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Citations for Avidity :
51 - 100 of 125 citations for Mouse E3 Ubiquitin Protein Ligase SMURF2 SMURF2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The site-specific biotinylation was then conducted by using BirA biotin-protein ligase standard reaction kit (Cat# BirA500) from Avidity Biosciences (Aurora ...
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bioRxiv - Immunology 2023Quote: ... Avi-tagged Env proteins at 25 µM were dialyzed into Tris pH8 and incubated for 5 hours with mild agitation at 30 °C using the BirA biotin-protein ligase reaction kit (Avidity LLC), then reconcentrated prior to size-exclusion chromatography ...
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bioRxiv - Immunology 2023Quote: ... purified spike ectodomain trimers and RBD domain protein were biotinylated by the addition of biotin-protein ligase (Avidity). Biotinylated proteins were buffer exchanged into PBS ...
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bioRxiv - Biophysics 2022Quote: The GPIbα used for kinetic measurements contained an N-terminal Avi-tag which was biotinylated using a BirA biotin-protein ligase kit (Cat #BirA500, Avidity, Aurora, CO). Biolayer interferometry (BLI ...
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bioRxiv - Immunology 2020Quote: ... purified spike ectodomain trimers were biotinylated by addition of biotin-protein ligase (Avidity, Aurora, CO). Biotinylated spike ectodomain trimers were buffer exchanged into PBS ...
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bioRxiv - Immunology 2020Quote: ... Biotinylation was performed using biotinprotein ligase standard reaction kit (Avidity, Cat# Bir500A) following the manufacturers standard protocol and dialyzed over-night against PBS ...
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bioRxiv - Biophysics 2024Quote: ... and subsequently biotinylated at the C-termini Avi tag sequence via BirA biotin-protein ligase (Avidity) (31) ...
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bioRxiv - Biochemistry 2022Quote: ... Biotinylation was performed using the BirA ligase reaction kit (Avidity, cat. no. BirA500).
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bioRxiv - Immunology 2024Quote: ... Tetramers with an AviTag were biotinylated with the BirA biotin-ligase kit (Avidity) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The HKU1 RBDs were biotinylated using the BirA biotin ligase reaction kit (Avidity) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: All antigens with an Avi tag were biotinylated enzymatically using BirA biotin-protein ligase (Avidity, Bulk BirA) while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... coli lysates by Ni-NTA affinity chromatography followed by site-specific biotinylation using BirA biotin-protein ligase (Avidity). The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... HAPR8 tetramers for flow cytometry were generated by site-specific biotinylation of non-cysteine-stabilized treated HA protein containing the Y98F mutation that prevents sialic acid binding using BirA-500 ligase (Avidity), followed by Zeba desalting column purification (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: ... Avi-tagged HAs were expressed and purified as described above and biotinylated using the Biotin ligase kit (Avidity) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... Biotin ligase BirA (Avidity LLC, Aurora, CO, US) was used to biotinylate Avitag-kinesin (28).
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bioRxiv - Biophysics 2022Quote: ... Constructs were biotinylated using BirA Biotin Ligase (Avidity) following the manufactures suggested protocol ...
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bioRxiv - Immunology 2021Quote: ... Biotinylation was carried out with BirA biotin ligase (Avidity).
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bioRxiv - Bioengineering 2022Quote: ... Purified samples were biotinylated using BirA biotin ligase (Avidity Inc.) at 1 µg of BirA enzyme per 10 nM of protein.
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bioRxiv - Immunology 2021Quote: ... AviTagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
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bioRxiv - Immunology 2021Quote: ... Avitagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
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bioRxiv - Immunology 2020Quote: ... Avitagged antigens were biotinylated using BirA biotin ligase (Avidity LLC).
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bioRxiv - Immunology 2022Quote: ... BG505.SOSIP.664.Avi was biotinylated using the BirA-Ligase (Avidity) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Biotinylation was performed using a BirA biotin-protein ligation kit (Avidity) on proteins produced with a C-terminal avidin tag sequence ...
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bioRxiv - Biophysics 2019Quote: ... Monomeric PS-CFP2 was site-specifically biotinylated using the BirA ligase (Avidity) to obtain the molecular probe biotin-PS-CFP2 ...
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bioRxiv - Immunology 2023Quote: ... we extended the 14.4.4 scFV C-terminally with the BirA biotin ligase (Avidity) recognition site (GLNDIFEAQKIEWHE ...
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bioRxiv - Bioengineering 2021Quote: The purified protein was biotinylated by BirA using a BirA-500 kit (Avidity). Typically ...
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bioRxiv - Microbiology 2021Quote: ... Purified SARS2 D614G spike protein was biotinylated using BirA500 biotinylation kit from Avidity. To 50 ug of spike protein ...
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bioRxiv - Immunology 2024Quote: ... HLA-DR monomers were biotinylated overnight at 4°C using BirA biotin ligase (Avidity) and purified by size exclusion chromatography using Superdex 200 size exclusion column (AKTA ...
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bioRxiv - Biophysics 2021Quote: ... coli BL21(DE3) cells harboring pBirAcm encoding the biotin ligase (Avidity, LCC, Aurora, Colorado, USA). For over-expression cells over-night cultures were diluted 1:100 in fresh LB medium supplemented with 20 mg/l Biotin ...
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bioRxiv - Immunology 2020Quote: ... we biotinylated SARS-CoV-2 RBD using a terminal AviTag and BirA biotin ligase (Avidity) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... I-Ek/ANP α-biotin was site-specifically biotinylated using the BirA biotin ligase (Avidity) and purified via Ni-NTA agarose (to remove BirA ...
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bioRxiv - Biophysics 2021Quote: Monomeric H57-scFV featuring a BirA recognition site was site-specifically biotinylated using a biotin ligase (Avidity) at 30 °C ...
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bioRxiv - Biophysics 2019Quote: ... was C-terminally equipped with an AVI-tag (GLNDIFEAQKIEWHE) for site-specific biotinylation via the BirA ligase (Avidity) followed by a 3C protease cleavable (LEVLFQGP ...
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bioRxiv - Microbiology 2021Quote: ... the probe was generated by biotinylation of the GPΔmuc-WL2P2-1TD0-Avi trimer using biotin ligase BirA according to the manufacturer’s instructions (Avidity). Biotin excess was removed by SEC on a Superdex 200 10/300 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins bound to Ni-NTA were biotinylated by incubating the beads with BirA enzyme (BirA500 kit; Avidity) for 1hr in 30 °C followed by an overnight incubation at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... the probes were generated by the biotinylation of Avi-tagged SARS-CoV-2 antigens using biotin ligase BirA according to the manufacturer’s instructions (Avidity). Biotin excess was removed by SEC on either a Superdex 200 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Purified proteins with an avi-tag were biotinylated by using BirA following the BirA500 kit’s protocol (Avidity, BirA500). Biotinylation was confirmed by performing a Coomassie gel shift assay according to Fairhead and Howarth ...
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bioRxiv - Molecular Biology 2021Quote: ... LacI with a C-terminal AviTag and biotin ligase were co-expressed in T7 Express Cells supplemented with 50 µM biotin from pET11a[LacR-Avi] and pBirAcm (Avidity) vectors ...
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bioRxiv - Immunology 2019Quote: ... The generated peptide HLA class II complexes were biotinylated in a BirA sequence (DRB chain) using a BirA ligase (Avidity). The Biot-peptide-HLA class II complexes were then used to generate tetramers using Streptavidin-PE ...
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bioRxiv - Microbiology 2019Quote: ... E2mc3 was generated by biotinylation of the individual Avi-tagged HCV E2mc3 using biotin ligase BirA according to the manufacturer’s instructions (Avidity LLC). Biotin excess was removed by SEC on a Superdex 200 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... with N-terminal MRGS(H)8 and C-terminal Avi tag was biotinylated in vivo by co-expressing biotin-ligase BirA (pBirAcm from Avidity) in E.coli BL21 (DE3) ...
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bioRxiv - Immunology 2023Quote: ... featuring an unpaired cysteine residue and C-terminally extended with an AVI-tag (GLNDIFEAQKIEWHE) for site-specific biotinylation with the BirA biotin ligase (Avidity) preceded by a 3C protease recognition site (LEVLFQGP ...
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bioRxiv - Immunology 2021Quote: ... The captured SARS-CoV-2 protein was then biotinylated using the BIRA500 kit (∼2.5 μg per 10 nmol AVI substrate) (Avidity) and cleaved from the Fc purification tag concurrently with 200 μg of HRV3C prepared as described [42] ...
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bioRxiv - Bioengineering 2019Quote: ... The recombinant extracellular domain of CD38 and PD-L1 was produced as a 6-His and AviTag™ fusion in HEK293 cells and purified by Ni-NTA followed by in vitro biotinylation by BirA biotin ligase (Avidity).
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bioRxiv - Cancer Biology 2020Quote: Phage display vectors were converted into Fab expression vectors that contain a substrate tag for the biotin ligase BirA (AviTag, Avidity, LLC) at the carboxyl terminus of the heavy chain ...
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bioRxiv - Biophysics 2023Quote: ... The ‘Avi’ in the name mentions that this protein expresses an Avi-tag which allows the protein to be biotinylated in vivo of the bacterial cell with the help of biotin ligase enzymes co-expressed in a pBirAcm (Avidity Nanomedicine, La Jolla, CA) plasmid52 ...
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bioRxiv - Molecular Biology 2021Quote: Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
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bioRxiv - Immunology 2020Quote: S protein was biotinylated using Bir-A (Avidity) and labelled by the sequential addition of streptavidin (SA ...
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bioRxiv - Biophysics 2022Quote: ... with biotinylated/unbiotinulated MBP-AviTagTM fusion protein (Avidity) as controls.
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bioRxiv - Immunology 2021Quote: ... and HA proteins were biotinylated using AviTag technology (Avidity) according to published protocols (51 ...