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Citations for Avidity :
101 - 135 of 135 citations for Recombinant Human CEACAM8 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The RBD with a BirA tag was biotinylated using a biotinylation kit (Avidity, LLC) and was immobilized through the Biotin CAPture Reagent ...
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bioRxiv - Immunology 2024Quote: ... HLA-DR monomers were biotinylated overnight at 4°C using BirA biotin ligase (Avidity) and purified by size exclusion chromatography using Superdex 200 size exclusion column (AKTA ...
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bioRxiv - Immunology 2020Quote: ... we biotinylated SARS-CoV-2 RBD using a terminal AviTag and BirA biotin ligase (Avidity) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... I-Ek/ANP α-biotin was site-specifically biotinylated using the BirA biotin ligase (Avidity) and purified via Ni-NTA agarose (to remove BirA ...
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bioRxiv - Biophysics 2021Quote: ... Ecads bound to the Ni-NTA beads were biotinylated with BirA enzyme (BirA 500 kit; Avidity) for 1hr at 300C ...
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bioRxiv - Biophysics 2022Quote: ... Ecads bound to the Ni-NTA beads were biotinylated with BirA enzyme (BirA 500 kit; Avidity) for 1 h at 30 °C ...
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bioRxiv - Biophysics 2021Quote: Monomeric H57-scFV featuring a BirA recognition site was site-specifically biotinylated using a biotin ligase (Avidity) at 30 °C ...
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bioRxiv - Biophysics 2020Quote: ... M2β-S1 used for the in vitro motility assays was biotinylated with BirA (10 µg/ml, Avidity) as described ...
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bioRxiv - Biophysics 2023Quote: ... HA-PD1 with a C-terminal AviTag was biotinylated with BirA according to the manufacturer’s protocol (Avidity) and immobilized on a NeutrAvidin chip (Cytiva ...
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bioRxiv - Immunology 2019Quote: ... The pMHC or costimulatory ligand was then biotinylated in vitro by BirA enzyme according to the manufacturer’s instructions (Avidity) and purified using size-exclusion chromatography.
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bioRxiv - Immunology 2020Quote: The pMHC or costimulatory ligand was then biotinylated in vitro by BirA enzyme according to the manufacturer’s instructions (Avidity) and purified using size-exclusion chromatography with HBS-EP (0.01 M HEPES pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... into AviTag buffer and subsequently biotinylated with the BirA enzyme according to the manufacturer instructions (Avidity, Denver, Colorado, USA). Biotinylated MHC class II-peptide complexes were purified on a HisTrap HP column (Merck ...
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bioRxiv - Immunology 2019Quote: ... The generated peptide HLA class II complexes were biotinylated in a BirA sequence (DRB chain) using a BirA ligase (Avidity). The Biot-peptide-HLA class II complexes were then used to generate tetramers using Streptavidin-PE ...
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bioRxiv - Immunology 2022Quote: ... Secreted protein was purified using HisPur Ni-NTA resin (Thermo) for immobilized metal affinity chromatography (IMAC) and biotinylated overnight at 4°C using biotin (Avidity), ATP ...
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bioRxiv - Biophysics 2023Quote: ... The ‘Avi’ in the name mentions that this protein expresses an Avi-tag which allows the protein to be biotinylated in vivo of the bacterial cell with the help of biotin ligase enzymes co-expressed in a pBirAcm (Avidity Nanomedicine, La Jolla, CA) plasmid52 ...
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bioRxiv - Immunology 2020Quote: ... BirA biotinylation of S proteins was conducted using BirA biotin-protein ligase bulk reaction kit (Avidity). Coronavirus S proteins with Avi-tags were concentrated to 7-9 mg/ml using 100K Amicon tubes in TBS ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.325 μg/μl Maltose Binding Protein (MBP)-AviTag substrate (Avidity, L.L.C.), 8.3 mM ATP and 42 μM biotin ...
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bioRxiv - Immunology 2022Quote: ... Biotinylation was performed using a BirA biotin-protein ligation kit (Avidity) on proteins produced with a C-terminal avidin tag sequence ...
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bioRxiv - Microbiology 2020Quote: ... a BirA biotin-protein ligase kit (Avidity, LLC; Aurora, CO, USA) was used to biotinylate protein samples ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylation of the AviTag in full-length human FLCN-8xG-AviTag-PreScission-MBP was performed following according to the manufacturer’s (Avidity) suggested protocol and was performed following PreScission protease cleavage and prior to size exclusion chromatography ...
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bioRxiv - Immunology 2022Quote: ... Biotinylation of monomer was carried out using BirA biotin-protein ligase kit (Avidity) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Overnight biotinylation reactions were performed using the BirA Biotin-Protein Ligase Kit (Avidity) at 4℃ in 1x BiomixA ...
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bioRxiv - Immunology 2024Quote: ... biotinylation with BirA biotin-protein ligase standard reaction kit (Avidity, 318 LLC-Aurora, Colorado), and purification using size-exclusion column (Waters ...
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bioRxiv - Immunology 2023Quote: ... The two proteins were co-expressed with BirA contained in a pBirAcm vector (Avidity LLC) in E ...
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bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
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bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
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bioRxiv - Immunology 2023Quote: ... coli lysates by Ni-NTA affinity chromatography followed by site-specific biotinylation using BirA biotin-protein ligase (Avidity). The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylation was performed using the BirA biotin-protein ligase standard reaction kit (BirA-500) as per the manufacturer’s instructions (Avidity). This BG505 trimer probe was further purified by SEC on a HiLoad 16/600 Superdex 200 PG column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... 2 mL of the protein was added to 223 μL Biomix B and 5 μL (5 μg) BirA (both from Avidity) and rocked overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Biotinylation of AviTag-containing PyCSP[NXA] was performed using a BirA biotin-protein ligase kit (Avidity, LLC; Aurora, CO, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The site-specific biotinylation was then conducted by using BirA biotin-protein ligase standard reaction kit (Cat# BirA500) from Avidity Biosciences (Aurora ...
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bioRxiv - Immunology 2023Quote: ... HAPR8 tetramers for flow cytometry were generated by site-specific biotinylation of non-cysteine-stabilized treated HA protein containing the Y98F mutation that prevents sialic acid binding using BirA-500 ligase (Avidity), followed by Zeba desalting column purification (Thermo Fisher) ...
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bioRxiv - Biophysics 2021Quote: ... and the other end of the protein was decorated with a single paramagnetic bead through biotin-streptavidin chemistry (AviTag, Avidity Technology). Position of the permanent magnets ...
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bioRxiv - Immunology 2023Quote: ... Avi-tagged Env proteins at 25 µM were dialyzed into Tris pH8 and incubated for 5 hours with mild agitation at 30 °C using the BirA biotin-protein ligase reaction kit (Avidity LLC), then reconcentrated prior to size-exclusion chromatography ...
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bioRxiv - Biophysics 2021Quote: ... as hybrid proteins consisting of an N-terminal Ulp1-cleavable N-terminal His10-SUMO sequence followed by an AviTag sequence (Avidity, LCC, Aurora, Colorado, USA), facilitating in vivo biotinylation and four consecutive C-terminal Myc-tag sequences ...