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Citations for KeraFAST :
51 - 100 of 181 citations for Cow G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... together with Anti-Influenza A Non-Structural Protein 1 (NS1) Mouse Monoclonal Antibody (EMB005, Kerafast). Cells were then washed and incubated with a mixture of secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with VSV(G*ΔG-luciferase) (Kerafast) at an MOI of 3 infectious units/cell ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was replenished with DMEM+10%FBS containing a 1:20,000 dilution of an anti-VSV-G antibody 8G5F11 (Kerafast Cat. No EB0010), which reduces any possible background from residual VSV-G ...
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bioRxiv - Microbiology 2021Quote: ... transfected cells were infected with VSV(G*ΔG-luciferase) (Kerafast) at an MOI of 3 infectious units/cell ...
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bioRxiv - Microbiology 2020Quote: ... Goat IgG isotype control antibody was from Invitrogen (#02-6202) and Anti-VSV-G antibody from Kerafast (#EB0010). Antibodies for validation of our proteomics results and the follow-up studies included APOE (Proteintech ...
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bioRxiv - Immunology 2022Quote: ... The cells were subsequently infected with G*ΔG-VSV (Kerafast) that packages expression cassettes for firefly luciferase instead of VSV-G in the VSV genome ...
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bioRxiv - Bioengineering 2021Quote: ... osteoclast differentiation marker tartrate-resistant acid phosphatase (TRAP; Kerafast, MA, USA) and Hoechst (Sigma Aldrich Inc) ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Microbiology 2020Quote: ... cells were infected with VSV-luc (VSV-G) (Kerafast, EH1020-PM) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with VSV-G*ΔG-luciferase (Kerafast, EH1020-PM). The supernatant containing sarbecovirus pseudotyped VSV was collected 2 days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with VSV-luc (VSV-G) (Kerafast, EH1020-PM) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... and protein synthesis level was determined by Western blot using anti-puromycin antibody (KeraFAST, Cat#EQ0001, 1:1000). The combined intensity of proteins ranging from 20 to 200 kDa was quantified and normalized to the level of β-actin.
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with replication restricted rVSVΔG∗G-GFP virus (Kerafast, #EH1019-PM) at ~5 MOI for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... were inoculated with VSV-G pseudotyped ΔG-luciferase or GFP VSV (Kerafast, MA). After 2 hr at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... were inoculated with VSV-G pseudotyped ΔG-luciferase or GFP VSV (Kerafast, MA). After 2 hr at 37°C ...
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bioRxiv - Immunology 2021Quote: ... the packaging cells were infected with VSV-G*ΔG-luciferase (Kerafast, EH1020-PM). 48 hours after infection the supernatant containing Sarbecovirus pseudotyped VSV-luc was collected ...
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bioRxiv - Immunology 2023Quote: ... were inoculated with VSV-G pseudotyped ΔG-luciferase or GFP VSV (Kerafast, MA). After 2 h incubation at 37°C ...
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bioRxiv - Neuroscience 2020Quote: Detection of newly synthesized proteins by proximity ligation was performed as previously described6 ssing a mouse anti-puromycin (Kerafast, 1:500) antibody in combination with rabbit anti-beta actin (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The denatured TCA-treated proteins were subjected to immunoblot analysis for VSV M and EBOV GP protein levels using anti-VSV M (1:2500, Kerafast, EB0011) and anti-EBOV GP (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 293T/Spike cells were incubated with pseudotyped G*ΔG-GFP rVSV (Kerafast EH1024-PM) at MOI ∼3 for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was expressed in LOBSTR (Kerafast) E.coli competent cells transformed with pTf16 chaperone protein tag (TaKaRa Bio ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-Matrix Protein (Kerafast, Inc.; EMS009) or goat anti-Influenza A Virus (Abcam ...
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bioRxiv - Neuroscience 2019Quote: Detection of newly synthesized proteins by proximity ligation was carried out using anti-puromycin antibodies (mouse anti-Puromycin from Kerafast, 1:500-1000) in combination with protein-specific antibodies (rabbit anti-RapGEF4 from Invitrogen ...
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bioRxiv - Immunology 2023Quote: The pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV was purchased from Kerafast (catalog number: EH1020-PM) and was used to produce VSV-ΔG pseudotyped with the SARS-CoV-2 spike protein ...
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bioRxiv - Microbiology 2021Quote: ... while simultaneously labeling for cysteine sulfenic acids (CSAs) with 1mM biotin-1,3-cyclopentanedione (BP1) (Kerafast, Boston, MA, USA). Lysis buffer was prepared fresh before use and BP1 was added immediately prior to use ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA–RNA hybrids were immunoprecipitated from total nucleic acids using mouse anti-DNA–RNA hybrid S9.6 (Kerafast, ENH001) DRIP binding buffer and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and cells were washed with PBS before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralise residual input virus ...
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bioRxiv - Microbiology 2023Quote: Recombinant Vesicular Stomatitis Virus (VSV) stocks harboring a Firefly Luciferase gene instead of VSV G (Kerafast, EH1020-PM) were pseudotyped with either the VSV G protein (VSV-G ...
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bioRxiv - Molecular Biology 2021Quote: ... eEF3 protein was detected with anti-eEF3 antibody (Kerafast ED7003) and Glucose-6-phosphate dehydrogenase (G-6-PDH ...
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bioRxiv - Microbiology 2020Quote: ... rat anti-MLV transmembrane protein/p15E (clone 42/114, Kerafast), rabbit anti-myc (Cell Signaling Technology) ...
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bioRxiv - Microbiology 2019Quote: ... IAV matrix protein monoclonal antibody (E10; catalog no. EMS009, Kerafast) at 1:1000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Cells were subsequently washed five times and supplied with fresh DMEM + 10% FBS medium + anti-VSV-G antibody (Kerafast). Cell-free supernatants were harvested at 24 h after transduction ...
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bioRxiv - Microbiology 2022Quote: ... and for recombinant protein expression E .coli Lobstr cells (Kerafast EC1002) were used respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were infected at a multiplicity of infection of 3-5 for 2 hours at 37°C with rVSV-ΔG pseudovirus complemented in trans with the G glycoprotein and bearing a luciferase gene (Kerafast). Cells were then washed 6 times with DMEM + 10% FBS and returned to culture ...
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bioRxiv - Microbiology 2020Quote: ... Cells were rinsed with PBS one hour after infection and replace with complete media supplemented with 2 microliters of mouse hybridoma supernatant containing anti-VSV-G antibody I1 (Kerafast) to neutralize input virus ...
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bioRxiv - Immunology 2020Quote: ... after which medium was removed and cells were washed with PBS before complete medium supplemented with anti-VSV-G antibody (8G5F11, Kerafast) was added to the cells ...
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bioRxiv - Microbiology 2023Quote: ... a BlueScript backbone plasmid under T7 polymerase promoter control encoding VSV Indiana backbone in which the VSV glycoprotein (G) gene has been replaced with GFP (pVSV-ΔG-GFP) was purchased from Kerafast. The pVSV-ΔG-GFP was modified by cloning the codon optimized ORF of RABV G protein of Egyptian strain (GenBank accession number MK760770.1 ...
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bioRxiv - Immunology 2020Quote: ... the inoculum was removed and cells were washed with PBS before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralise residual input virus ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with phosphate buffered saline (PBS) before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralize residual VSV-G-complemented input virus ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with phosphate buffered saline (PBS) before medium supplemented with anti-VSV-G antibody (clone 8G5F11, Kerafast Inc.) was added to neutralize residual VSV-G-complemented input virus ...
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bioRxiv - Microbiology 2021Quote: ... Pseudoviruses were generated in BHK-21/WI-2 cells using a pseudotyped ΔG-GFP (G*ΔG-GFP) rVSV (Kerafast, Boston, MA, USA) in addition to spike sequences cloned into the paT7- Spike plasmid as described previously (21) ...
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bioRxiv - Microbiology 2022Quote: ... serum-containing medium was replaced by serum-free DMEM and cells were inoculated with MOI 3 of VSVΔG*/Luc-G (Kerafast, Boston, MA, USA) for the production of luciferase-expressing pseudotypes ...
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bioRxiv - Immunology 2021Quote: ... we generated a replication defective Vesicular Stomatitis Virus (VSV) PV in which the backbone was provided by a pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV (Kerafast, Boston, MA, USA), that packages the expression cassette for firefly luciferase instead of VSV-G in the VSV genome ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was evaluated as FFU/mL using the anti-VSV-Matrix protein 23H12 antibody (Kerafast Inc.) to detect infected cells.
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bioRxiv - Microbiology 2021Quote: ... VSV M protein was detected using anti-VSV M antibody clone [23H12] (KeraFast, Boston, MA, Cat.# EB0011) at 1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-puromycin (IB, 1:1000, IF 1:3500, Kerafast,), guinea pig anti-MAP2 (IF 1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... while the lower portion of the membrane was probed with anti-VSV matrix protein antibody (Kerafast, EB0011; loading control) diluted 1:2,500 in 5% skim milk solution ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses bearing mutant S proteins were produced using the ΔG luciferase recombinant vesicular stomatitis virus (rVSV) system (KeraFast EH1025-PM)(Whitt ...
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bioRxiv - Microbiology 2022Quote: ... 34850, 1:2,000 dilution) (rabbit anti-ORF8, GeneTex, Cat #: GTX135591, 1:1,000 dilution) (mouse anti-VSV-M, Kerafast, Cat #: EB0011, 1:100,000 dilution), prepared in WB blocking buffer (5% skim milk (Bio-Rad ...