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Citations for PolyScience :
1 - 50 of 59 citations for 6 Oxa 7 azatricyclo 3.3.0.02 4 oct 7 ene 8 1 methylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... HEK293 cells were transiently co-transfected at a ratio of 1:2 (H:L) with PEI (49553-93-7, Polyscience) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... For the retrograde tracing of lung-innervating VG neurons mice received a volume of 40 µl Fast Blue solution (350 µM and 1% DMSO in 0.9% saline, 73819-41-7, Polyscience Inc) or 40 µl DiD solution (0.5 m/ml in 0.9% saline ...
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bioRxiv - Biochemistry 2022Quote: ... Temperature was kept at 30 °C using a water bath (PolyScience 7 L, IL). Oxygen consumption reaction mixture was MES 10 mM pH 6 and 500 mg (wet weight ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were seeded into multi-well plates and transfected with polyethyleneimine (PEI, 1 mg/ml in H2O, pH 7, Polyscience, catalog no. 23966-1). Therefore ...
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bioRxiv - Molecular Biology 2024Quote: 1.6 µg of expression plasmid DNA in 100 µl of 150 mM NaCl were mixed with 100 µl of 150 mM NaCl supplemented with 7 µl of 1 mg/ml PEI (polyethylenimine ‘MAX’ Polyscience, Inc.). After 30 min incubation at a room temperature ...
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bioRxiv - Neuroscience 2022Quote: Transient transfection in Cos-7 cells or Neuro2A cells was performed using Polyethylenimine HCl Max (Polyscience). After transfection for 20 h ...
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bioRxiv - Biochemistry 2022Quote: These experiments were carried out using a stopped-flow spectrometer (SX20, Applied Photophysics) equipped with a 7-liter refrigerated circulator (Polyscience) at 22 °C ...
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bioRxiv - Genomics 2021Quote: ... Heating to 25°C was achieved using 200-watt electric heaters and cooling to 7°C was done using a refrigerated/heated 6L circulating bath (PolyScience, 9106A11B). Controls were kept at 15°C but handled in the same manner as the 25°C and 7°C groups ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNAs were introduced into Lenti-X 293T or HEK293T cells using either polyethylenimine hydrochloride (PEI) (49553-93-7, Polyscience, Niles, IL, USA) or TransIT®-293 Transfection Reagent (MIR2700 ...
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bioRxiv - Bioengineering 2023Quote: ... One (1) μm yellow PSP and 6 μm red PSP were from Polyscience Inc ...
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bioRxiv - Microbiology 2020Quote: ... Individual plasmids or a combination of the gH and gL expression plasmids (4:1 ratio) were transfected into HEK293T cells using polyethylenimine (PolyScience) as described previously (33) ...
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bioRxiv - Physiology 2020Quote: ... Polystyrene black dyed microspheres (diameter 6 μm, 2.6% solid-latex, Polybead, Polyscience Inc., Warrington, PA) were washed and diluted with physiological solution (0,5% latex ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 4% v/v formaldehyde (methanol-free; Polyscience) for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... Transfection was conducted with 4 μl of PEI (Polyscience) following the manufacturer’s manual and 1μg of CR plasmids and 1μg of rgRNA plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... root meristems were excised and fixed in 4% formaldehyde (Polyscience) in PBS pH 7.2 for 24 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Infiltrated cochleae were then incubated in pure resin for 8-12 hours before being placed in embedding molds (Polyscience, Germany) and incubated in a pre-warmed oven at 70°C for at least 72 hours.
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bioRxiv - Cell Biology 2020Quote: Cells grown on coverslips were fixed with 4% formaldehyde (Polyscience, 18814) for 20⍰min in room temperature and permeabilized with 0.05% Saponin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells growing in 6-well plates (2.5×105 cells/well) were transfected using linear PEI ~25000 Da (Polyscience) as described previously24 Cells were transfected using a ratio μg DNA/μl PEI of 1/15 and the DNA/PEI mix was incubated for 20 min at room temperature before adding to the cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 2-4 cell stage embryos were mounted between two cover slips in M9 buffer containing 20 μm microspheres (Polyscience). Dual-color 3D time-lapse imaging was performed using a Spinning disc confocal microscopy ...
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bioRxiv - Biophysics 2024Quote: ... and a fresh 1:10,000 dilution of 1 µm beads (Polybead® Microspheres 1 µm, Polyscience, Inc) in medium was added to the sample ...
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bioRxiv - Biophysics 2023Quote: ... Previously 1 μm beads (Polybead® Microspheres 1 μm, Polyscience, Inc) were added in 1:10,000 dilution to the medium ...
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bioRxiv - Neuroscience 2020Quote: ... and then transcardially perfused with physiological saline followed by an ice-cold 0.1M phosphate-buffered (pH 7.4) fixative containing 4% PFA and 0.1% glutaraldehyde (Polyscience Europe GmbH, Eppelheim, Germany) for 15min ...
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bioRxiv - Developmental Biology 2023Quote: ... pCAG-Sema6a-GFP or pCAG-Sema6aΔcyt-GFP constructs in 1:3 PEI 1 mg/ml (Polyscience) in H2O ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL polyethylenimine (PEI, Polyscience) in PBS was used for the transfection at a ratio of 3:1 (μL:μg ...
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bioRxiv - Cell Biology 2021Quote: ... 4.5 μl X-tremeGENE 9 in 100 μl serum-free OptiMEM) or PEI transfection reagent (1 mg ml−1 stock, Polyscience 24765-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... or pcDNA3.1-FLAG-hSF3B1-K700E and 0.5 µg of the respective minigene construct using the ratio 1:2 of DNA:PEI MAX (PolyScience, #24765-1) according to the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2023Quote: ... Probe particles (Polybead® Microspheres 1 μm, Polyscience, Inc) were suspended in 60% Glycerol (ROTIPURAN® ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1.2 µL of 1 mg/mL PEI MAX (Polyscience #24765), and 100 µL of 1x PBS were mixed ...
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bioRxiv - Neuroscience 2024Quote: ... counterstained with DAPI (1:1000) and mounted with AquaPolymount (Polyscience). High-resolution images dendritic spines were acquired using a Nikon A1 Eclipse confocal microscope equipped with NIS element AR v4.20.03 software (Nikon ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfection of cells with plasmid DNA was performed on 80 % confluent cells using PEI-Max (Polyscience, cat.no. 24765-1, DNA:PEI ratio of 1:3) for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... Ten µg of total DNA in 1 mL of plain optiMEM was mixed with 40 µl of Polyethyleneimine “Max” (PEI, Polyscience #24765, 1 mg/mL in dH2O), briefly vortexed and incubated at room temperature for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected using PEI (23966-1, Polyscience, Warrington, PA) with the pLentiCRISPRv2-LoxPv1 gRNA containing plasmid ...
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bioRxiv - Neuroscience 2022Quote: ... and an ITR-flanked transgene using polyethylenimine (PEI, Polyscience, 24765-1). AAVs were harvested from the cells and the media 3 days post transfection and purified by ultracentrifugation over iodixanol gradients ...
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bioRxiv - Cell Biology 2023Quote: ... 300 µL of 1 mg/mL polyethylenimine linear 25k (Polyscience, 23966) was diluted into 5 mL OptiMEM in a separate tube ...
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bioRxiv - Neuroscience 2023Quote: ... blue fluorescent neuronal tracer fast blue (Polyscience Inc, 17740-1, 1mg) onto the surface of L1 for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... we used the PEI transfection reagent (Linear PEI25K, PolyScience; 23966-1). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 µg lentiviral plasmid using PEI-Max (Polyscience, cat.no. 24765-1). Lentiviral supernatant was harvested 36 ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection of HEK293T cells was carried out using 1 mg/ml Polyethyleneimine MAX (Polyscience). Mycoplasma contamination was monitored by DAPI staining.
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bioRxiv - Neuroscience 2023Quote: ... 1-2 μL PCR product mixed with 0.9 μL PEI MAX (Polyscience, 24765-100) was directly transfected into HEK293T cells in the 96-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded onto assay plates and transfected using polyethyleneimine (PEI, Polyscience, 1 mg/mL). Details on the conditions for each experiment can be found in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then transfected with 1μg sgRNA plasmid by using 1 mg/ml Polyethyleneimine MAX (Polyscience). Puromycin was used to selecte the transfected cells ...
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bioRxiv - Developmental Biology 2021Quote: ... En bloc staining was done in the dark with 1% UA (cat# 21447, Polyscience Europe GmbH) in water at 4°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... along with a 1 µl suspension containing M9 and 20 µm diameter poly-styrene microspheres (Polyscience, Warrington, PA). The preparation was then covered with another coverslip and sealed using vaseline ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were transfected using 340 μL of polyethylenimine (PolyScience, cat. #24765-2, diluted to 1 mg/mL) with the following DNA plasmids ...
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bioRxiv - Microbiology 2023Quote: ... DNA (1 µg/ml final transfection volume) was added followed by addition of linear polyethylenimine (PEI25k, Polyscience, Inc.) (1mg/ml ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were cotransfected with pcDNA3.1-Env clone and Env-deficient HIV-1 backbone vector (pSG3△Env) using PEI transfection reagent (PolyScience). Pseudovirus-containing supernatant was harvested 48h post-transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... for 24 hours after which transfection took place using 187.5 ng total DNA per well and 1 μg/μl PEI-MAX (Polyscience) mixed with Opti-MEM medium (Gibco) ...
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bioRxiv - Immunology 2019Quote: ... for IgG was mixed with 25 mL Opti-MEM containing 2,250 µg polyethylene imine MAX (MW 40,000; Polyscience - 24765-1). After incubation for 20 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... This plasmid was then transfected into immortalized STING-knockout MEFs (Mukai et al., 2016) with PEI MAX (24765-1, Polyscience). Twenty-four hours after transfection ...
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bioRxiv - Bioengineering 2022Quote: ... then Omicron spike protein expression vectors were combined with HIV-1 structural corresponding plasmids and co-transfected into HEK-293T cells with PEI (1mg/ml, PEI MAX, Polyscience). Two-day post-transfection ...