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Citations for KeraFAST :
51 - 100 of 149 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human FN III 12,13 N-GST (“HBDII”, which is derived from heparin-binding domain II of FN; #EUR120, Kerafast). Sheared DNA molecules were then added dropwise with continuous stirring at 400∼500 rpm with the final concentration of 10 µg/ml ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated overnight at 4°C with anti-M2 monoclonal antibody (E10; catalog no. EMS009, Kerafast). Three washes were performed prior to addition of goat anti-mouse Alexa Fluor-647 secondary antibody (catalog no ...
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bioRxiv - Genomics 2022Quote: ... 275 mM NaCl) and incubated overnight at 4°C in the presence of 1.5 µg of S9.6 purified antibody (Kerafast). Antibody-associated DNA:RNA hybrids were then captured on protein G Sepharose beads (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were infected at a multiplicity of infection of 3-5 for 2 hours at 37°C with rVSV-ΔG pseudovirus complemented in trans with the G glycoprotein and bearing a luciferase gene (Kerafast). Cells were then washed 6 times with DMEM + 10% FBS and returned to culture ...
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bioRxiv - Microbiology 2022Quote: ... Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37 °C with 8% CO2 and VSV-G pseudo-typed DG-luciferase (G*DG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1xDPBS three times ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2024Quote: ... Cells were cultured overnight at 37°C with 5% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase, Kerafast) was used to infect the cells in DMEM at a multiplicity of infection of 5 for 4 h before washing the cells with 1×DPBS three times ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was evaluated as FFU/mL using the anti-VSV-Matrix protein 23H12 antibody (Kerafast Inc.) to detect infected cells.
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bioRxiv - Microbiology 2021Quote: ... VSV M protein was detected using anti-VSV M antibody clone [23H12] (KeraFast, Boston, MA, Cat.# EB0011) at 1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-puromycin (IB, 1:1000, IF 1:3500, Kerafast,), guinea pig anti-MAP2 (IF 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... while the lower portion of the membrane was probed with anti-VSV matrix protein antibody (Kerafast, EB0011; loading control) diluted 1:2,500 in 5% skim milk solution ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate wild-type NEC220 17,28 ...
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bioRxiv - Microbiology 2022Quote: ... 34850, 1:2,000 dilution) (rabbit anti-ORF8, GeneTex, Cat #: GTX135591, 1:1,000 dilution) (mouse anti-VSV-M, Kerafast, Cat #: EB0011, 1:100,000 dilution), prepared in WB blocking buffer (5% skim milk (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: In vivo muscle protein synthesis was assessed using the non-isotopic SUnSET method and an antibody against puromycin (Kerafast, Boston, MA), as described previously [31 ...
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bioRxiv - Physiology 2020Quote: ... Protein synthesis was determined in the freely fed state using the SUnSET method and an antibody against puromycin (Kerafast, Boston, MA)25,26 ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:3500 mouse Puromycin (Kerafast), 1:1000 rabbit β-Tubulin 9F3 (Cell Signaling Technology) ...
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bioRxiv - Biochemistry 2022Quote: ... homopropargyl glycine (1 mM; Kerafast), and appropriate drug for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... respectively (diluted 1:50, Kerafast) followed by Alexa Fluor 594 anti-rat IgG (diluted 1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-tdTomato (1:500, Kerafast EST203). Anti-cathepsin L antibody (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Toxoplasma α-Centrin-1 (Kerafast company) 1:2,000 ...
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bioRxiv - Plant Biology 2021Quote: ... anti-ARP6 (1:200, Kerafast, EGA929), anti-DET3 (1:10000 ...
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bioRxiv - Biochemistry 2022Quote: ... α-puromycin (dilution 1:10,000; Kerafast EQ0001), and α-β-actin (dilution 1:30,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-puromycin (1:2000, Kerafast, 3RH11), anti-Rpl11 (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... tdTomato [16D7] (#EST203, 1:200, Kerafast), RFP (#600-401-379 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-Puromycin (Kerafast, 1:500-1000), guinea pig anti-VGLUT1 (Synaptic Systems ...
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bioRxiv - Neuroscience 2021Quote: ... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse anti-PMY (Kerafast, 1:1000) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-mCherry antibody (1:500, Kerafast) (immunohistochemistry) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-mCherry (1:500, Kerafast, EMU106); rat anti-TFP (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-TFP (1:500, Kerafast, EMU104), guinea pig anti-TagBFP (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... and VSV-M (1:2500, Kerafast, EB0011).
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bioRxiv - Developmental Biology 2019Quote: ... anti-COL1A1 (LF-68, Kerafast; 1:100), AF568-conjugated goat-anti-rabbit IgG (A11011 ...
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bioRxiv - Developmental Biology 2021Quote: ... LM19 and LM20 (Kerafast, diluted 1:10) overnight at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-puromycin (1:2000 dilution, Kerafast, 3RH11), anti-Rpl11 (1:300 dilution ...
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bioRxiv - Genetics 2022Quote: ... SPP1 at 1:300 (ENH094-FP, Kerafast), SP7/OSX at 1:100 (Ab22552 ...
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bioRxiv - Cell Biology 2024Quote: ... anti-S9.6 (mouse-monoclonal, Kerafast; 1:200), anti-PCNA (rabbit-polyclonal ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (Bigalke et al ...
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bioRxiv - Genomics 2019Quote: ... Antibodies used for immunostaining include the following: primary antibodies (anti-γH2A.X, Cell Signaling, 1:500; anti-FMRP, Cell signaling, 1:200; S9.6, Kerafast, 1:500 for lymphoblasts and 1:120 for fibroblasts ...
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bioRxiv - Microbiology 2021Quote: Plasmids encoding HSV-1 UL31 1-306 (pKH90) and UL34 1-220 (pJB02) were co-transformed into Escherichia coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC220 (NEC construct absent of the UL34 C-terminal transmembrane helix)14 ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked with TBST-5% non-fat milk at room temperature for 1 h and incubated with 1:1000 s9.6 (Kerafast, ENH001) overnight at 4 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and/or VSV-M (Kerafast; 23H12; 1:5,000) expression on 10% Bis-Tris PAGE gel (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... and Mouse Anti-Puromycin(PMY) (Kerafast, 1:1000) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and 1 mM biotin-1,3-cyclopentanedione (BP1; Kerafast). The samples were incubated for 2 h at 4 °C on a tube rotator ...
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bioRxiv - Microbiology 2022Quote: ... and/or VSV-M (Kerafast, 23H12, 1:5000). Signal was detected using SuperSignal West substrate (Thermo-Fisher).
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bioRxiv - Neuroscience 2020Quote: ... guinea pig anti-TagBFP (1:500, Kerafast, EMU108); Rabbit anti-RFP (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... LM19 and LM20 (1:100 dilution, Kerafast, USA) were used as the primary antibodies to detect (1,3;1,4)-β-glucan ...