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Citations for Avidity :
101 - 122 of 122 citations for Recombinant Human CD274 Biotin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Avi-tagged SARS-CoV-2 RBD and SD1-RBD (both corresponding to SARS-CoV-2 ancestral virus) were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... AviTagged Y2 COBRA HA proteins containing the Y98F mutation to reduce sialic acid binding were biotinylated using the BirA biotin-protein ligase in the BirA500 kit (Avidity) and complexed to streptavidin-fluorophores SA-PE (1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... Excess streptavidin was blocked with two 40 s (D52N STAR) or 60 s (D52N scFv) injections of 250 μM biotin (Avidity). Before injections of purified D52N ...
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bioRxiv - Molecular Biology 2021Quote: ... LacI with a C-terminal AviTag and biotin ligase were co-expressed in T7 Express Cells supplemented with 50 µM biotin from pET11a[LacR-Avi] and pBirAcm (Avidity) vectors ...
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bioRxiv - Immunology 2022Quote: ... Secreted protein was purified using HisPur Ni-NTA resin (Thermo) for immobilized metal affinity chromatography (IMAC) and biotinylated overnight at 4°C using biotin (Avidity), ATP ...
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bioRxiv - Immunology 2020Quote: ... Biotinylation of AviTag-containing PyCSP[NXA] was performed using a BirA biotin-protein ligase kit (Avidity, LLC; Aurora, CO, USA), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... with N-terminal MRGS(H)8 and C-terminal Avi tag was biotinylated in vivo by co-expressing biotin-ligase BirA (pBirAcm from Avidity) in E.coli BL21 (DE3) ...
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bioRxiv - Immunology 2023Quote: ... featuring an unpaired cysteine residue and C-terminally extended with an AVI-tag (GLNDIFEAQKIEWHE) for site-specific biotinylation with the BirA biotin ligase (Avidity) preceded by a 3C protease recognition site (LEVLFQGP ...
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bioRxiv - Immunology 2022Quote: Aliquots of RBD-HISAVI protein were biotinylated in vitro using the BirA500:BirA biotin-protein ligase standard reaction kit (Avidity) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The site-specific biotinylation was then conducted by using BirA biotin-protein ligase standard reaction kit (Cat# BirA500) from Avidity Biosciences (Aurora ...
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bioRxiv - Immunology 2023Quote: Protein antigens used for LIBRA-seq and serum ELISA contained a C-terminal Avi-tag and were site specifically biotinylated using BirA biotin-protein ligase raction kit (Avidity) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... spike protein probes were added one-by-one to FACS wash buffer (1x PBS, 2% fetal bovine serum) containing 5μM free d-biotin (Avidity, Cat. Bir500A). Both streptavidin-fluor conjugates were used to stain DMSO control samples to further verify the absence of significant frequencies of non-specific streptavidin-binding B cells.
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bioRxiv - Bioengineering 2019Quote: ... The recombinant extracellular domain of CD38 and PD-L1 was produced as a 6-His and AviTag™ fusion in HEK293 cells and purified by Ni-NTA followed by in vitro biotinylation by BirA biotin ligase (Avidity).
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bioRxiv - Cancer Biology 2020Quote: Phage display vectors were converted into Fab expression vectors that contain a substrate tag for the biotin ligase BirA (AviTag, Avidity, LLC) at the carboxyl terminus of the heavy chain ...
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bioRxiv - Biophysics 2021Quote: ... and the other end of the protein was decorated with a single paramagnetic bead through biotin-streptavidin chemistry (AviTag, Avidity Technology). Position of the permanent magnets ...
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bioRxiv - Biochemistry 2021Quote: ... 750 µg of purified uncleaved hSPAK-CCT or hOSR-CCT were mixed with 50 µl of SuperMix (containing ATP and D-biotin, Avidity, LLC) and 5 μg of BirA (Avidity ...
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bioRxiv - Immunology 2023Quote: ... Avi-tagged Env proteins at 25 µM were dialyzed into Tris pH8 and incubated for 5 hours with mild agitation at 30 °C using the BirA biotin-protein ligase reaction kit (Avidity LLC), then reconcentrated prior to size-exclusion chromatography ...
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bioRxiv - Biophysics 2022Quote: The GPIbα used for kinetic measurements contained an N-terminal Avi-tag which was biotinylated using a BirA biotin-protein ligase kit (Cat #BirA500, Avidity, Aurora, CO). Biolayer interferometry (BLI ...
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bioRxiv - Immunology 2021Quote: ... C-terminal Histidine/Avi-tagged) was obtained from BEI resources (Manassas, VA, USA, Cat. NR53524) and biotinylated using a BirA ubiquitin ligase (Avidity, Aurora, CO, USA, Cat. Bir500A) following the manufacturer’s recommended protocol ...
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bioRxiv - Biophysics 2023Quote: ... The ‘Avi’ in the name mentions that this protein expresses an Avi-tag which allows the protein to be biotinylated in vivo of the bacterial cell with the help of biotin ligase enzymes co-expressed in a pBirAcm (Avidity Nanomedicine, La Jolla, CA) plasmid52 ...
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bioRxiv - Immunology 2021Quote: To investigate Fc receptor binding recombinant Fc receptors with an AviTag were biotinylated using a Bir500 kit (Avidity, Aurora, CO, USA) according to manufacturer’s instructions and purified using a Zeba Spin Desalting Column ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylation of the AviTag in full-length human FLCN-8xG-AviTag-PreScission-MBP was performed following according to the manufacturer’s (Avidity) suggested protocol and was performed following PreScission protease cleavage and prior to size exclusion chromatography ...