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Citations for KeraFAST :
1 - 50 of 133 citations for 8 Chloro 4 methyl 1 2 dihydroquinolin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Immunology 2021Quote: ... 2021) and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Microbiology 2021Quote: ... or BHK-21/WI-2 (Kerafast) cells were transfected with SARS-CoV-2 Spike expression plasmid (pTT5 SARS-CoV-2 SΔ21) ...
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bioRxiv - Microbiology 2022Quote: ... BHK21/WI-2 cells (Kerafast #EH1011) were maintained in DMEM (Thermo Fisher #11965092 ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S-expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described.6 For the neutralization assay ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S expression plasmid and subsequently infected with VSV△G-firefly-luciferase as previously described [40] ...
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bioRxiv - Immunology 2021Quote: ... BHK21/WI-2 cells (Kerafast, EH1011) were transfected with the Wuhan-1 strain (Genbank # ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) were maintained in DMEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast ...
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bioRxiv - Immunology 2021Quote: ... BHK-21/WI-2 cells (Kerafast, EH1011) were transfected with the spike expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described (Whitt ...
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bioRxiv - Biochemistry 2023Quote: ... 20% glycerol] and 2 µL of Marathon (Kerafast) were added to the solution ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
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bioRxiv - Immunology 2022Quote: VSV pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously.66 BHK-21/WI-2 cells were plated into a 6 well plate ...
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bioRxiv - Immunology 2022Quote: VSV vectors pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously [51] ...
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bioRxiv - Microbiology 2020Quote: ... and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene ...
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bioRxiv - Immunology 2021Quote: Baby Hamster kidney BHK-21/WI-2 cell line (Kerafast, EH1011), African Green monkey kidney-derived Vero E6 cell line (ATCC ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were gently washed three times 2-hour post-infection and incubated in DMEM containing 1:1000 dilution of anti-VSV-G antibody (Kerafast: 8G5F11) to eliminate residual ΔG-VSV particles at 37°C ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were subsequently briefly rinsed thrice with 2% BSA/0.05% Tween (in PBS) and incubated with mouse anti-DNA– RNA hybrid S9.6 (Kerafast, ENH001; 1:100) and rabbit anti-nucleolin (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells were purchased from Kerafast (Boston, MA, USA) and cultured in D5+ media ...
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bioRxiv - Microbiology 2022Quote: Baby hamster kidney cells (BHK-21/WI-2, Kerafast # EH1011, Boston, MA, USA) and African Green Monkey Cell Line (VeroE6 ...
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bioRxiv - Microbiology 2020Quote: ... and were infected 2 days post-transfection with >107 infectious units/well of VSVΔG-GFP (Kerafast, (22)) ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the following primary antibodies overnight at 4°C for S9.6-HIV-1-IN_PLA: mouse anti-DNA–RNA hybrid S9.6 (1:250; Kerafast, ENH001) and rabbit anti-GFP (1:500 ...
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bioRxiv - Immunology 2020Quote: ... HEK293T/17 monolayers transfected to express SARS-CoV-2 S truncated of the C-terminal cytoplasmic 19 amino acids (SARS-CoV-2-S-CΔ19) were inoculated with VSVΔG-GFP vector (rescued from pVSVΔG-GFP plasmid expression vector; Kerafast Inc.). After incubation for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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bioRxiv - Microbiology 2020Quote: ... Cells were rinsed with PBS one hour after infection and replace with complete media supplemented with 2 microliters of mouse hybridoma supernatant containing anti-VSV-G antibody I1 (Kerafast) to neutralize input virus ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated O/N at 4°C with a monoclonal S9.6 antibody (Kerafast, 1:100) diluted in 3%PBS-BSA ...
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bioRxiv - Molecular Biology 2023Quote: Reporter gene Renilla luciferase was cloned into the multiple cloning site MCS-2 of plasmid pVSV-ΔG-N/P-MCS2-2.6 (Kerafast, Boston Massachusetts), which encodes the positive-sense RNA of a replication-defective recombinant vesicular stomatitis virus (rVSV ...
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bioRxiv - Microbiology 2021Quote: ... Pseudoviruses were generated in BHK-21/WI-2 cells using a pseudotyped ΔG-GFP (G*ΔG-GFP) rVSV (Kerafast, Boston, MA, USA) in addition to spike sequences cloned into the paT7- Spike plasmid as described previously (21) ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight on a rotating wheel at 4 °C in presence of 1 μg of S9.6 antibody (Kerafast) and 20 μl of protein G sepharose beads (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... at a density of one cell/per well with pre-coated EL4-B5 feeder cells (Kerafast). The feeder cells were irradiated with 50 Gy in a gamma radiation chamber before use ...
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bioRxiv - Pathology 2019Quote: R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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bioRxiv - Cell Biology 2024Quote: ... we utilized a human immortalized (HuIm) RPTEC line (Clone TH1, passage 8, Cat No. ECH001, Kerafast, Boston, MA), as control ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2022Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37 °C with 8% CO2 and VSV-G pseudo-typed DG-luciferase (G*DG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1xDPBS three times ...
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bioRxiv - Neuroscience 2022Quote: ... We incubated 48h at 4°C with rat anti-tdTomato (16D7, Kerafast) diluted 1:500 in a solution of 3% bovine albumin serum and 0.3% Triton X-100 prepared in PBS 1x ...
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bioRxiv - Immunology 2022Quote: ... Membranes were probed overnight at 4°C with anti-RNA:DNA hybrid (S9.6) (Kerafast) at 1:10,000 dilution or dsDNA antibody (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... (4-(1,2,4,5-tetrzain-3-yl)phenyl)methanamine (tetrazine amine) was purchased from Kerafast (FCC659, lot: 2014). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene amine ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated overnight at 4°C with anti-M2 monoclonal antibody (E10; catalog no. EMS009, Kerafast). Three washes were performed prior to addition of goat anti-mouse Alexa Fluor-647 secondary antibody (catalog no ...
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bioRxiv - Genomics 2022Quote: ... 275 mM NaCl) and incubated overnight at 4°C in the presence of 1.5 µg of S9.6 purified antibody (Kerafast). Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA/DNA hybrids were immunoprecipitated from digested DNA (4 μg) using the S9.6 (10 μg) antibody (Kerafast EHN001), or mouse IgG antibody as negative control ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-puromycin (IB, 1:1000, IF 1:3500, Kerafast,), guinea pig anti-MAP2 (IF 1:1000 ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Microbiology 2022Quote: ... 34850, 1:2,000 dilution) (rabbit anti-ORF8, GeneTex, Cat #: GTX135591, 1:1,000 dilution) (mouse anti-VSV-M, Kerafast, Cat #: EB0011, 1:100,000 dilution), prepared in WB blocking buffer (5% skim milk (Bio-Rad ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:3500 mouse Puromycin (Kerafast), 1:1000 rabbit β-Tubulin 9F3 (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... homopropargyl glycine (1 mM; Kerafast), and appropriate drug for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... respectively (diluted 1:50, Kerafast) followed by Alexa Fluor 594 anti-rat IgG (diluted 1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-tdTomato (1:500, Kerafast EST203). Anti-cathepsin L antibody (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Toxoplasma α-Centrin-1 (Kerafast company) 1:2,000 ...