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Citations for PolyScience :
1 - 50 of 52 citations for LIR 1 LILRB1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... HEK293-F were transfected with polyethylenimine (Polyscience Europe GmbH) at a ratio of 1:3 DNA:polyethylenimine (Tom et al. ...
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bioRxiv - Microbiology 2021Quote: ... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: The heavy and light chain plasmids were then co-transfected into HEK293 cells us-ing PEI MAX® (Cat: 24765-1, PolyScience). The production supernatant was harvested af-ter 14 days post-transfection and purified using AKTA equipped with a PpL affinity col-umn (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were transfected with expression and packaging plasmids following standard calcium phosphate or polyethylenimine (Polyscience, 23966) protocols ...
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bioRxiv - Immunology 2021Quote: ... pαH-MERS RBD-Fc using 2 mg of polyethyleneimine (Polyscience, Cat# 24765). After 6 days of growth ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were transiently transfected by endotoxin free construct of MHV-Fc virolectin or its mutant in presence of polyethyleneimine (PEI, Polyscience Inc, 23966). Precisely ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors were transiently transfected to human HEK293F cells with polyethylenimine (Polyscience). Three days later ...
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bioRxiv - Molecular Biology 2022Quote: ... CHRD and human CAP1 expression vectors (250 μg) using polyethylenimine (750 μg; Polyscience). Transfected cells were cultured at 37°C under 6% CO2 on an orbital shaker platform rotating at 135 rpm for 7 days before harvesting ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with pLIB2-BSD plasmid bearing cDNA of human CLPTM1L or CLPTM1 by using PEI “Max” transfection reagent (Polyscience) and cultured for 12 hours ...
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bioRxiv - Biophysics 2024Quote: ... and a fresh 1:10,000 dilution of 1 µm beads (Polybead® Microspheres 1 µm, Polyscience, Inc) in medium was added to the sample ...
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bioRxiv - Biophysics 2023Quote: ... Previously 1 μm beads (Polybead® Microspheres 1 μm, Polyscience, Inc) were added in 1:10,000 dilution to the medium ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAG-Sema6a-GFP or pCAG-Sema6aΔcyt-GFP constructs in 1:3 PEI 1 mg/ml (Polyscience) in H2O ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL polyethylenimine (PEI, Polyscience) in PBS was used for the transfection at a ratio of 3:1 (μL:μg ...
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bioRxiv - Cell Biology 2021Quote: ... 4.5 μl X-tremeGENE 9 in 100 μl serum-free OptiMEM) or PEI transfection reagent (1 mg ml−1 stock, Polyscience 24765-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... or pcDNA3.1-FLAG-hSF3B1-K700E and 0.5 µg of the respective minigene construct using the ratio 1:2 of DNA:PEI MAX (PolyScience, #24765-1) according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2023Quote: ... Probe particles (Polybead® Microspheres 1 μm, Polyscience, Inc) were suspended in 60% Glycerol (ROTIPURAN® ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded into multi-well plates and transfected with polyethyleneimine (PEI, 1 mg/ml in H2O, pH 7, Polyscience, catalog no. 23966-1). Therefore ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.2 µL of 1 mg/mL PEI MAX (Polyscience #24765), and 100 µL of 1x PBS were mixed ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained with DAPI (1:1000) and mounted with AquaPolymount (Polyscience). High-resolution images dendritic spines were acquired using a Nikon A1 Eclipse confocal microscope equipped with NIS element AR v4.20.03 software (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... Ten µg of total DNA in 1 mL of plain optiMEM was mixed with 40 µl of Polyethyleneimine “Max” (PEI, Polyscience #24765, 1 mg/mL in dH2O), briefly vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected using PEI (23966-1, Polyscience, Warrington, PA) with the pLentiCRISPRv2-LoxPv1 gRNA containing plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... and an ITR-flanked transgene using polyethylenimine (PEI, Polyscience, 24765-1). AAVs were harvested from the cells and the media 3 days post transfection and purified by ultracentrifugation over iodixanol gradients ...
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bioRxiv - Cell Biology 2023Quote: ... 300 µL of 1 mg/mL polyethylenimine linear 25k (Polyscience, 23966) was diluted into 5 mL OptiMEM in a separate tube ...
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bioRxiv - Neuroscience 2023Quote: ... blue fluorescent neuronal tracer fast blue (Polyscience Inc, 17740-1, 1mg) onto the surface of L1 for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... we used the PEI transfection reagent (Linear PEI25K, PolyScience; 23966-1). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 µg lentiviral plasmid using PEI-Max (Polyscience, cat.no. 24765-1). Lentiviral supernatant was harvested 36 ...
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bioRxiv - Bioengineering 2023Quote: ... One (1) μm yellow PSP and 6 μm red PSP were from Polyscience Inc ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection of HEK293T cells was carried out using 1 mg/ml Polyethyleneimine MAX (Polyscience). Mycoplasma contamination was monitored by DAPI staining.
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded onto assay plates and transfected using polyethyleneimine (PEI, Polyscience, 1 mg/mL). Details on the conditions for each experiment can be found in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then transfected with 1μg sgRNA plasmid by using 1 mg/ml Polyethyleneimine MAX (Polyscience). Puromycin was used to selecte the transfected cells ...
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bioRxiv - Developmental Biology 2021Quote: ... En bloc staining was done in the dark with 1% UA (cat# 21447, Polyscience Europe GmbH) in water at 4°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... along with a 1 µl suspension containing M9 and 20 µm diameter poly-styrene microspheres (Polyscience, Warrington, PA). The preparation was then covered with another coverslip and sealed using vaseline ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
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bioRxiv - Microbiology 2023Quote: ... DNA (1 µg/ml final transfection volume) was added followed by addition of linear polyethylenimine (PEI25k, Polyscience, Inc.) (1mg/ml ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were cotransfected with pcDNA3.1-Env clone and Env-deficient HIV-1 backbone vector (pSG3△Env) using PEI transfection reagent (PolyScience). Pseudovirus-containing supernatant was harvested 48h post-transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... for 24 hours after which transfection took place using 187.5 ng total DNA per well and 1 μg/μl PEI-MAX (Polyscience) mixed with Opti-MEM medium (Gibco) ...
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bioRxiv - Immunology 2019Quote: ... for IgG was mixed with 25 mL Opti-MEM containing 2,250 µg polyethylene imine MAX (MW 40,000; Polyscience - 24765-1). After incubation for 20 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... This plasmid was then transfected into immortalized STING-knockout MEFs (Mukai et al., 2016) with PEI MAX (24765-1, Polyscience). Twenty-four hours after transfection ...
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bioRxiv - Bioengineering 2022Quote: ... then Omicron spike protein expression vectors were combined with HIV-1 structural corresponding plasmids and co-transfected into HEK-293T cells with PEI (1mg/ml, PEI MAX, Polyscience). Two-day post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and a total of 800 ng of plasmids encoding both Target-AID and gRNA were combined with 2.5 µL of 1 mg/mL PEI MAX (Polyscience #24765) and 200 µL of 1x PBS and applied to each well after 5–10 min incubation at room temperature.
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bioRxiv - Bioengineering 2023Quote: ... cells were transiently transfected at a concentration of 140 ng total DNA per well and 1 μg/μl PEI-MAX (Polyscience) mixed with Opti-MEM medium (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... For the retrograde tracing of lung-innervating VG neurons mice received a volume of 40 µl Fast Blue solution (350 µM and 1% DMSO in 0.9% saline, 73819-41-7, Polyscience Inc) or 40 µl DiD solution (0.5 m/ml in 0.9% saline ...
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bioRxiv - Immunology 2023Quote: ... and all plasmid constructs used throughout the study were transfected into cells using PEI transfection reagent (Polyscience, Cat; 24765-1) at a concentration of 1□µg/ml ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: ... or 100 pmol/mL of SC-siRNA and linear polyethylenimine (PEI) with a molecular weight of 25 kDa (23966-1; Polyscience, USA) in a 1:3 ratio (μg of siRNA ...
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bioRxiv - Molecular Biology 2024Quote: 1.6 µg of expression plasmid DNA in 100 µl of 150 mM NaCl were mixed with 100 µl of 150 mM NaCl supplemented with 7 µl of 1 mg/ml PEI (polyethylenimine ‘MAX’ Polyscience, Inc.). After 30 min incubation at a room temperature ...
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bioRxiv - Genetics 2023Quote: ... 15 μg DNA was incubated in 600 μL Opti-MEM and 60 μL of 1 mg/mL linear PEI (Polyscience, 23966-2) for 15 minutes at room temperature before adding to cells dropwise ...
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bioRxiv - Neuroscience 2023Quote: ... and the pHGT1-Adeno1 helper plasmid carrying adenoviral genes (kindly provided by C. Cepko, Harvard Medical School, Boston, USA) using PEIMAX (Polyscience, #POL24765-1). Plasmids were mixed in 98 mL DMEM (Thermo Fischer ...