Labshake search
Citations for Avidity :
51 - 100 of 134 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: Purified and Avi-tagged SARS-CoV-2 RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
-
bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 WT and Delta RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before(Robbiani et al. ...
-
bioRxiv - Immunology 2022Quote: ... soluble avidin-tagged Gamma S protein was biotinylated with a BIrA500 biotin-ligase reaction kit according to the manufacturer’s instruction (Avidity). Biotinylated protein was then mixed with streptavidin fluorophores (AF647 ...
-
bioRxiv - Immunology 2022Quote: ... Avi-tagged SARS-CoV-2 RBD and SD1-RBD (both corresponding to SARS-CoV-2 ancestral virus) were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... I-Ek/ANP α-biotin was site-specifically biotinylated using the BirA biotin ligase (Avidity) and purified via Ni-NTA agarose (to remove BirA ...
-
bioRxiv - Immunology 2021Quote: ... AviTagged Y2 COBRA HA proteins containing the Y98F mutation to reduce sialic acid binding were biotinylated using the BirA biotin-protein ligase in the BirA500 kit (Avidity) and complexed to streptavidin-fluorophores SA-PE (1:500 dilution ...
-
bioRxiv - Immunology 2020Quote: ... Biotinylation of AviTag-containing PyCSP[NXA] was performed using a BirA biotin-protein ligase kit (Avidity, LLC; Aurora, CO, USA), according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... The site-specific biotinylation was then conducted by using BirA biotin-protein ligase standard reaction kit (Cat# BirA500) from Avidity Biosciences (Aurora ...
-
bioRxiv - Immunology 2023Quote: Protein antigens used for LIBRA-seq and serum ELISA contained a C-terminal Avi-tag and were site specifically biotinylated using BirA biotin-protein ligase raction kit (Avidity) according to manufacturer’s instructions.
-
bioRxiv - Biophysics 2019Quote: ... d-Biotin was purchased from Avidity, LLC (Aurora ...
-
bioRxiv - Cell Biology 2023Quote: ... and 50 μM d-biotin (Avidity) at 4°C overnight.
-
bioRxiv - Biophysics 2021Quote: ... and the other end of the protein was decorated with a single paramagnetic bead through biotin-streptavidin chemistry (AviTag, Avidity Technology). Position of the permanent magnets ...
-
bioRxiv - Immunology 2023Quote: ... Avi-tagged Env proteins at 25 µM were dialyzed into Tris pH8 and incubated for 5 hours with mild agitation at 30 °C using the BirA biotin-protein ligase reaction kit (Avidity LLC), then reconcentrated prior to size-exclusion chromatography ...
-
bioRxiv - Biophysics 2020Quote: ... Excess streptavidin was blocked with biotin (Avidity). Before SHP-1 injection ...
-
bioRxiv - Biophysics 2022Quote: The GPIbα used for kinetic measurements contained an N-terminal Avi-tag which was biotinylated using a BirA biotin-protein ligase kit (Cat #BirA500, Avidity, Aurora, CO). Biolayer interferometry (BLI ...
-
bioRxiv - Immunology 2021Quote: ... D-biotin (20 μM; Avidity; Cat no I2011) was added 24 h after multimerization ...
-
bioRxiv - Biophysics 2019Quote: ... Biotin ligase BirA (Avidity LLC, Aurora, CO, US) was used to biotinylate Avitag-kinesin (28).
-
bioRxiv - Immunology 2020Quote: ... containing 5μM free d-biotin (Avidity, Cat# Bir500A). Free d-biotin ensured minimal cross-reactivity of antigen probes ...
-
bioRxiv - Biophysics 2022Quote: ... Constructs were biotinylated using BirA Biotin Ligase (Avidity) following the manufactures suggested protocol ...
-
bioRxiv - Immunology 2021Quote: ... Biotinylation was carried out with BirA biotin ligase (Avidity).
-
bioRxiv - Bioengineering 2022Quote: ... and 10 μL of 5 mM D-biotin (Avidity) were added to the protein along with BirA ligase ...
-
bioRxiv - Bioengineering 2022Quote: ... Purified samples were biotinylated using BirA biotin ligase (Avidity Inc.) at 1 µg of BirA enzyme per 10 nM of protein.
-
bioRxiv - Immunology 2021Quote: ... AviTagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
-
bioRxiv - Immunology 2021Quote: ... Avitagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
-
bioRxiv - Immunology 2022Quote: ... complexes were biotinylated overnight at 4°C using biotin (Avidity), ATP ...
-
bioRxiv - Immunology 2020Quote: ... Avitagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
-
bioRxiv - Immunology 2021Quote: ... for 10 min and excess streptavidin was quenched with 20 μM biotin (Avidity) for another 10 min ...
-
bioRxiv - Immunology 2022Quote: ... MHC multimers were diluted in PBS with 5.2 μM d-biotin (Avidity, Bio200) to 909 nM and incubated 20 min on ice ...
-
bioRxiv - Immunology 2023Quote: ... we extended the 14.4.4 scFV C-terminally with the BirA biotin ligase (Avidity) recognition site (GLNDIFEAQKIEWHE ...
-
bioRxiv - Immunology 2024Quote: ... Tetramers with an AviTag were biotinylated with the BirA biotin-ligase kit (Avidity) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... The HKU1 RBDs were biotinylated using the BirA biotin ligase reaction kit (Avidity) following the manufacturer’s protocol ...
-
bioRxiv - Genetics 2021Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity).
-
Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... Excess streptavidin was blocked with two 40 s injections of 500 µM biotin (Avidity) and the sensor was conditioned with at least 8 injections of running buffer ...
-
Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 µM biotin (Avidity). Before injections of soluble 1G4 or A6 αβTCR (51 kDa) ...
-
bioRxiv - Immunology 2021Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 µM biotin (Avidity). Before injections of soluble 1G4 or A6 αβTCR (51 kDa) ...
-
bioRxiv - Immunology 2020Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity). Before TCR injections ...
-
bioRxiv - Microbiology 2021Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity). Before RBD injections ...
-
bioRxiv - Immunology 2023Quote: ... Excess streptavidin was blocked with two 40 s injections of 250 μM biotin (Avidity). Before antibody injections ...
-
bioRxiv - Immunology 2024Quote: ... HLA-DR monomers were biotinylated overnight at 4°C using BirA biotin ligase (Avidity) and purified by size exclusion chromatography using Superdex 200 size exclusion column (AKTA ...
-
bioRxiv - Biophysics 2021Quote: ... coli BL21(DE3) cells harboring pBirAcm encoding the biotin ligase (Avidity, LCC, Aurora, Colorado, USA). For over-expression cells over-night cultures were diluted 1:100 in fresh LB medium supplemented with 20 mg/l Biotin ...
-
bioRxiv - Immunology 2020Quote: ... we biotinylated SARS-CoV-2 RBD using a terminal AviTag and BirA biotin ligase (Avidity) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2021Quote: Monomeric H57-scFV featuring a BirA recognition site was site-specifically biotinylated using a biotin ligase (Avidity) at 30 °C ...
-
bioRxiv - Immunology 2022Quote: ... and HA were prepared individually and mixed together after multimerization with 5uM free D-biotin (Avidity LLC) to minimize potential cross-reactivity between probes ...
-
bioRxiv - Immunology 2021Quote: ... and HA were prepared individually and mixed together after multimerization with 5uM free D-biotin (Avidity LLC) to minimize potential cross-reactivity between probes ...
-
bioRxiv - Immunology 2023Quote: ... Antigen probes were prepared individually and mixed together after multimerization with 5μM free D-biotin (Avidity LLC) to minimize potential cross-reactivity between probes ...
-
bioRxiv - Neuroscience 2023Quote: The amino terminal domain of GluA1 (1-394 a.a.) fused to a biotin acceptor tag (AviTag, Avidity) grown in suspension cultures of Sf9 cells was used as a target for the mRNA display selection ...
-
bioRxiv - Microbiology 2021Quote: ... the probe was generated by biotinylation of the GPΔmuc-WL2P2-1TD0-Avi trimer using biotin ligase BirA according to the manufacturer’s instructions (Avidity). Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare) ...
-
bioRxiv - Immunology 2020Quote: ... Avi-tagged HAs were expressed and purified as described above and biotinylated using the Biotin ligase kit (Avidity) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... the probes were generated by the biotinylation of Avi-tagged SARS-CoV-2 antigens using biotin ligase BirA according to the manufacturer’s instructions (Avidity). Biotin excess was removed by SEC on either a Superdex 200 10/300 column (GE Healthcare ...
-
bioRxiv - Immunology 2023Quote: ... HLA-I/peptide tetramer staining solution was prepared by mixing all tetramers into a solution of 1:1 FACS buffer and Brilliant Buffer containing 0.2% milk and 5μM free D-biotin (Avidity LLC) immediately prior to staining ...