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Citations for KeraFAST :
1 - 50 of 69 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... and for recombinant protein expression E .coli Lobstr cells (Kerafast EC1002) were used respectively ...
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bioRxiv - Microbiology 2020Quote: ... and glycoprotein (G) proteins (courtesy of Dr. Michael Whitt; KeraFAST) (53) ...
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bioRxiv - Molecular Biology 2019Quote: ... coli LOBSTR (Kerafast) cells following cold shock and induction with 0.5 mM IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Kerafast) were transformed with the expression vector and grown at 37 °C in LB medium with 0.4% (w/v ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli LOBSTR (Kerafast) cells and plated on LB ampicillin (100 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... coli cells (Kerafast) and Get1 was expressed in E ...
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bioRxiv - Immunology 2019Quote: ... anti-Zika E antibody (Kerafast) was used ...
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bioRxiv - Cell Biology 2023Quote: ... coli LOBSTR cells (Kerafast), and grown in 2XYT media supplemented with 10 μM ZnCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... coli LOBSTR strain (Kerafast) in TB medium supplemented with kanamycin (50 µg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... coli LOBSTR expression strain (Kerafast). Cells were grown overnight at 18°C in TB medium and expression was induced with 0.05 mM of IPTG at OD260nm = 0.8-1.0 ...
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bioRxiv - Microbiology 2021Quote: ... coli LOBSTR-BL21(DE3) (Kerafast) containing pET29b-DpaA-his (encoding PelB-DpaA(20-246)-His6 ...
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bioRxiv - Biochemistry 2021Quote: ... coli LOBSTR cells (EC1001, KeraFast) were used for the expression of PRAS40 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli expression strains (Kerafast, EC1002) were transformed ...
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bioRxiv - Microbiology 2024Quote: ... coli LOBSTR strain (Kerafast EC1002). Cell pellets from 50 ml E ...
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bioRxiv - Immunology 2023Quote: ... and the pcDNA3.1-spike protein plasmids and G*ΔG-VSV (Kerafast) with firefly luciferase in place of VSV-G were transfected into 293T cells (American Type Culture Collection [ATCC] ...
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bioRxiv - Neuroscience 2021Quote: ... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were infected at a multiplicity of infection of 3-5 for 2 hours at 37°C with rVSV-ΔG pseudovirus complemented in trans with the G glycoprotein and bearing a luciferase gene (Kerafast). Cells were then washed 6 times with DMEM + 10% FBS and returned to culture ...
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bioRxiv - Microbiology 2023Quote: ... a BlueScript backbone plasmid under T7 polymerase promoter control encoding VSV Indiana backbone in which the VSV glycoprotein (G) gene has been replaced with GFP (pVSV-ΔG-GFP) was purchased from Kerafast. The pVSV-ΔG-GFP was modified by cloning the codon optimized ORF of RABV G protein of Egyptian strain (GenBank accession number MK760770.1 ...
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bioRxiv - Biochemistry 2020Quote: ... coli LOBSTR-RIL(DE3)53 (Kerafast) cells and protein production was induced with 0.2 mM IPTG at 18 °C for 12-14 h ...
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bioRxiv - Microbiology 2021Quote: ... coli Lobstr BL21 (DE3) RIL (Kerafast), and protein was produced in the M9 media ...
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bioRxiv - Biophysics 2019Quote: Escherichia coli LOBSTR-RIL(DE3) (Kerafast)43 cells were co-transformed with vectors ...
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bioRxiv - Biochemistry 2019Quote: ... coli strain LOBSTR(DE3) RIL (Kerafast) 46 was transformed with the MBP-TorsinA construct ...
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bioRxiv - Microbiology 2021Quote: ... coli LOBSTR-BL21(DE3) cells (Kerafast) harbouring the pET28a-LdtE-his plasmid were grown in 2 L of LB-autoinduction medium (LB supplemented with 0.5% glycerol ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC-CBM ...
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bioRxiv - Biochemistry 2022Quote: ... coli expression strain LOBSTR (Kerafast, #EC1001) to minimize purification of endogenous Ni(II ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21(DE3) LOBSTR cells (Kerafast) to generate the various NEC oligomeric and heterodimeric interface mutants (listed in Supplementary Table S18) ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21(DE3) LoBSTr cells (Kerafast) to produce the NEC-SUPUL31 and NEC-DNUL34/SUPUL31 crystallization constructs ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220-His8 ...
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bioRxiv - Biochemistry 2019Quote: ... coli BL21 (DE3) (Kerafast Inc, Boston, MA) cells transformed with DDX21 expressing pET28b ...
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bioRxiv - Biochemistry 2021Quote: ... coli LOBSTR-BL21 (DE3) RIL (Kerafast Inc) using a pET26b-derived expression vector (81) ...
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bioRxiv - Biochemistry 2022Quote: ... coli (Kerafast, Winston-Salem, NC, Ref: EC1002) transformed via heat shock with plasmids validated by sequencing ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21(DE3) LOBSTR cells (Kerafast EC1002) were employed for recombinant protein expression and purification ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 293T/Spike cells were incubated with pseudotyped G*ΔG-GFP rVSV (Kerafast EH1024-PM) at MOI ∼3 for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... coli strains DH5α and LOBSTR-BL21(DE3) (Kerafast, #EC1002). NikABCDE was cloned into the first site ...
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bioRxiv - Cell Biology 2021Quote: ... coli expression strain LOBSTR (Andersen et al., 2013) (Kerafast, Boston, MA) transformed with pTf16 ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was generated by nucleofection of BHK cells (maintained in DMEM/F12 with 10%FBS and 5%TPB) with Spike-expressing plasmid followed by transduction with G-pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV (Kerafast) 18-24 hours later ...
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bioRxiv - Biochemistry 2020Quote: The Nup84-Nup133CTD vector was transformed into Escherichia coli LOBSTR-RIL(DE3) (Kerafast)68 cells and protein production was induced with 0.2 mM IPTG at 18 °C for 12-14 h ...
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bioRxiv - Immunology 2023Quote: ... CRL-3216) was transfected with the spike protein-expressing plasmids and then infected with G*ΔG-VSV virus (Kerafast, EH1020-PM). After culturing ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (Bigalke et al ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (NEC construct absent of the UL34 C-terminal transmembrane helix)14 ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Microbiology 2021Quote: ... or BHK-21/WI-2 (Kerafast) cells were transfected with SARS-CoV-2 Spike expression plasmid (pTT5 SARS-CoV-2 SΔ21) ...
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bioRxiv - Microbiology 2022Quote: ... BHK21/WI-2 cells (Kerafast #EH1011) were maintained in DMEM (Thermo Fisher #11965092 ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S-expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described.6 For the neutralization assay ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S expression plasmid and subsequently infected with VSV△G-firefly-luciferase as previously described [40] ...
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bioRxiv - Immunology 2021Quote: ... BHK21/WI-2 cells (Kerafast, EH1011) were transfected with the Wuhan-1 strain (Genbank # ...