Labshake search
Citations for KeraFAST :
1 - 50 of 52 citations for 5 Fluoro 2 methoxy 4 pyridinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2019Quote: R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
-
bioRxiv - Microbiology 2021Quote: ... or BHK-21/WI-2 (Kerafast) cells were transfected with SARS-CoV-2 Spike expression plasmid (pTT5 SARS-CoV-2 SΔ21) ...
-
bioRxiv - Microbiology 2022Quote: ... BHK21/WI-2 cells (Kerafast #EH1011) were maintained in DMEM (Thermo Fisher #11965092 ...
-
bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S-expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described.6 For the neutralization assay ...
-
bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S expression plasmid and subsequently infected with VSV△G-firefly-luciferase as previously described [40] ...
-
bioRxiv - Immunology 2021Quote: ... BHK21/WI-2 cells (Kerafast, EH1011) were transfected with the Wuhan-1 strain (Genbank # ...
-
bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) were maintained in DMEM (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast ...
-
bioRxiv - Immunology 2021Quote: ... BHK-21/WI-2 cells (Kerafast, EH1011) were transfected with the spike expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described (Whitt ...
-
bioRxiv - Biochemistry 2019Quote: ... biotinylated medium chain polyP (5-20 ug/uL, Kerafast) or 5’-biotinylated single-strand DNA (5 ug/uL ...
-
bioRxiv - Cell Biology 2021Quote: ... were immunoprecipitated using 5 μg of S9.6 antibody (Kerafast) and 20 μl of Protein G Dynabeads (Thermo Fisher ...
-
bioRxiv - Biochemistry 2023Quote: ... 20% glycerol] and 2 µL of Marathon (Kerafast) were added to the solution ...
-
Modular structure of RNA 3’ processing condensates involving the Arabidopsis RNA binding protein FCAbioRxiv - Molecular Biology 2024Quote: ... 5 μg of S9.6 antibody (1:100 dilution, ENH001, Kerafast) was added ...
-
bioRxiv - Neuroscience 2022Quote: ... We incubated 48h at 4°C with rat anti-tdTomato (16D7, Kerafast) diluted 1:500 in a solution of 3% bovine albumin serum and 0.3% Triton X-100 prepared in PBS 1x ...
-
bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
-
bioRxiv - Immunology 2022Quote: VSV pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously.66 BHK-21/WI-2 cells were plated into a 6 well plate ...
-
bioRxiv - Immunology 2022Quote: VSV vectors pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously [51] ...
-
bioRxiv - Immunology 2022Quote: ... Membranes were probed overnight at 4°C with anti-RNA:DNA hybrid (S9.6) (Kerafast) at 1:10,000 dilution or dsDNA antibody (Abcam ...
-
bioRxiv - Microbiology 2020Quote: ... and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene ...
-
bioRxiv - Immunology 2021Quote: Baby Hamster kidney BHK-21/WI-2 cell line (Kerafast, EH1011), African Green monkey kidney-derived Vero E6 cell line (ATCC ...
-
bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells were purchased from Kerafast (Boston, MA, USA) and cultured in D5+ media ...
-
bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated O/N at 4°C with a monoclonal S9.6 antibody (Kerafast, 1:100) diluted in 3%PBS-BSA ...
-
bioRxiv - Bioengineering 2022Quote: ... (4-(1,2,4,5-tetrzain-3-yl)phenyl)methanamine (tetrazine amine) was purchased from Kerafast (FCC659, lot: 2014). 1-bicyclo[2.2.1]hept-5-en-2-ylmethanamine (norbornene amine ...
-
bioRxiv - Microbiology 2022Quote: Baby hamster kidney cells (BHK-21/WI-2, Kerafast # EH1011, Boston, MA, USA) and African Green Monkey Cell Line (VeroE6 ...
-
bioRxiv - Microbiology 2019Quote: ... cells were incubated overnight at 4°C with anti-M2 monoclonal antibody (E10; catalog no. EMS009, Kerafast). Three washes were performed prior to addition of goat anti-mouse Alexa Fluor-647 secondary antibody (catalog no ...
-
bioRxiv - Genomics 2022Quote: ... 275 mM NaCl) and incubated overnight at 4°C in the presence of 1.5 µg of S9.6 purified antibody (Kerafast). Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare) ...
-
bioRxiv - Developmental Biology 2023Quote: ... RNA/DNA hybrids were immunoprecipitated from digested DNA (4 μg) using the S9.6 (10 μg) antibody (Kerafast EHN001), or mouse IgG antibody as negative control ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the following primary antibodies overnight at 4°C for S9.6-HIV-1-IN_PLA: mouse anti-DNA–RNA hybrid S9.6 (1:250; Kerafast, ENH001) and rabbit anti-GFP (1:500 ...
-
bioRxiv - Genetics 2019Quote: ... and incubated overnight on a rotating wheel at 4 °C in presence of 1 μg of S9.6 antibody (Kerafast) and 20 μl of protein G sepharose beads (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA–RNA hybrid [S9.6] antibody (Kerafast) overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 7.0/140 mM NaCl/0.05% Triton X-100) and 5 μg of the S9.6 antibody (Kerafast, 1mg/ml) or 5 μg of the BrdU antibody (#555627 ...
-
bioRxiv - Microbiology 2021Quote: ... The blocking solution was removed and replaced with fresh TBST containing 5% (w/v) milk and S9.6 anti-DNA-RNA Hybrid Antibody (Kerafast) at 1μg/ml and incubated at 4°C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µg chromatin was used for immunoprecipitation (IP) reactions in 100 µL aliquots with 5 µg S9.6 antibody (Kerafast), as per the kit protocol ...
-
bioRxiv - Microbiology 2020Quote: ... and were infected 2 days post-transfection with >107 infectious units/well of VSVΔG-GFP (Kerafast, (22)) ...
-
bioRxiv - Immunology 2021Quote: ... 2021) and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
-
bioRxiv - Microbiology 2020Quote: ... cells were infected at a multiplicity of infection of 3-5 with rVSV-ΔG pseudovirus bearing a luciferase gene (Kerafast) for 2 hours at 37°C and then washed 6 times with 1X PBS ...
-
bioRxiv - Immunology 2022Quote: SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was generated by nucleofection of BHK cells (maintained in DMEM/F12 with 10%FBS and 5%TPB) with Spike-expressing plasmid followed by transduction with G-pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV (Kerafast) 18-24 hours later ...
-
bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
-
bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
-
bioRxiv - Cell Biology 2022Quote: ... blocked in 5% non-fat dry milk and incubated with the anti-DNA-RNA hybrid [S9.6] antibody (Kerafast, 1:1000) overnight at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
-
bioRxiv - Cancer Biology 2023Quote: ... blocked with TBST-5% non-fat milk at room temperature for 1 h and incubated with 1:1000 s9.6 (Kerafast, ENH001) overnight at 4 °C ...
-
bioRxiv - Immunology 2020Quote: ... HEK293T/17 monolayers transfected to express SARS-CoV-2 S truncated of the C-terminal cytoplasmic 19 amino acids (SARS-CoV-2-S-CΔ19) were inoculated with VSVΔG-GFP vector (rescued from pVSVΔG-GFP plasmid expression vector; Kerafast Inc.). After incubation for 1 h at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
-
bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were rinsed with PBS one hour after infection and replace with complete media supplemented with 2 microliters of mouse hybridoma supernatant containing anti-VSV-G antibody I1 (Kerafast) to neutralize input virus ...
-
bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were gently washed three times 2-hour post-infection and incubated in DMEM containing 1:1000 dilution of anti-VSV-G antibody (Kerafast: 8G5F11) to eliminate residual ΔG-VSV particles at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: Reporter gene Renilla luciferase was cloned into the multiple cloning site MCS-2 of plasmid pVSV-ΔG-N/P-MCS2-2.6 (Kerafast, Boston Massachusetts), which encodes the positive-sense RNA of a replication-defective recombinant vesicular stomatitis virus (rVSV ...