 15-1017), and placed on a nutator at room temperature for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... 15 uL of recombinant nucleosomes (SNAP-ChIP, EpiCypher, 19-1001) were fixed with fresh 1% glutaraldehyde ...
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bioRxiv - Genomics 2022Quote: ... and pAG-Tn5 for CUT&Tag (EpiCypher, SKU: 15-1017), with the following exceptions ...
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bioRxiv - Genomics 2023Quote: ... then 1:20 CUTANA™ pAG-Tn5 (Epicypher 15-1017), or 1:250 pA-Tn5 Transposase - loaded (Diagenode C01070001 ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were incubated with pAG-MNase (EpiCypher, #15-1016) for 10 min at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... All human nucleosomes were purchased from Epicypher with the 5’ biotinylated 187 bp DNA sequence containing the 601 positioning sequence ...
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bioRxiv - Immunology 2021Quote: ... Following incubation and digestion CUTANA™ pAG-MNase from EpiCypher (SKU: 15-1116) the digested DNA fragments were release and purified by QIAquick PCR purification kit (#2810) ...
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bioRxiv - Neuroscience 2022Quote: ... Reaction was washed two additional times before pAG-MNAse (15-1016-EPC Epicypher) was added 1:20 in washing buffer and incubated for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant human octamers containing H3 were purchased from EpiCypher. Nucleosome assembly was achieved using the continuous dilution method ...
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bioRxiv - Cell Biology 2023Quote: We used recombinant biotinylated human mononucleosomes (Epicypher 16-0006), H3K4me3 mononucleosomes (Epicypher 16-0316) ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed twice more before incubation with recombinant p-AG MNase (Epicypher 15-1016) at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatant was removed and beads were resuspended in 100μl 1x pAG-MNase (Epicypher #15-1016); 20x stock pAG-MNase ...
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant human histone octamers were purchased from EpiCypher (Durham, North Carolina). Samples were prepared via salt gradient dialysis following the protocol described previously [41] ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell-bead slurry was washed and incubated with pAG-MNase (1:20 dilution, EpiCypher, 15-1116) for 10 min at RT ...
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bioRxiv - Genomics 2023Quote: ... resuspended in pAG-Tn5 pre-loaded with mosaicend adapters (EpiCypher cat. no. 15-1117, 1:20 dilution) in 300-Wash buffer (Wash buffer except containing 300 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5 mM Sper-midine) then incubated in 47.5 μL Wash350 + 2.5 μL pAG-Tn5 (Epicypher 15-1017) for 1 h ...
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bioRxiv - Biochemistry 2019Quote: ... and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311, EpiCypher Research Triangle Park, NC) nucleosomes were performed as previously described (47 ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.5 µg of primary and secondary antibody were used with 1 µL of pA-Tn5 (Epicypher, 15-1017). Antibodies used ...
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bioRxiv - Biochemistry 2019Quote: ... and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311, EpiCypher Research Triangle Park, NC) on a 3kb plasmid containing a single 601 sequence (pGEM3Z-601 from Addgene #26656 ...
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bioRxiv - Neuroscience 2022Quote: CUT&Tag library construction was performed using CUTANA™ High Fidelity 2X PCR Master Mix (15-1018, EpiCypher) following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2023Quote: ... and H3 (H3/H4 cat#16-0008 and H2A/H2B cat#15-0311, EpiCypher Research Triangle Park, NC) nucleosomes were performed as previously described (Dimitriadis et al. ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei-bound beads were washed with antibody buffer and incubated with antibody buffer containing pAG-MNase (15-1116, EpiCypher) at 4°C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... EDTA-free Protease Inhibitor Cocktail in nuclease-free water) containing 2.5 µl pA-Tn5 adapter complex (EpiCypher 15-1017) and placed on a nutator at RT for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% digitonin) and resuspended in 50 μl cold Dig-Wash buffer with 1 μl pAG-MNase (EpiCypher, 15-1016) per reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were then washed three times with digitonin buffer (Table S13) and incubated with PAG-MNase (EpiCypher 15-1116) for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissected tissues were dissociated in 100µg/mL Liberase at 37°C for 10minutes and processed using EpiCypher’s CUTANA kit reagents (EpiCypher 15-1016) and protocol with the following modifications ...
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bioRxiv - Plant Biology 2024Quote: ... nuclei were resuspended in Antibody150 buffer and from antibody incubation to tagmentation with pAG-Tn5 (EpiCypher Cat No. 15-1017) we followed the CUT&Tag Protocol v1.7 from CUTANA™ ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
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bioRxiv - Genomics 2021Quote: ... then the beads were resuspended in pAG-Tn5 pre-loaded with mosaic-end adapters (Epicypher cat. no. 15-1117 1:20) in 300-wash buffer (Wash buffer except containing 300 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The pA-Tn5 adapter complex containing 2.5 µl of 20x CUTANA™ pAG-Tn5 pre-loaded adapter complex (EpiCypher, #15-1017) was prepared in 50 µl of Dig-300 Buffer (20 mM HEPES pH 7.5 ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... (a step-by-step protocol https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3) using CUTANA™ pAG-Tn5 (Epicypher, 15-1017). Quantitative spike-in CUT&Tag was performed by adding Drosophila S2 cells at a 2:1 ratio to mouse spermatogonial cells (20,000 S2 cells to 10,000 mouse cells ...
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bioRxiv - Cancer Biology 2024Quote: CUT&RUN coupled with high-throughput DNA sequencing was performed on isolated nuclei using Cutana pA/G-MNase (Epicypher, 15-1016) according to the manufacturer’s manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed three times with digitonin wash buffer before resuspending in digitonin wash buffer with pAG-MNase (EpiCypher, 15-1116) at a concentration of 1:20 and nutating at 4°C for 1 hour to allow pAG-MNase and epitope binding ...
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bioRxiv - Developmental Biology 2022Quote: ... Two microliters of uniquely barcoded i5 and i7 primers (10 μM) and 25 μl CUTANA High Fidelity 2x PCR Master Mix (EpiCypher, #15-1018) were added to each sample and mixed ...