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Citations for EpiCypher :
151 - 200 of 209 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: CUT&RUN assay libraries for cultured cells or human kidneys were generated with the CUTANA kit (EpiCypher, 14-1048). For cultured cells ...
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bioRxiv - Microbiology 2023Quote: The CUT&RUN libraries were produced utilizing the CUT&RUN Library Prep Kit (EpiCypher, Inc, Catalog No. 14-1001) User Manual Version 1.0 ...
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bioRxiv - Cancer Biology 2023Quote: Cut&Run assays were performed with CUTANATM ChIC/CUT&RUN kit according to the manufacturer’s protocol (EpiCypher, #14-1048). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Paired-end sequencing libraries were prepared using the CUTANA™ CUT&RUN Library Prep Kit (#14-1001, EpiCypher, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Purified CUT&RUN DNA products were subjected to the CUT&RUN Library Prep Kit (EpiCypher, Catalog No. 14-1002) for library construction ...
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bioRxiv - Biochemistry 2019Quote: H3 (H3 mononucleosome on 187bp of 601 sequence cat#16-2004, EpiCypher, Research Triangle Park, NC) and CENP-A mononucleosome (CENP-A/H4 cat#16-010 ...
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bioRxiv - Genomics 2022Quote: ... Antibodies were added to each sample (0.5μg of rabbit glucocorticoid receptor antibody [abcam, ab225886, 1:20] or rabbit IgG negative control antibody [Epicypher, 13-0041k, 1:50]). The remaining steps were performed according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... containing 150 ng of DNA for leaf or 350 ng for ear was then diluted to a total volume of 1500 μL in 1X Wash Buffer and used as input for the CUT&Tag-IT Assay Kit with both anti-CENH3 (maize) antibodies (26) and IgG for each sample (Epicypher 13-0042). Libraries were amplified with 15 or 16 cycles of PCR ...
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bioRxiv - Bioengineering 2023Quote: ... Next each of 3 100ul aliquots (∼1/3 of each 24 well) of cells were processed for H3K4me3 antibody (Epicypher, #13-0041), H3K27ac antibody (Epicypher ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies (FOXP1 Cell Signaling Technologies #2005S, FOXP2 Abcam #ab16046, FOXP4 Millipore #ABE74, H3K27me3 Cell Signaling Technologies #9733, IgG Epicypher #13-0042) were added at 1:50 dilution and samples were nutated overnight at 4C.
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... They were spun down and washed twice with DPBS before proceeding with the CUTANA CUT&RUN kit (Epicypher 14-0050). The experiment was performed with the included IgG and H3K4Me control antibodies and the BRD2 antibody (abcam 197865 ...
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bioRxiv - Molecular Biology 2023Quote: CUT&RUN was performed on nuclei isolated from CGN cultures using the CUTANA ChIC/CUT&RUN kit (EpiCypher #14-1408) as per manufacturer guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... the permeabilized nuclei were divided equally and incubated in 50 µL antibody buffer with either 0.5µg of control rabbit IgG antibody (Epicypher 14-1048) or anti-c-Myc antibody (ThermoFisher Scientific 13-2500 ...
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bioRxiv - Molecular Biology 2022Quote: ... To localize MLL1 or RNA polymerase II standard CUT&RUN (Skene et al, 2018) was employed using MLL1/KMT2A antibodies (Epicypher #SKU 13-2004) or 8WG16 antibodies directed against the CTD of RPB1 at 1 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated cells were counted in haemocytometer and approximately 250,000 cells/sample were used for further processing using CUTANA ChIC/ CUT & RUN kit (Epicypher, 14-1048) according to the manufacture’s protocol ...
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bioRxiv - Genomics 2022Quote: CUT&RUN was performed on in-vitro polarized Th1 cells using CUTANA™ ChIC/CUT&RUN Kit (EpiCypher, Cat#14-1048), using manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-Ub) were obtained from Epicypher (dNuc, 16-0370) and diluted to 2 µM with reaction buffer ...
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bioRxiv - Genetics 2022Quote: ... DNA was resuspended in 0.1X TE and used for library preparation with the CUTANA™CUT&RUN Library Prep Kit (Epicypher 14-1001), according to the v1 manual ...
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bioRxiv - Cancer Biology 2022Quote: CUT&RUN for SOX9 and IgG control in Adenoma and Normal organoids was done using CUTANA ChIC/CUT&RUN kit (Epicypher #14-1048) following manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Profiling of chromatin modifications at the CD43 endogenous gene in K562 cells was performed using the EpiCypher CUTANA ChIC/CUT&RUN kit (EpiCypher, 14-1048), according to manufacturer instructions (Kit v3) ...
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bioRxiv - Cancer Biology 2024Quote: ... Recovered DNA was quantified using PicoGreen and reactions normalized to 5ng DNA (or entirety of the reaction if <5ng DNA was recovered) before preparing sequencing libraries (CUTANA CUT&RUN Library Prep kit; EpiCypher #14-1001). All autoCUT&RUN steps were optimized / performed on Tecan Freedom EVO robotics platforms with gentle rocking for incubation steps and magnetic capture for media exchange / washing steps ...
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bioRxiv - Immunology 2021Quote: ... Following incubation and digestion CUTANA™ pAG-MNase from EpiCypher (SKU: 15-1116) the digested DNA fragments were release and purified by QIAquick PCR purification kit (#2810) ...
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bioRxiv - Neuroscience 2022Quote: ... Reaction was washed two additional times before pAG-MNAse (15-1016-EPC Epicypher) was added 1:20 in washing buffer and incubated for 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 0.35 µM H3(1-21)-GGK-biotin peptide (Abgent) or 0.35 µM recombinant biotinylated human mononucleosomes (16-0006, Epicypher) and 0.2 mM ATP in KiPIK buffer ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to each sample (RELA antibody [Santa Cruz, sc-109, 1:25] or rabbit IgG negative control antibody [EpiCypher, 13-0041, 1:50]). The remaining steps were performed according to the manufacturer’s instructions for cross-linked samples ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μl of ProteinA/G-MNASE fusion protein (dilution 1:60, 15-1016, EpiCypher) in DIG wash buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Experiments done with excess ubiquitinated nucleosome used purified recombinant mononucleosomes containing histone H2B ubiquitinated at K120 (H2B-K120Ub) (EpiCypher, 16-0370) at 400 nM and 25 nM SAGA ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of GST-tagged reader domain was incubated with 5 μL of 10 nM biotinylated nucleosomes (e.g., EpiCypher #16-9001) for 30 minutes at room temperature in 20 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed twice more before incubation with recombinant p-AG MNase (Epicypher 15-1016) at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatant was removed and beads were resuspended in 100μl 1x pAG-MNase (Epicypher #15-1016); 20x stock pAG-MNase ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell-bead slurry was washed and incubated with pAG-MNase (1:20 dilution, EpiCypher, 15-1116) for 10 min at RT ...
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bioRxiv - Genomics 2023Quote: ... resuspended in pAG-Tn5 pre-loaded with mosaicend adapters (EpiCypher cat. no. 15-1117, 1:20 dilution) in 300-Wash buffer (Wash buffer except containing 300 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.5 mM Sper-midine) then incubated in 47.5 μL Wash350 + 2.5 μL pAG-Tn5 (Epicypher 15-1017) for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.5 µg of primary and secondary antibody were used with 1 µL of pA-Tn5 (Epicypher, 15-1017). Antibodies used ...
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bioRxiv - Neuroscience 2022Quote: CUT&Tag library construction was performed using CUTANA™ High Fidelity 2X PCR Master Mix (15-1018, EpiCypher) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... nuclei-bound beads were washed with antibody buffer and incubated with antibody buffer containing pAG-MNase (15-1116, EpiCypher) at 4°C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... EDTA-free Protease Inhibitor Cocktail in nuclease-free water) containing 2.5 µl pA-Tn5 adapter complex (EpiCypher 15-1017) and placed on a nutator at RT for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were then washed three times with digitonin buffer (Table S13) and incubated with PAG-MNase (EpiCypher 15-1116) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% digitonin) and resuspended in 50 μl cold Dig-Wash buffer with 1 μl pAG-MNase (EpiCypher, 15-1016) per reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissected tissues were dissociated in 100µg/mL Liberase at 37°C for 10minutes and processed using EpiCypher’s CUTANA kit reagents (EpiCypher 15-1016) and protocol with the following modifications ...
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bioRxiv - Plant Biology 2024Quote: ... nuclei were resuspended in Antibody150 buffer and from antibody incubation to tagmentation with pAG-Tn5 (EpiCypher Cat No. 15-1017) we followed the CUT&Tag Protocol v1.7 from CUTANA™ ...
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bioRxiv - Genomics 2022Quote: ... we added 4 ul of SNAP-ChIP K-MetStat Panel (EpiCypher) to every 10 ug of chromatin ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
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bioRxiv - Genomics 2021Quote: ... then the beads were resuspended in pAG-Tn5 pre-loaded with mosaic-end adapters (Epicypher cat. no. 15-1117 1:20) in 300-wash buffer (Wash buffer except containing 300 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The pA-Tn5 adapter complex containing 2.5 µl of 20x CUTANA™ pAG-Tn5 pre-loaded adapter complex (EpiCypher, #15-1017) was prepared in 50 µl of Dig-300 Buffer (20 mM HEPES pH 7.5 ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... (a step-by-step protocol https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3) using CUTANA™ pAG-Tn5 (Epicypher, 15-1017). Quantitative spike-in CUT&Tag was performed by adding Drosophila S2 cells at a 2:1 ratio to mouse spermatogonial cells (20,000 S2 cells to 10,000 mouse cells ...
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bioRxiv - Cancer Biology 2024Quote: CUT&RUN coupled with high-throughput DNA sequencing was performed on isolated nuclei using Cutana pA/G-MNase (Epicypher, 15-1016) according to the manufacturer’s manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed three times with digitonin wash buffer before resuspending in digitonin wash buffer with pAG-MNase (EpiCypher, 15-1116) at a concentration of 1:20 and nutating at 4°C for 1 hour to allow pAG-MNase and epitope binding ...
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bioRxiv - Developmental Biology 2022Quote: ... Two microliters of uniquely barcoded i5 and i7 primers (10 μM) and 25 μl CUTANA High Fidelity 2x PCR Master Mix (EpiCypher, #15-1018) were added to each sample and mixed ...
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bioRxiv - Molecular Biology 2023Quote: ... bead-cell complexes were washed twice with 200 μl cold dig-washing buffer (washing buffer supplemented with 0.01% digitonin) and resuspended in 50 μl cold dig-washing buffer with 1 μl pAG-MNase (EpiCypher, Cat #15-1016). After incubation at room temperature for 10 min ...