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Citations for PolyScience :
1 - 50 of 106 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The recombinant vectors were transiently transfected into HEK293F cells with polyethyleneimine (Polyscience). After three days of expression ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were fixed in 2 % paraformaldehyde (Polyscience; Hirschberg an der Bergstrasse ...
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bioRxiv - Microbiology 2021Quote: ... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected using the PEImax (Cat# 24765-2, MW 40,000, Polyscience, Inc.) at a ratio of 1:3 of DNA ...
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... or pcDNA3.1-FLAG-hSF3B1-K700E and 0.5 µg of the respective minigene construct using the ratio 1:2 of DNA:PEI MAX (PolyScience, #24765-1) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-4 cell stage embryos were mounted between two cover slips in M9 buffer containing 20 μm microspheres (Polyscience). Dual-color 3D time-lapse imaging was performed using a Spinning disc confocal microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected using PEI (23966-1, Polyscience, Warrington, PA) with the pLentiCRISPRv2-LoxPv1 gRNA containing plasmid ...
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bioRxiv - Genomics 2023Quote: ... Polyethylenimine (PEI) (Polyscience 23966-2) was added at a 3:1 PEI:DNA ratio and the mixture briefly vortexed and incubated at RT for 10-20 mins ...
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bioRxiv - Genetics 2023Quote: ... 15 μg DNA was incubated in 600 μL Opti-MEM and 60 μL of 1 mg/mL linear PEI (Polyscience, 23966-2) for 15 minutes at room temperature before adding to cells dropwise ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection of HEK293T cells was carried out using 1 mg/ml Polyethyleneimine MAX (Polyscience). Mycoplasma contamination was monitored by DAPI staining.
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bioRxiv - Immunology 2021Quote: ... contrasted with 2% uranyl acetate (Polyscience, 21447) and lead citrate(Reynold ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded onto assay plates and transfected using polyethyleneimine (PEI, Polyscience, 1 mg/mL). Details on the conditions for each experiment can be found in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
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bioRxiv - Neuroscience 2022Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
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bioRxiv - Cell Biology 2021Quote: ... ∼2 × 107 3-μm latex NH2-beads (Polyscience) were activated with 8% glutaraldehyde for 4 h at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded into multi-well plates and transfected with polyethyleneimine (PEI, 1 mg/ml in H2O, pH 7, Polyscience, catalog no. 23966-1). Therefore ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then transfected with 1μg sgRNA plasmid by using 1 mg/ml Polyethyleneimine MAX (Polyscience). Puromycin was used to selecte the transfected cells ...
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bioRxiv - Developmental Biology 2024Quote: ... elegans were mounted on 2% agarose (w/v in water) dry pads immersed in 2% polystyrene bead (0.1 mm diameter, Polyscience, 00876-15) diluted in M9 buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Forty microliters of 2 mg/mL PEI “Max” (Polyscience) was diluted in 1.25 mL of OptiMEM and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Bioengineering 2022Quote: ... then Omicron spike protein expression vectors were combined with HIV-1 structural corresponding plasmids and co-transfected into HEK-293T cells with PEI (1mg/ml, PEI MAX, Polyscience). Two-day post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Immunology 2023Quote: ... and all plasmid constructs used throughout the study were transfected into cells using PEI transfection reagent (Polyscience, Cat; 24765-1) at a concentration of 1□µg/ml ...
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bioRxiv - Immunology 2021Quote: ... pαH-MERS RBD-Fc using 2 mg of polyethyleneimine (Polyscience, Cat# 24765). After 6 days of growth ...
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bioRxiv - Molecular Biology 2023Quote: ... Rev and Gag-pol using PEI reagent (PEI MAX, Polyscience, 24765-2). The supernatant was collected after 48 and 72 h ...
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bioRxiv - Immunology 2022Quote: The heavy and light chain plasmids were then co-transfected into HEK293 cells us-ing PEI MAX® (Cat: 24765-1, PolyScience). The production supernatant was harvested af-ter 14 days post-transfection and purified using AKTA equipped with a PpL affinity col-umn (Cat ...
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bioRxiv - Cell Biology 2019Quote: ... and pCMV-VSV-G-RSV-Rev were co-transfected into Lenti-X 293T cells using polyethylenimine (no. 24765-1, Polyscience Inc., Warrington, PA). To prepare the retrovirus ...
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bioRxiv - Cell Biology 2021Quote: ... and pCMV-VSV-G-RSV-Rev were co-transfected into Lenti-X 293T cells using polyethylenimine (no. 24765-1, Polyscience Inc., Warrington, PA). The infected cells were selected with media containing the following antibiotics ...
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bioRxiv - Biophysics 2024Quote: ... and a fresh 1:10,000 dilution of 1 µm beads (Polybead® Microspheres 1 µm, Polyscience, Inc) in medium was added to the sample ...
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bioRxiv - Neuroscience 2019Quote: ... Toluidine blue was performed on sciatic nerve fixed in phosphate buffer 2% glutaraldehyde (Polyscience cat.07710) to assess the number of axons and the myelin thickness ...
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bioRxiv - Biophysics 2023Quote: ... Previously 1 μm beads (Polybead® Microspheres 1 μm, Polyscience, Inc) were added in 1:10,000 dilution to the medium ...
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bioRxiv - Neuroscience 2022Quote: Transient transfection in Cos-7 cells or Neuro2A cells was performed using Polyethylenimine HCl Max (Polyscience). After transfection for 20 h ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells growing in 6-well plates (2.5×105 cells/well) were transfected using linear PEI ~25000 Da (Polyscience) as described previously24 Cells were transfected using a ratio μg DNA/μl PEI of 1/15 and the DNA/PEI mix was incubated for 20 min at room temperature before adding to the cells ...
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bioRxiv - Microbiology 2023Quote: HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
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bioRxiv - Biophysics 2023Quote: ... We transfected HeLa cells (ATCC CCL-2) with 10 µg of each px459 v2.0 plasmid together with 40 µl of linear PEI (Polyscience 23966) using standard protocols ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were mounted in Aqua polymount (Polyscience) and coverslips were sealed with nail-polish to prevent hardening of the mounting media ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAG-Sema6a-GFP or pCAG-Sema6aΔcyt-GFP constructs in 1:3 PEI 1 mg/ml (Polyscience) in H2O ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL polyethylenimine (PEI, Polyscience) in PBS was used for the transfection at a ratio of 3:1 (μL:μg ...
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bioRxiv - Molecular Biology 2019Quote: 293T cells were transfected with overexpression constructs along with packaging vectors VSV-G and Delta8.9 using PEI reagent (PEI MAX, Polyscience #24765-2). Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using polyethylenimine (PEI; Polyscience, 24765) dissolved in PBS to 1 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... HEK 293T cells were transfected using polyethyleneimine (Polyscience Inc.) with packaging plasmids pRSVrev ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected using polyethyleneimine MAX (PEI Max) (Polyscience). The amount of the plasmids and PEI Max were optimized in proportion to the relative surface area and the number of cells ...
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bioRxiv - Cell Biology 2021Quote: ... 4.5 μl X-tremeGENE 9 in 100 μl serum-free OptiMEM) or PEI transfection reagent (1 mg ml−1 stock, Polyscience 24765-2 ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were mounted on microscope slides in Aqua polymount (Polyscience). Primary antibodies used ...
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bioRxiv - Biophysics 2023Quote: ... Probe particles (Polybead® Microspheres 1 μm, Polyscience, Inc) were suspended in 60% Glycerol (ROTIPURAN® ...
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bioRxiv - Molecular Biology 2021Quote: Cell transfections were performed using polyethyleneimine (PEI) reagent (Polyscience Inc., 23966) or jetPRIME (Polyplus transfection ...
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bioRxiv - Cell Biology 2020Quote: Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.2 µL of 1 mg/mL PEI MAX (Polyscience #24765), and 100 µL of 1x PBS were mixed ...