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Citations for KeraFAST :
1 - 50 of 139 citations for 6 Chloropyrazolo 1 5 a pyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Modular structure of RNA 3’ processing condensates involving the Arabidopsis RNA binding protein FCAbioRxiv - Molecular Biology 2024Quote: ... 5 μg of S9.6 antibody (1:100 dilution, ENH001, Kerafast) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked with TBST-5% non-fat milk at room temperature for 1 h and incubated with 1:1000 s9.6 (Kerafast, ENH001) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... C57BL/6 wild type and iNOS knockout mutant (Kerafast) cell lines were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Bioengineering 2021Quote: ... osteoclast differentiation marker tartrate-resistant acid phosphatase (TRAP; Kerafast, MA, USA) and Hoechst (Sigma Aldrich Inc) ...
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bioRxiv - Neuroscience 2020Quote: ... hiPSCs were seeded onto freshly coated 6-well plates 3 days prior to transduction with premade LV-CAG-eGFP lentivirus (Kerafast FCT149, 1 × 108 CFU/ml). Polybrene (2 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg chromatin was used for immunoprecipitation (IP) reactions in 100 µL aliquots with 5 µg S9.6 antibody (Kerafast), as per the kit protocol ...
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bioRxiv - Cell Biology 2022Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA-RNA hybrid [S9.6] antibody (Kerafast, 1:1000) overnight at 4°C ...
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bioRxiv - Pathology 2019Quote: R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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bioRxiv - Microbiology 2021Quote: Murine C57BL/6 MC-38 colorectal tumor cells were obtained from Kerafast (ENH204-FP) and maintained in 5% CO2 at 37°C in Dulbecco’s modified MEM (DMEM ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were gently washed three times 2-hour post-infection and incubated in DMEM containing 1:1000 dilution of anti-VSV-G antibody (Kerafast: 8G5F11) to eliminate residual ΔG-VSV particles at 37°C ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The slides were subsequently briefly rinsed thrice with 2% BSA/0.05% Tween (in PBS) and incubated with mouse anti-DNA– RNA hybrid S9.6 (Kerafast, ENH001; 1:100) and rabbit anti-nucleolin (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... or BHK-21/WI-2 (Kerafast) cells were transfected with SARS-CoV-2 Spike expression plasmid (pTT5 SARS-CoV-2 SΔ21) ...
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bioRxiv - Microbiology 2022Quote: ... BHK21/WI-2 cells (Kerafast #EH1011) were maintained in DMEM (Thermo Fisher #11965092 ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S-expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described.6 For the neutralization assay ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S expression plasmid and subsequently infected with VSV△G-firefly-luciferase as previously described [40] ...
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bioRxiv - Immunology 2021Quote: ... BHK21/WI-2 cells (Kerafast, EH1011) were transfected with the Wuhan-1 strain (Genbank # ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) were maintained in DMEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast ...
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bioRxiv - Immunology 2021Quote: ... BHK-21/WI-2 cells (Kerafast, EH1011) were transfected with the spike expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described (Whitt ...
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bioRxiv - Microbiology 2021Quote: ... while simultaneously labeling for cysteine sulfenic acids (CSAs) with 1mM biotin-1,3-cyclopentanedione (BP1) (Kerafast, Boston, MA, USA). Lysis buffer was prepared fresh before use and BP1 was added immediately prior to use ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA–RNA hybrids were immunoprecipitated from total nucleic acids using mouse anti-DNA–RNA hybrid S9.6 (Kerafast, ENH001) DRIP binding buffer and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... biotinylated medium chain polyP (5-20 ug/uL, Kerafast) or 5’-biotinylated single-strand DNA (5 ug/uL ...
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bioRxiv - Cell Biology 2021Quote: ... were immunoprecipitated using 5 μg of S9.6 antibody (Kerafast) and 20 μl of Protein G Dynabeads (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... 20% glycerol] and 2 µL of Marathon (Kerafast) were added to the solution ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
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bioRxiv - Microbiology 2021Quote: ... Samples were loaded onto either 6% or 10% TBE-Urea Gel (Thermo Fischer Scientific) along with a biotinylated sRNA ladder (Kerafast). Gels were run in 1x TBE buffer at 180V for 60 min (6% gels ...
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bioRxiv - Microbiology 2021Quote: RNA samples were loaded onto either 6% or 10% TBE-Urea Gel (Thermo Fischer Scientific) with a biotinylated RNA ladder (Kerafast). Gels were run in 1x TBE buffer at 180V for 60 minutes (6% gels ...
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bioRxiv - Immunology 2022Quote: VSV pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously.66 BHK-21/WI-2 cells were plated into a 6 well plate ...
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bioRxiv - Immunology 2022Quote: VSV vectors pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously [51] ...
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bioRxiv - Microbiology 2020Quote: ... and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene ...
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bioRxiv - Immunology 2021Quote: Baby Hamster kidney BHK-21/WI-2 cell line (Kerafast, EH1011), African Green monkey kidney-derived Vero E6 cell line (ATCC ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells were purchased from Kerafast (Boston, MA, USA) and cultured in D5+ media ...
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bioRxiv - Microbiology 2022Quote: Baby hamster kidney cells (BHK-21/WI-2, Kerafast # EH1011, Boston, MA, USA) and African Green Monkey Cell Line (VeroE6 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-puromycin (IB, 1:1000, IF 1:3500, Kerafast,), guinea pig anti-MAP2 (IF 1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA–RNA hybrid [S9.6] antibody (Kerafast) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.0/140 mM NaCl/0.05% Triton X-100) and 5 μg of the S9.6 antibody (Kerafast, 1mg/ml) or 5 μg of the BrdU antibody (#555627 ...
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bioRxiv - Microbiology 2021Quote: ... The blocking solution was removed and replaced with fresh TBST containing 5% (w/v) milk and S9.6 anti-DNA-RNA Hybrid Antibody (Kerafast) at 1μg/ml and incubated at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... and were infected 2 days post-transfection with >107 infectious units/well of VSVΔG-GFP (Kerafast, (22)) ...
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bioRxiv - Immunology 2021Quote: ... 2021) and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Microbiology 2020Quote: ... cells were infected at a multiplicity of infection of 3-5 with rVSV-ΔG pseudovirus bearing a luciferase gene (Kerafast) for 2 hours at 37°C and then washed 6 times with 1X PBS ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was generated by nucleofection of BHK cells (maintained in DMEM/F12 with 10%FBS and 5%TPB) with Spike-expressing plasmid followed by transduction with G-pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV (Kerafast) 18-24 hours later ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2020Quote: ... HEK293T/17 monolayers transfected to express SARS-CoV-2 S truncated of the C-terminal cytoplasmic 19 amino acids (SARS-CoV-2-S-CΔ19) were inoculated with VSVΔG-GFP vector (rescued from pVSVΔG-GFP plasmid expression vector; Kerafast Inc.). After incubation for 1 h at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...