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Citations for EpiCypher :
51 - 100 of 129 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... or rabbit anti-human histone H3K4me3 polyclonal antibody (Epicypher #13-0041) as a positive control ...
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bioRxiv - Developmental Biology 2022Quote: ... Then 0.5 μg anti-rabbit IgG secondary antibody (EpiCypher, 13-0047) was added to each sample and incubated on a nutator for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... 1ul of antibody IgG (Rabbit IgG Negative Control, EpiCypher, 13-0042K), Pol II (Rpb1 CTD (4H8) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of Rabbit anti-H3K4me3 ChIP grade antibody (Epicypher, 13-0041), which has been SNAP-ChIP validated for H3K4me3 specificity 62 ...
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bioRxiv - Molecular Biology 2022Quote: Cut & Tag experiments were performed in duplicate using H3K4me3 antibody 13-0041 (EpiCypher) and H3K27ac antibody ab4729 (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rabbit antibody for CUTANA™ CUT&Tag Workflows (13-0047, EpiCypher).
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted with the primary antibodies against ADMA (Epicypher, SKU: 13-0011), SDMA (Millipore Cat# 07-413 ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed in Wash buffer 300 (Wash buffer containing 300mM NaCl) two times and resuspended in Wash buffer 300 containing Protein A/G-TN5 (EpiCypher) and incubated for an hour at RT ...
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bioRxiv - Cell Biology 2024Quote: ... and SDS-PAGE analysis with 17% polyacrylamide gel and Coomassie staining (0.12% (w/v) Coomassie G-250 in 20% methanol (v/v)) using known amounts of histone H3.1 recombinant protein (EpiCypher, Inc). Approximately 4 µg of histone octamer were mixed with an equal amount of heavy-isotope labelled histones ...
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bioRxiv - Cancer Biology 2021Quote: ... After overnight incubation at 4°C with the primary FOXA1 antibody (13-2001, Epicypher), cell-bead slurry was washed twice more after which pA-MNase digestion was activated by placing samples on an ice-cold block and incubated with digitonin wash buffer containing 2 mM CaCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% BSA) and 10 µL per antibody treatment of ConA-coated magnetic beads (Epicypher) added with gentle vortexing ...
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bioRxiv - Cell Biology 2023Quote: ... the following primary antibodies were used: anti-H3K9ac (rabbit, Epicypher 13-0033, 1:500), anti-γH2Av (mouse ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Digitonin and 0.5 μg of anti-rabbit or anti-mouse secondary antibodies (Epicypher). After incubation for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were washed in Dig-wash and incubated with ProteinA-MNase fusion protein at 700 ng/mL (EpiCypher, 15-1016-EPC) on a tube rotator at 4 °C for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: All histone markers antibodies for CUT&RUN were either certified by EpiCypher (H3K4me1, H3K4me3, H3K27me3) or validated by SNAP-ChIP qPCR (H3K27Ac) ...
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bioRxiv - Genetics 2023Quote: ... 2 μl /1×106 cells of the following antibodies were used for immunoprecipitations: H3K4me3 (EpiCypher 13-00041), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized using 0.01% digitonin and incubated with 0.5μg antibody (anti-Sox9 (CST, #82630) and IgG (# 13-0042; Epicypher) in 50μL antibody buffer (20mM HEPES at pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... & protease inhibitors) followed by the addition of 0.5 µg CUTANA anti-rabbit secondary antibody (#13-1047, EpiCypher). Samples were then incubated on nutator at room temperature for 1 hour to facilitate binding ...
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bioRxiv - Genetics 2023Quote: ... Heterochromatin formation or gene inactivation were detected using antibodies against H3K4me3 (0.5 µg/sample, EpiCypher #13-0041), H2K27me3 (0.5 µg/sample ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.5 µg of primary and secondary antibody were used with 1 µL of pA-Tn5 (Epicypher, 15-1017). Antibodies used ...
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bioRxiv - Genetics 2023Quote: ... Bead-bound cells were resuspended in 50 μl secondary antibody buffer (goat anti-rabbit IgG (Epicypher, 13-0047) diluted 1:100 in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... then resuspended and incubated at 4°C for 1 hour in CUT&RUN antibody buffer and 2.5 μL pAG-MNase (EpiCypher). ConA-bound-nuclei were then washed twice with CUT&RUN triton wash buffer ...
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bioRxiv - Genetics 2020Quote: ... We used immuno-tethered CUT&Tag chromatin profiling [Kaya-Okur et al, 2020] with antibodies to histone H3K27me3 (C36B11, Cell Signalling Technology) and to histone H3K4me2 (13-0027, Epicypher) modifications ...
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bioRxiv - Plant Biology 2024Quote: ... nuclei were resuspended in Antibody150 buffer and from antibody incubation to tagmentation with pAG-Tn5 (EpiCypher Cat No. 15-1017) we followed the CUT&Tag Protocol v1.7 from CUTANA™ ...
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bioRxiv - Biochemistry 2022Quote: ... Cell-bead solution was then buffer exchanged into Antibody Buffer and target-specific antibodies added to individual reaction tubes in the following amounts: 0.5 µg of anti-H3K4me3 (EpiCypher, 13-0041), 1 µg of anti-H3K27ac (EpiCypher ...
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bioRxiv - Genetics 2022Quote: ... containing 150 ng of DNA for leaf or 350 ng for ear was then diluted to a total volume of 1500 μL in 1X Wash Buffer and used as input for the CUT&Tag-IT Assay Kit with both anti-CENH3 (maize) antibodies (26) and IgG for each sample (Epicypher 13-0042). Libraries were amplified with 15 or 16 cycles of PCR ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies (FOXP1 Cell Signaling Technologies #2005S, FOXP2 Abcam #ab16046, FOXP4 Millipore #ABE74, H3K27me3 Cell Signaling Technologies #9733, IgG Epicypher #13-0042) were added at 1:50 dilution and samples were nutated overnight at 4C.
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bioRxiv - Cancer Biology 2023Quote: ... The reaction was quenched with STOP buffer and DNA was extracted with a DNA purification kit (Epicypher). Library preparation was conducted with the NEBnext Ultra II kit with the following protocol adjustments ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-H3K4me1 (13-0040) was from EpiCypher.
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bioRxiv - Genomics 2021Quote: ... The Tn5 was purchased from Epicypher (15-1017) and used at 1:20 ...
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bioRxiv - Cancer Biology 2022Quote: CUTnRUN was performed using CUTANA CUT&RUN kit (Epicypher) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... pAG-Tn5 was purchased from Epicypher (Durham, North Carolina), and H3K27me3 antibody was purchased from Cell Signalling Technologies (#9733) ...
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bioRxiv - Molecular Biology 2024Quote: ... Targeted chromatin digestion was achieved by pAG-MNase (EpiCypher) binding for 10 minutes at room temperature followed by incubation with CaCl2 at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Amplification of DNA was performed following guidelines outlined by EpiCypher: 98°C 45s ...
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bioRxiv - Molecular Biology 2023Quote: Cut&Run-seq was performed using CUTANA v3 kit (Epicypher) according to the manufacturer’s protocol and with mild crosslinking (1 min incubation with 1% formaldehyde at room temperature) ...
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bioRxiv - Genomics 2021Quote: CUT&Tag was performed using CUTANA™ CUT&Tag reagents (Epicypher, Durham NC ...
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN was performed using CUTANA™ Kit (EpiCypher #14-1048) with minor modifications according to the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... the DNA was purified using CUTANA DNA Purification Kit (EpiCypher, 14-0050) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: Cut&Run was performed using the CUTANA™ CUT&RUN Kit (Epicypher) on non-fixed nuclei according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... CUT&RUN for CEBPβ was performed using CUTANA CUT&RUN Kit (EpiCypher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher) using 260,000 to 500,000 cells/sample and the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Reaction was washed two additional times before pAG-MNAse (15-1016-EPC Epicypher) was added 1:20 in washing buffer and incubated for 1h at 4°C ...
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bioRxiv - Genomics 2023Quote: ... Free Widom601 dsDNA was amplified from a purchased template (cat. 18-0005, Epicypher) using primers 5’-CTGGAGAATCCCGGTGC and 5’-ACAGGATGTATATATCTGACACGTGC ...
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bioRxiv - Cancer Biology 2023Quote: CUT&TAG was conducted based on the CUTANA CUT&TAG protocol v1.5 (Epicypher). Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg ...
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN was performed based on the CUTANA CUT&RUN protocol v1.6 (Epicypher). Isolated nuclei from FT282 and KURAMOCHI were bound to Concalavin A beads and incubated overnight at 4℃ with primary antibody for H3K27me3 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... 400,000 microglia were used per sample and permeabilization was optimised using Digitonin (EpiCypher) 0.01% ...
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bioRxiv - Genomics 2023Quote: Widom601 dsDNA complexed with recombinant human histones was purchased from Epicypher (cat. 16-0009). Free Widom601 dsDNA was amplified from a purchased template (cat ...
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bioRxiv - Genomics 2022Quote: CUT&RUN on primary RPTEC culture was performed with CUTANA kit (EpiCypher, 14-1048) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: CUT&RUN was performed using the CUTANA CUT&RUN kit (#14-1048, EpiCypher, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: CUT&RUN was performed with the CUTANA ChIC/CUT&RUN kit (EpiCypher 14-1048) according to manufacturer’s instructions from manual version 3 ...