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Citations for KeraFAST :
1 - 50 of 119 citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... (4-(1,2,4,5-tetrzain-3-yl)phenyl)methanamine (tetrazine amine) was purchased from Kerafast (FCC659, lot: 2014). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene amine ...
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bioRxiv - Microbiology 2019Quote: ... using the hybrid-targeting S9.6 (4.5 ng, Kerafast) and α-γH2A (10 μl ...
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bioRxiv - Cell Biology 2024Quote: ... we utilized a human immortalized (HuIm) RPTEC line (Clone TH1, passage 8, Cat No. ECH001, Kerafast, Boston, MA), as control ...
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bioRxiv - Cell Biology 2021Quote: ... R-Loops were immunoprecipitated using 4.5 ng of S9.6 antibody (Kerafast). RNase H1 treated samples were prepared as described52 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2022Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37 °C with 8% CO2 and VSV-G pseudo-typed DG-luciferase (G*DG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1xDPBS three times ...
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bioRxiv - Neuroscience 2020Quote: ... hiPSCs were seeded onto freshly coated 6-well plates 3 days prior to transduction with premade LV-CAG-eGFP lentivirus (Kerafast FCT149, 1 × 108 CFU/ml). Polybrene (2 μg/ml ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the following primary antibodies overnight at 4°C for S9.6-HIV-1-IN_PLA: mouse anti-DNA–RNA hybrid S9.6 (1:250; Kerafast, ENH001) and rabbit anti-GFP (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated O/N at 4°C with a monoclonal S9.6 antibody (Kerafast, 1:100) diluted in 3%PBS-BSA ...
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bioRxiv - Microbiology 2023Quote: ... C57BL/6 wild type and iNOS knockout mutant (Kerafast) cell lines were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s medium (DMEM ...
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bioRxiv - Bioengineering 2021Quote: ... osteoclast differentiation marker tartrate-resistant acid phosphatase (TRAP; Kerafast, MA, USA) and Hoechst (Sigma Aldrich Inc) ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight on a rotating wheel at 4 °C in presence of 1 μg of S9.6 antibody (Kerafast) and 20 μl of protein G sepharose beads (GE Healthcare ...
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bioRxiv - Pathology 2019Quote: R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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bioRxiv - Microbiology 2021Quote: Murine C57BL/6 MC-38 colorectal tumor cells were obtained from Kerafast (ENH204-FP) and maintained in 5% CO2 at 37°C in Dulbecco’s modified MEM (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... while simultaneously labeling for cysteine sulfenic acids (CSAs) with 1mM biotin-1,3-cyclopentanedione (BP1) (Kerafast, Boston, MA, USA). Lysis buffer was prepared fresh before use and BP1 was added immediately prior to use ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA–RNA hybrids were immunoprecipitated from total nucleic acids using mouse anti-DNA–RNA hybrid S9.6 (Kerafast, ENH001) DRIP binding buffer and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... We incubated 48h at 4°C with rat anti-tdTomato (16D7, Kerafast) diluted 1:500 in a solution of 3% bovine albumin serum and 0.3% Triton X-100 prepared in PBS 1x ...
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bioRxiv - Microbiology 2021Quote: ... Samples were loaded onto either 6% or 10% TBE-Urea Gel (Thermo Fischer Scientific) along with a biotinylated sRNA ladder (Kerafast). Gels were run in 1x TBE buffer at 180V for 60 min (6% gels ...
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bioRxiv - Microbiology 2021Quote: RNA samples were loaded onto either 6% or 10% TBE-Urea Gel (Thermo Fischer Scientific) with a biotinylated RNA ladder (Kerafast). Gels were run in 1x TBE buffer at 180V for 60 minutes (6% gels ...
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bioRxiv - Immunology 2022Quote: ... Membranes were probed overnight at 4°C with anti-RNA:DNA hybrid (S9.6) (Kerafast) at 1:10,000 dilution or dsDNA antibody (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated overnight at 4°C with anti-M2 monoclonal antibody (E10; catalog no. EMS009, Kerafast). Three washes were performed prior to addition of goat anti-mouse Alexa Fluor-647 secondary antibody (catalog no ...
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bioRxiv - Microbiology 2020Quote: ... cells were infected at a multiplicity of infection of 3-5 with rVSV-ΔG pseudovirus bearing a luciferase gene (Kerafast) for 2 hours at 37°C and then washed 6 times with 1X PBS ...
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bioRxiv - Genomics 2022Quote: ... 275 mM NaCl) and incubated overnight at 4°C in the presence of 1.5 µg of S9.6 purified antibody (Kerafast). Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA/DNA hybrids were immunoprecipitated from digested DNA (4 μg) using the S9.6 (10 μg) antibody (Kerafast EHN001), or mouse IgG antibody as negative control ...
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bioRxiv - Plant Biology 2023Quote: ... Sections were treated with blocking solution containing 3% (w/v) skim milk in PBS for 30 min and then with LM5 antibody (Kerafast, USA) diluted 5-fold in blocking solution for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... serum-containing medium was replaced by serum-free DMEM and cells were inoculated with MOI 3 of VSVΔG*/Luc-G (Kerafast, Boston, MA, USA) for the production of luciferase-expressing pseudotypes ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-puromycin (IB, 1:1000, IF 1:3500, Kerafast,), guinea pig anti-MAP2 (IF 1:1000 ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Microbiology 2022Quote: ... 34850, 1:2,000 dilution) (rabbit anti-ORF8, GeneTex, Cat #: GTX135591, 1:1,000 dilution) (mouse anti-VSV-M, Kerafast, Cat #: EB0011, 1:100,000 dilution), prepared in WB blocking buffer (5% skim milk (Bio-Rad ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:3500 mouse Puromycin (Kerafast), 1:1000 rabbit β-Tubulin 9F3 (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... homopropargyl glycine (1 mM; Kerafast), and appropriate drug for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... respectively (diluted 1:50, Kerafast) followed by Alexa Fluor 594 anti-rat IgG (diluted 1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-tdTomato (1:500, Kerafast EST203). Anti-cathepsin L antibody (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Toxoplasma α-Centrin-1 (Kerafast company) 1:2,000 ...
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bioRxiv - Plant Biology 2021Quote: ... anti-ARP6 (1:200, Kerafast, EGA929), anti-DET3 (1:10000 ...
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bioRxiv - Biochemistry 2022Quote: ... α-puromycin (dilution 1:10,000; Kerafast EQ0001), and α-β-actin (dilution 1:30,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-puromycin (1:2000, Kerafast, 3RH11), anti-Rpl11 (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... tdTomato [16D7] (#EST203, 1:200, Kerafast), RFP (#600-401-379 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-Puromycin (Kerafast, 1:500-1000), guinea pig anti-VGLUT1 (Synaptic Systems ...
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bioRxiv - Neuroscience 2021Quote: ... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse anti-PMY (Kerafast, 1:1000) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-mCherry antibody (1:500, Kerafast) (immunohistochemistry) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-mCherry (1:500, Kerafast, EMU106); rat anti-TFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-TFP (1:500, Kerafast, EMU104), guinea pig anti-TagBFP (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... and VSV-M (1:2500, Kerafast, EB0011).
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bioRxiv - Developmental Biology 2019Quote: ... anti-COL1A1 (LF-68, Kerafast; 1:100), AF568-conjugated goat-anti-rabbit IgG (A11011 ...
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bioRxiv - Developmental Biology 2021Quote: ... LM19 and LM20 (Kerafast, diluted 1:10) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-puromycin (1:2000 dilution, Kerafast, 3RH11), anti-Rpl11 (1:300 dilution ...
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bioRxiv - Genetics 2022Quote: ... SPP1 at 1:300 (ENH094-FP, Kerafast), SP7/OSX at 1:100 (Ab22552 ...