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Citations for Avidity :
51 - 91 of 91 citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Avi-tagged proteins were biotinylated using the biotinylated by BirA enzymatic reaction (Avidity, Inc) according to the manufacturer’s protocol and purified by SEC ...
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bioRxiv - Biophysics 2019Quote: ... was C-terminally equipped with an AVI-tag (GLNDIFEAQKIEWHE) for site-specific biotinylation via the BirA ligase (Avidity) followed by a 3C protease cleavable (LEVLFQGP ...
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bioRxiv - Immunology 2022Quote: ... VH F6 was biotinylated with BirA biotin-protein ligase standard reaction kit (BirA500, Avidity, USA). Streptavidin biosensors (ForteBio ...
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bioRxiv - Immunology 2020Quote: ... purified spike ectodomain trimers were biotinylated by addition of biotin-protein ligase (Avidity, Aurora, CO). Biotinylated spike ectodomain trimers were buffer exchanged into PBS ...
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bioRxiv - Immunology 2022Quote: ... The Protein A resin captured AVI-tagged ZM197-ZM233V1V2 was biotinylated with BirA enzyme (Avidity) and cleaved from the Fc purification tag with HRV3C protease ...
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bioRxiv - Immunology 2023Quote: ... The two proteins were co-expressed with BirA contained in a pBirAcm vector (Avidity LLC) in E ...
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bioRxiv - Immunology 2021Quote: ... trimers used as baits in flow cytometry were expressed with a C-term Avi-tag and biotinylated using a BirA biotinylation kit according to manufacturer’s instructions (Avidity). The BG505 MD39-base KO trimer had the following mutations relative to the BG505 MD39 SOSIP ...
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bioRxiv - Immunology 2022Quote: ... Secreted protein was purified using HisPur Ni-NTA resin (Thermo) for immobilized metal affinity chromatography (IMAC) and biotinylated overnight at 4°C using biotin (Avidity), ATP ...
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bioRxiv - Cell Biology 2022Quote: ... with N-terminal MRGS(H)8 and C-terminal Avi tag was biotinylated in vivo by co-expressing biotin-ligase BirA (pBirAcm from Avidity) in E.coli BL21 (DE3) ...
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bioRxiv - Immunology 2023Quote: ... featuring an unpaired cysteine residue and C-terminally extended with an AVI-tag (GLNDIFEAQKIEWHE) for site-specific biotinylation with the BirA biotin ligase (Avidity) preceded by a 3C protease recognition site (LEVLFQGP ...
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bioRxiv - Microbiology 2021Quote: ... TGEV and CCoV-HuPn-2018 were biotinylated using BirA biotin-protein ligase standard reaction kit (Avidity) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
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bioRxiv - Bioengineering 2021Quote: ... Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity). Next ...
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bioRxiv - Microbiology 2021Quote: ... The samples were then biotinylated in vivo using a BirA biotin-protein ligase standard reaction kit (Avidity). Following the kit instructions ...
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bioRxiv - Immunology 2020Quote: All antigens with an Avi tag were biotinylated enzymatically using BirA biotin-protein ligase (Avidity, Bulk BirA) while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins bound to Ni-NTA were biotinylated by incubating the beads with BirA enzyme (BirA500 kit; Avidity) for 1hr in 30 °C followed by an overnight incubation at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Purified proteins with an avi-tag were biotinylated by using BirA following the BirA500 kit’s protocol (Avidity, BirA500). Biotinylation was confirmed by performing a Coomassie gel shift assay according to Fairhead and Howarth ...
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bioRxiv - Biophysics 2021Quote: ... The protein was in-vivo biotinylated using BirA produced from the pBirAcm plasmid (Avidity LCC, Aurora, CO, USA). Expression of the GRLBD-encoding gene was induced with 0.5 mM IPTG at 15°C for 16 hours ...
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bioRxiv - Molecular Biology 2023Quote: Purified mini-HA was biotinylated using the Biotin-Protein Ligase-BIRA kit according to the manufacturer’s instructions (Avidity). Biotinylated mini-HA was then conjugated to streptavidin– PE (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... avi-tagged proteins were biotinylated with a BirA500 biotin-ligase reaction kit according to the manufacturer’s instruction (Avidity). TT was purchased from Creative Biolabs (Vcar-Lsx003) ...
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bioRxiv - Immunology 2023Quote: ... coli lysates by Ni-NTA affinity chromatography followed by site-specific biotinylation using BirA biotin-protein ligase (Avidity). The biotinylated proteins were further purified by anion-exchange chromatography using a Mono Q column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: Purified and Avi-tagged SARS-CoV-2 RBD or SARS-CoV-2 RBD KEN mutant (K417N, E484K, N501Y) was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before 3 ...
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bioRxiv - Immunology 2021Quote: Purified and Avi-tagged SARS-CoV-2 RBD or SARS-CoV-2 RBD K417N/E484K/N501Y mutant was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before3 ...
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bioRxiv - Immunology 2020Quote: ... Folded MHC-I molecules were biotinylated using the BirA biotin-protein ligase standard reaction kit (Avidity, LLC-Aurora, Colorado), and MHC-I monomers were purified using size exclusion chromatography (HPLC ...
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bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 Wuhan-Hu-1 RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before18 ...
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bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 NTD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before (Robbiani et al. ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylation was performed using the BirA biotin-protein ligase standard reaction kit (BirA-500) as per the manufacturer’s instructions (Avidity). This BG505 trimer probe was further purified by SEC on a HiLoad 16/600 Superdex 200 PG column (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 Wuhan-Hu-1 RBD and NTD were biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before (11) ...
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bioRxiv - Immunology 2021Quote: Purified and Avi-tagged SARS-CoV-2 RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before 1 ...
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bioRxiv - Immunology 2021Quote: ... The captured SARS-CoV-2 protein was then biotinylated using the BIRA500 kit (∼2.5 μg per 10 nmol AVI substrate) (Avidity) and cleaved from the Fc purification tag concurrently with 200 μg of HRV3C prepared as described [42] ...
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bioRxiv - Immunology 2020Quote: Purified and Avi-tagged SARS-CoV-2 RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity). Ovalbumin (Sigma ...
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bioRxiv - Immunology 2022Quote: Purified and Avi-tagged SARS-CoV-2 WT and Delta RBD was biotinylated using the Biotin-Protein Ligase-BIRA kit according to manufacturer’s instructions (Avidity) as described before(Robbiani et al. ...
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bioRxiv - Immunology 2022Quote: ... soluble avidin-tagged Gamma S protein was biotinylated with a BIrA500 biotin-ligase reaction kit according to the manufacturer’s instruction (Avidity). Biotinylated protein was then mixed with streptavidin fluorophores (AF647 ...
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bioRxiv - Immunology 2021Quote: ... AviTagged Y2 COBRA HA proteins containing the Y98F mutation to reduce sialic acid binding were biotinylated using the BirA biotin-protein ligase in the BirA500 kit (Avidity) and complexed to streptavidin-fluorophores SA-PE (1:500 dilution ...
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bioRxiv - Biochemistry 2019Quote: ... 2 mL of the protein was added to 223 μL Biomix B and 5 μL (5 μg) BirA (both from Avidity) and rocked overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Biotinylation of AviTag-containing PyCSP[NXA] was performed using a BirA biotin-protein ligase kit (Avidity, LLC; Aurora, CO, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The site-specific biotinylation was then conducted by using BirA biotin-protein ligase standard reaction kit (Cat# BirA500) from Avidity Biosciences (Aurora ...
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bioRxiv - Immunology 2023Quote: ... HAPR8 tetramers for flow cytometry were generated by site-specific biotinylation of non-cysteine-stabilized treated HA protein containing the Y98F mutation that prevents sialic acid binding using BirA-500 ligase (Avidity), followed by Zeba desalting column purification (Thermo Fisher) ...
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bioRxiv - Biophysics 2021Quote: ... and the other end of the protein was decorated with a single paramagnetic bead through biotin-streptavidin chemistry (AviTag, Avidity Technology). Position of the permanent magnets ...
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bioRxiv - Biophysics 2022Quote: The GPIbα used for kinetic measurements contained an N-terminal Avi-tag which was biotinylated using a BirA biotin-protein ligase kit (Cat #BirA500, Avidity, Aurora, CO). Biolayer interferometry (BLI ...
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bioRxiv - Biophysics 2021Quote: ... as hybrid proteins consisting of an N-terminal Ulp1-cleavable N-terminal His10-SUMO sequence followed by an AviTag sequence (Avidity, LCC, Aurora, Colorado, USA), facilitating in vivo biotinylation and four consecutive C-terminal Myc-tag sequences ...