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51 - 59 of 59 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag E. coli since 2019

• Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion

  René Platzer, et al., bioRxiv - Immunology 2023

  Quote: ... featuring an unpaired cysteine residue and C-terminally extended with an AVI-tag (GLNDIFEAQKIEWHE) for site-specific biotinylation with the BirA biotin ligase (Avidity) preceded by a 3C protease recognition site (LEVLFQGP ...
• Development of LIBRA-seq for the Guinea Pig Model System as a Tool for the Evaluation of Antibody Responses to Multivalent HIV-1 Vaccines

  Matthew J. Vukovich, et al., bioRxiv - Immunology 2023

  Quote: Protein antigens used for LIBRA-seq and serum ELISA contained a C-terminal Avi-tag and were site specifically biotinylated using BirA biotin-protein ligase raction kit (Avidity) according to manufacturer’s instructions.
• Generation of circulating autoreactive pre-plasma cells fueled by naïve B cells in celiac disease

  Ida Lindeman, et al., bioRxiv - Immunology 2023

  Quote: ... coli lysates by Ni-NTA affinity chromatography followed by site-specific biotinylation using BirA biotin-protein ligase (Avidity). The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare ...
• SHP2 Inhibition Abrogates Adaptive Resistance to KRAS^G12C-Inhibition and Remodels the Tumor Microenvironment of KRAS-Mutant Tumors

  Carmine Fedele, et al., bioRxiv - Cancer Biology 2020

  Quote: Phage display vectors were converted into Fab expression vectors that contain a substrate tag for the biotin ligase BirA (AviTag, Avidity, LLC) at the carboxyl terminus of the heavy chain ...
• Single-Cell Profiling of the Antigen-Specific Response to BNT162b2 SARS-CoV-2 RNA Vaccine

  Kevin J. Kramer, et al., bioRxiv - Immunology 2021

  Quote: Constructs containing an Avi-tag (ZM197 Env and HA NC99) were biotinylated using the site-specific biotinylation kit according to manufacturer instructions (Avidity LLC.) All other antigens not containing an Avi-tag were non-specifically biotinylated using the EZ-Link Sulfo-NHS-Biotin kit at a 50:1 biotin:protein molar ratio.
• Design of amyloidogenic peptide traps

  Danny D. Sahtoe, et al., bioRxiv - Synthetic Biology 2023

  Quote: Proteins with Avi-tags (GLNDIFEAQKIEWHE; see supplementary materials) were purified as described above and biotinylated in vitro using the BirA500 (Avidity, LLC) biotinylation kit ...
• Von Willebrand factor A1 domain affinity for GPIbα and stability are differentially regulated by its O-glycosylated N-linker and C-linker

  Klaus Bonazza, et al., bioRxiv - Biophysics 2022

  Quote: The GPIbα used for kinetic measurements contained an N-terminal Avi-tag which was biotinylated using a BirA biotin-protein ligase kit (Cat #BirA500, Avidity, Aurora, CO). Biolayer interferometry (BLI ...
• How talin allosterically activates vinculin

  Florian Franz, et al., bioRxiv - Biophysics 2022

  Quote: ... pFN18a (Ig32)2-(R3IVVI)-(Ig32)2 was subcloned into a modified pFN18a vector engineered with the AviTagTM (Avidity) (sequence GLNDIFEAQKIEWHE) ...
• Simultaneous analysis of antigen-specific B and T cells after SARS-CoV-2 infection and vaccination

  Krista L. Newell, et al., bioRxiv - Immunology 2021

  Quote: ... spike protein probes were added one-by-one to FACS wash buffer (1x PBS, 2% fetal bovine serum) containing 5μM free d-biotin (Avidity, Cat. Bir500A). Both streptavidin-fluor conjugates were used to stain DMSO control samples to further verify the absence of significant frequencies of non-specific streptavidin-binding B cells.