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Citations for PolyScience :
1 - 50 of 76 citations for 7 cyano 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... ∼2 × 107 3-μm latex NH2-beads (Polyscience) were activated with 8% glutaraldehyde for 4 h at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded into multi-well plates and transfected with polyethyleneimine (PEI, 1 mg/ml in H2O, pH 7, Polyscience, catalog no. 23966-1). Therefore ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAG-Sema6a-GFP or pCAG-Sema6aΔcyt-GFP constructs in 1:3 PEI 1 mg/ml (Polyscience) in H2O ...
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bioRxiv - Neuroscience 2023Quote: ... For the retrograde tracing of lung-innervating VG neurons mice received a volume of 40 µl Fast Blue solution (350 µM and 1% DMSO in 0.9% saline, 73819-41-7, Polyscience Inc) or 40 µl DiD solution (0.5 m/ml in 0.9% saline ...
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bioRxiv - Molecular Biology 2024Quote: 1.6 µg of expression plasmid DNA in 100 µl of 150 mM NaCl were mixed with 100 µl of 150 mM NaCl supplemented with 7 µl of 1 mg/ml PEI (polyethylenimine ‘MAX’ Polyscience, Inc.). After 30 min incubation at a room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-4 cell stage embryos were mounted between two cover slips in M9 buffer containing 20 μm microspheres (Polyscience). Dual-color 3D time-lapse imaging was performed using a Spinning disc confocal microscopy ...
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bioRxiv - Biochemistry 2022Quote: ... Temperature was kept at 30 °C using a water bath (PolyScience 7 L, IL). Oxygen consumption reaction mixture was MES 10 mM pH 6 and 500 mg (wet weight ...
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... or pcDNA3.1-FLAG-hSF3B1-K700E and 0.5 µg of the respective minigene construct using the ratio 1:2 of DNA:PEI MAX (PolyScience, #24765-1) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...
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bioRxiv - Neuroscience 2022Quote: Transient transfection in Cos-7 cells or Neuro2A cells was performed using Polyethylenimine HCl Max (Polyscience). After transfection for 20 h ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 μl Concavalin-A slurry (Polyscience, 86057-3) per sample were activated by washing the bead slurry three times in 1.5 mL Binding Buffer (HEPES pH 7.5 20 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
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bioRxiv - Biochemistry 2022Quote: These experiments were carried out using a stopped-flow spectrometer (SX20, Applied Photophysics) equipped with a 7-liter refrigerated circulator (Polyscience) at 22 °C ...
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bioRxiv - Genomics 2023Quote: ... Polyethylenimine (PEI) (Polyscience 23966-2) was added at a 3:1 PEI:DNA ratio and the mixture briefly vortexed and incubated at RT for 10-20 mins ...
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bioRxiv - Cell Biology 2020Quote: ... ~2x 107 3-μm latex NH2-beads (Polyscience, Eppelheim, Germany) were activated with 8% glutaraldehyde for four h at room temperature ...
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bioRxiv - Genomics 2021Quote: ... Heating to 25°C was achieved using 200-watt electric heaters and cooling to 7°C was done using a refrigerated/heated 6L circulating bath (PolyScience, 9106A11B). Controls were kept at 15°C but handled in the same manner as the 25°C and 7°C groups ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 4% v/v formaldehyde (methanol-free; Polyscience) for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... Transfection was conducted with 4 μl of PEI (Polyscience) following the manufacturer’s manual and 1μg of CR plasmids and 1μg of rgRNA plasmids ...
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bioRxiv - Genetics 2023Quote: ... 15 μg DNA was incubated in 600 μL Opti-MEM and 60 μL of 1 mg/mL linear PEI (Polyscience, 23966-2) for 15 minutes at room temperature before adding to cells dropwise ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNAs were introduced into Lenti-X 293T or HEK293T cells using either polyethylenimine hydrochloride (PEI) (49553-93-7, Polyscience, Niles, IL, USA) or TransIT®-293 Transfection Reagent (MIR2700 ...
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bioRxiv - Cell Biology 2023Quote: ... root meristems were excised and fixed in 4% formaldehyde (Polyscience) in PBS pH 7.2 for 24 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... contrasted with 2% uranyl acetate (Polyscience, 21447) and lead citrate(Reynold ...
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bioRxiv - Cell Biology 2020Quote: Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
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bioRxiv - Neuroscience 2022Quote: ... were cut on the ultramicrotome with a diamond knife (Jumdi, 2 mm) and then collected onto TEM copper grids (Gilder) and stained with 2% uranyl acetate (Polyscience) and 1% lead citrate (Reynolds 1963) ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were fixed in 2 % paraformaldehyde (Polyscience; Hirschberg an der Bergstrasse ...
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bioRxiv - Developmental Biology 2024Quote: ... elegans were mounted on 2% agarose (w/v in water) dry pads immersed in 2% polystyrene bead (0.1 mm diameter, Polyscience, 00876-15) diluted in M9 buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Forty microliters of 2 mg/mL PEI “Max” (Polyscience) was diluted in 1.25 mL of OptiMEM and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Microbiology 2022Quote: ... Expi293 cells were grown to 3×106/mL before transfection with the indicated spike gene using Polyethylenimine (Polyscience). Cells were cultured overnight at 37°C with 8% CO2 and VSV-G pseudo-typed ΔG-luciferase (G*ΔG-luciferase ...
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bioRxiv - Developmental Biology 2024Quote: ... mounted in a 3 μL drop of M9 containing approximately fifty 25 μm diameter polystyrene beads (Polyscience Inc.) and sealed under a coverslip using Vaseline ...
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bioRxiv - Immunology 2021Quote: ... pαH-MERS RBD-Fc using 2 mg of polyethyleneimine (Polyscience, Cat# 24765). After 6 days of growth ...
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bioRxiv - Molecular Biology 2023Quote: ... Rev and Gag-pol using PEI reagent (PEI MAX, Polyscience, 24765-2). The supernatant was collected after 48 and 72 h ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected using the PEImax (Cat# 24765-2, MW 40,000, Polyscience, Inc.) at a ratio of 1:3 of DNA ...
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bioRxiv - Biophysics 2024Quote: ... and a fresh 1:10,000 dilution of 1 µm beads (Polybead® Microspheres 1 µm, Polyscience, Inc) in medium was added to the sample ...
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bioRxiv - Neuroscience 2019Quote: ... Toluidine blue was performed on sciatic nerve fixed in phosphate buffer 2% glutaraldehyde (Polyscience cat.07710) to assess the number of axons and the myelin thickness ...
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bioRxiv - Biophysics 2023Quote: ... Previously 1 μm beads (Polybead® Microspheres 1 μm, Polyscience, Inc) were added in 1:10,000 dilution to the medium ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with physiological saline followed by an ice-cold 0.1M phosphate-buffered (pH 7.4) fixative containing 4% PFA and 0.1% glutaraldehyde (Polyscience Europe GmbH, Eppelheim, Germany) for 15min ...
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bioRxiv - Microbiology 2023Quote: HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
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bioRxiv - Biophysics 2023Quote: ... We transfected HeLa cells (ATCC CCL-2) with 10 µg of each px459 v2.0 plasmid together with 40 µl of linear PEI (Polyscience 23966) using standard protocols ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL polyethylenimine (PEI, Polyscience) in PBS was used for the transfection at a ratio of 3:1 (μL:μg ...
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bioRxiv - Molecular Biology 2019Quote: 293T cells were transfected with overexpression constructs along with packaging vectors VSV-G and Delta8.9 using PEI reagent (PEI MAX, Polyscience #24765-2). Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG ...
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bioRxiv - Cell Biology 2021Quote: ... 4.5 μl X-tremeGENE 9 in 100 μl serum-free OptiMEM) or PEI transfection reagent (1 mg ml−1 stock, Polyscience 24765-2 ...
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bioRxiv - Biophysics 2023Quote: ... Probe particles (Polybead® Microspheres 1 μm, Polyscience, Inc) were suspended in 60% Glycerol (ROTIPURAN® ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.2 µL of 1 mg/mL PEI MAX (Polyscience #24765), and 100 µL of 1x PBS were mixed ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained with DAPI (1:1000) and mounted with AquaPolymount (Polyscience). High-resolution images dendritic spines were acquired using a Nikon A1 Eclipse confocal microscope equipped with NIS element AR v4.20.03 software (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... Ten µg of total DNA in 1 mL of plain optiMEM was mixed with 40 µl of Polyethyleneimine “Max” (PEI, Polyscience #24765, 1 mg/mL in dH2O), briefly vortexed and incubated at room temperature for 15 min ...