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Citations for KeraFAST :
101 - 139 of 139 citations for Mouse IgG2a Isotype Control Antibody Biotin 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Immunoglobulin (Ig) genes of the three hybridomas were then sequenced (Absolute Antibody/Kerafast, Boston MA USA) using Next Generation Sequencing (NGS ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies include the following: rabbit-anti-eEF1A ([1:10,000 - IB], ED7001, Kerafast Inc., Boston, MA), mouse-anti-FLAG M2 ([1:2000-1:5000 - IB] ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was incubated with a dilution series of mouse serum (dilutions as indicated) and anti-VSV-G (Kerafast; 1 μg/mL) antibody for 30 minutes at room temperature and added to the HEK-Blue 293 hACE2-TMPRSS2 cells ...
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bioRxiv - Microbiology 2022Quote: ... 34850, 1:2,000 dilution) (rabbit anti-ORF8, GeneTex, Cat #: GTX135591, 1:1,000 dilution) (mouse anti-VSV-M, Kerafast, Cat #: EB0011, 1:100,000 dilution), prepared in WB blocking buffer (5% skim milk (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... membranes were stained with Coomassie brilliant blue and then hybridized with an anti puromycin antibody (Kerafast, #EQ0001) to detect puromycinylated polypetides.
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bioRxiv - Microbiology 2022Quote: ... Viral titer was evaluated as FFU/mL using the anti-VSV-Matrix protein 23H12 antibody (Kerafast Inc.) to detect infected cells.
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bioRxiv - Microbiology 2019Quote: ... cells were incubated overnight at 4°C with anti-M2 monoclonal antibody (E10; catalog no. EMS009, Kerafast). Three washes were performed prior to addition of goat anti-mouse Alexa Fluor-647 secondary antibody (catalog no ...
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bioRxiv - Microbiology 2021Quote: ... VSV M protein was detected using anti-VSV M antibody clone [23H12] (KeraFast, Boston, MA, Cat.# EB0011) at 1:1000 ...
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bioRxiv - Genomics 2022Quote: ... 275 mM NaCl) and incubated overnight at 4°C in the presence of 1.5 µg of S9.6 purified antibody (Kerafast). Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA–RNA hybrid [S9.6] antibody (Kerafast) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and cells were washed with PBS before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralise residual input virus ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoprecipitation was carried out in 1X DRIP binding buffer (0.02% Tween 20 in 1X PBS) with 11 μg of S9.6 antibody (Kerafast) per reaction overnight at 4°C with constant rotation ...
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bioRxiv - Cancer Biology 2020Quote: R-loops were quantified based on the total nuclear intensity of the S9.6 antibody (Kerafast Cat# ENH001, RRID:AB_2687463). Cells were imaged with a Leica microscope using 40x and 63x objectives ...
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bioRxiv - Neuroscience 2020Quote: ... and protein synthesis level was determined by Western blot using anti-puromycin antibody (KeraFAST, Cat#EQ0001, 1:1000). The combined intensity of proteins ranging from 20 to 200 kDa was quantified and normalized to the level of β-actin.
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bioRxiv - Plant Biology 2023Quote: ... then incubated with a 1:100 dilution of LM20 or LM19 primary antibody (Kerafast, ELD003 and ELD001 respectively) (Verhertbruggen et al. ...
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bioRxiv - Cell Biology 2022Quote: ... RNase H treated and non-treated samples were incubated with 10 μl of the S9.6 antibody (Kerafast ENH001) for 16 h at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA/DNA hybrids were immunoprecipitated from digested DNA (4 μg) using the S9.6 (10 μg) antibody (Kerafast EHN001), or mouse IgG antibody as negative control ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.0/140 mM NaCl/0.05% Triton X-100) and 5 μg of the S9.6 antibody (Kerafast, 1mg/ml) or 5 μg of the BrdU antibody (#555627 ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight on a rotating wheel at 4 °C in presence of 1 μg of S9.6 antibody (Kerafast) and 20 μl of protein G sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The blocking solution was removed and replaced with fresh TBST containing 5% (w/v) milk and S9.6 anti-DNA-RNA Hybrid Antibody (Kerafast) at 1μg/ml and incubated at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... Total cell lysates were generated and subjected to SDS-PAGE and immunoblot with an anti-puromycin antibody (Kerafast, EQ0001).
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bioRxiv - Biochemistry 2019Quote: Global nuclear R-loop detection was ascertained via R-loop immunofluorescence using S9.6 antibody for R-loop (Kerafast, ENH001). 24 h prior to immunofluorescence ...
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bioRxiv - Microbiology 2021Quote: ... Cells were subsequently washed five times and supplied with fresh DMEM + 10% FBS medium + anti-VSV-G antibody (Kerafast). Cell-free supernatants were harvested at 24 h after transduction ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg chromatin was used for immunoprecipitation (IP) reactions in 100 µL aliquots with 5 µg S9.6 antibody (Kerafast), as per the kit protocol ...
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bioRxiv - Genomics 2023Quote: The specificity of the S9.6 antibody for RNA/DNA hybrids was confirmed via dot blot using a protocol provided by Kerafast, which generates a commercially available S9.6 antibody ...
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bioRxiv - Genomics 2019Quote: ... Antibodies used for immunostaining include the following: primary antibodies (anti-γH2A.X, Cell Signaling, 1:500; anti-FMRP, Cell signaling, 1:200; S9.6, Kerafast, 1:500 for lymphoblasts and 1:120 for fibroblasts ...
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bioRxiv - Immunology 2020Quote: ... after which medium was removed and cells were washed with PBS before complete medium supplemented with anti-VSV-G antibody (8G5F11, Kerafast) was added to the cells ...
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bioRxiv - Cell Biology 2022Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA-RNA hybrid [S9.6] antibody (Kerafast, 1:1000) overnight at 4°C ...
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bioRxiv - Pathology 2019Quote: R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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bioRxiv - Immunology 2020Quote: ... the inoculum was removed and cells were washed with PBS before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralise residual input virus ...
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bioRxiv - Physiology 2020Quote: In vivo muscle protein synthesis was assessed using the non-isotopic SUnSET method and an antibody against puromycin (Kerafast, Boston, MA), as described previously [31 ...
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bioRxiv - Physiology 2020Quote: ... Protein synthesis was determined in the freely fed state using the SUnSET method and an antibody against puromycin (Kerafast, Boston, MA)25,26 ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with phosphate buffered saline (PBS) before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralize residual VSV-G-complemented input virus ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with phosphate buffered saline (PBS) before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralize residual VSV-G-complemented input virus ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were gently washed three times 2-hour post-infection and incubated in DMEM containing 1:1000 dilution of anti-VSV-G antibody (Kerafast: 8G5F11) to eliminate residual ΔG-VSV particles at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Sections were treated with blocking solution containing 3% (w/v) skim milk in PBS for 30 min and then with LM5 antibody (Kerafast, USA) diluted 5-fold in blocking solution for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... After 1.5 h at 37°C cells were washed with PBS and incubated with DMEM containing 1 μg/ml anti-VSV-G antibody (Kerafast, EB0010) for 24 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was replenished with DMEM+10%FBS containing a 1:20,000 dilution of an anti-VSV-G antibody 8G5F11 (Kerafast Cat. No EB0010), which reduces any possible background from residual VSV-G ...