Labshake search
Citations for Cell Biolabs :
1 - 50 of 54 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 p17 1981 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... For production of virus for mouse cells platinum E cells (Cell Biolabs, Cat#RV-101) were transfected ...
-
bioRxiv - Immunology 2022Quote: ... mouse anti-GAPDH (1:1,000; Cat#: AKR-001, Cell Biolabs Inc.), rabbit-anti Influenza A H1N1 HA (1:1,000 ...
-
bioRxiv - Immunology 2022Quote: ... Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs, INC). Mice were infected by tail vein injection with 4x109 viral particles before sacrifice on day 7 for immunofluorescence experiments or on day 10 for all other experiments.
-
bioRxiv - Molecular Biology 2020Quote: ... vectors were titrated using QuickTiter Lentivirus Titer Kit (Lentivirus-Associated HIV p24; Cell Biolabs, San Diego, CA); the viral titers for SHC002 and SHC016 were similar ...
-
bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies (mouse anti-yH2AX, Cell Biolabs, STA-321 ...
-
bioRxiv - Neuroscience 2022Quote: ... Lentivirus titer was determined using the QuickTiter Lentivirus titer kit (lentivirus-associated HIV p24; Cell Biolabs, VPK-107) or HIV-1 p24 ELISA pair set (Sino Biological Inc ...
-
bioRxiv - Bioengineering 2021Quote: ... The number of lentiviral particles were quantified using the QuikTiter Lentivirus Quantitation Kit from Cell Biolabs (HIV P24 ELISA). All reported viral titers were within the linear range of the standard curve made using a positive control ...
-
bioRxiv - Molecular Biology 2021Quote: ... and co-transfected with pAAV-DJ vector and pAAV-Helper vector into HEK 293T cells to generate recombinant adeno-associated virus expressing Mir20b or anti-Mir20b according to the manufacturer’s protocol (Cell Biolabs, San Diego, CA). The AAVs were purified with AAVpro® Purification Kit (Takara Bio ...
-
bioRxiv - Cell Biology 2021Quote: ... Virus was produced by transfecting Platinum-E retroviral packaging cells (Cell Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Virus was titered using the QuickTiter™ Lentivirus Titer Kit (Cell Biolabs, Inc) following manufacturer conditions.
-
bioRxiv - Neuroscience 2020Quote: AAV virus was made using the AAV-DJ Helper Free Packaging System (Cell Biolabs) using published protocols95 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were verified to be free of residual viral particles 10 days post transduction using the QuickTiter™ Lentivirus-Associated HIV p24 titer kit (Cell BioLabs Inc.).
-
bioRxiv - Neuroscience 2021Quote: Adeno-associated virus (AAV) was generated using the AAV-DJ Helper Free system (Cell Biolabs) as published previously9,27 ...
-
bioRxiv - Immunology 2021Quote: Physical particles were determined by measuring p24 levels using the QuickTitre™ Lentivirus Titre which quantifies lentivirus-associated HIV rather than free p24 proteins (Cell Biolabs – VPK-107-T). Manufacturer’s protocol was followed ...
-
bioRxiv - Neuroscience 2019Quote: Adeno-associated virus (AAV) vectors were packaged using the AAV Helper Free Expression System (Cell Biolabs) as described previously45 ...
-
bioRxiv - Cell Biology 2021Quote: ... Virus titer was measured using the QuickTiter™ Lentivirus Titer Kit (Cell Biolabs, VPK-107-T) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: ... The virus packaging cell lines Platinum-E (Plat-E,Cell Biolabs, Inc, San Diego, CA, USA) and GPp210-GFP61 were kept in DMEM (Sigma ...
-
bioRxiv - Genomics 2022Quote: Concentrations of LVs and Cas9 VLPs were determined by LV-associated HIV p24 capsid protein (CA)-based ELISA (QuickTiter™ Lentivirus Titer Kit, Catalog Number VPK-107, Cell Biolabs, Inc. San Diego, CA). LVs or VLPs were precipitated according to the manufacturer’s instructions so that soluble p24 protein was not detected.
-
bioRxiv - Cell Biology 2023Quote: ... and the virus titer was measured based on the titer of the viral protein p24 with QuickTiterTM Lentivirus Titer Kit (Cell Biolabs) following the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2021Quote: ... The plasmid expressing the VSV-G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The lentiviral plasmid used to generate the HEK-293T stable cell lines expressing the human ACE2 gene (GenBank ID NM_021804 ...
-
bioRxiv - Bioengineering 2022Quote: ... The envelope vector expressing VSV-G (vesicular stomatitis virus glycoprotein) was obtained from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). To generate the expression plasmid for D614G mutant of SARS-CoV-2 Spike (S ...
-
bioRxiv - Biochemistry 2021Quote: ... while the plasmid expressing VSV G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The HDM-IDTSpike-fixK plasmid was employed as a template for site-directed mutagenesis to generate the expression plasmid for the D614G mutant of SARS-CoV-2 spike protein ...
-
bioRxiv - Neuroscience 2021Quote: Fibcd1 constructs were packaged into virus by CaCl2 transfecting 70-80% confluent 10cm2 plate of Platinum-E (PlatE) HEK 293T cells (Cell Biolabs #RV-101) with 10µg Fibcd1 construct and 2µg gag/pol plasmid ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal Rac1 (240106; Cell Biolabs) at 1:100 ...
-
bioRxiv - Biophysics 2021Quote: ... and the HEK 293AD human embryonic kidney cell line from Cell Biolabs, Inc (AD-100) ...
-
bioRxiv - Plant Biology 2022Quote: ... Ten micrograms of total protein were separated on 10% SDS- PAGE gel and were followed by protein blot analysis using mouse anti- methylglyoxal monoclonal antibodies (Cell Biolabs, Catalog number- STA-011).
-
bioRxiv - Cell Biology 2024Quote: ... The mouse fibroblast cell line SNL (Cell Biolabs) was used as feeder cells during the reprogramming process of infected NSCs ...
-
bioRxiv - Cancer Biology 2020Quote: ... the human MIR10B gene was cloned into the pMX-IRES-Blasticidin vector (Cell Biolabs Inc., RTV-016), downstream of the constitutive Pol-III U6 promoter ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: HDL was isolated from mouse plasma using HDL Purification Kit (Cell BioLabs, Inc.) following the manufacturer’s protocol ...
-
bioRxiv - Physiology 2023Quote: ... The AAV-HSA-GFP plasmid was developed by inserting a human HSA promoter and GFP (ZsGreen1) into the promoterless AAV vector (Cell BioLabs, Cat. No. VPK-411-DJ) using In-Fusion Cloning (Takara Bio ...
-
bioRxiv - Microbiology 2021Quote: ... incubated overnight with anti-His antibody (Cell Biolabs) and finally with horseradish peroxidase-conjugated goat anti-mouse IgG (Jackson Immuno-research Laboratory ...
-
bioRxiv - Biochemistry 2022Quote: ... The mouse Mafa coding sequence was amplified by PCR and subcloned into pAAV-MCS (VPK-410, Cell Biolabs. Inc.).
-
bioRxiv - Genetics 2020Quote: ... and glucose (#STA-681) in mouse plasma were determined using the appropriate kit (all purchased from Cell Biolabs, Inc). Hearts and whole aortas (from the aortic arch to the iliac artery ...
-
bioRxiv - Biochemistry 2022Quote: ... The FLAG-tagged mouse Pdx1 coding sequence was amplified by PCR and subcloned into a pMXs-Neo Retroviral vector (RTV-011, Cell Biolabs, Inc.). pGL3-basic-MafA plasmid (−10427/+22 from transcription start site ...
-
bioRxiv - Neuroscience 2021Quote: ... The full-length open reading frame of mouse Npgl was amplified from cDNA of the mediobasal hypothalamus and inserted into the pAAV-IRES-GFP expression vector (Cell Biolabs, San Diego, CA). The primers for mouse NPGL were 5’-CGATCGATACCATGGCTGATCCTGGGC-3’ for the sense primer and 5’-CGGAATTCTTATTTTCTCTTTACTTCCAGC-3’ for the antisense primer.
-
bioRxiv - Neuroscience 2020Quote: ... targeting the open reading frame of mouse Tenascin-R into adeno-associated viral (AAV) vector U6 GFP (Cell Biolabs Inc., San Diego, CA 92126, USA), using BamH1 and EcoR1 restriction sites (Okuda et al ...
-
bioRxiv - Neuroscience 2019Quote: ... trans plasmids pAAV2/1 (Cell Biolabs Inc., Cat# VPK-421), pAAV2/2 (Cell Biolabs Inc. ...
-
bioRxiv - Neuroscience 2023Quote: ... Viruses were produced with Serotype 1 or DJ (Cell Biolabs, Inc.) and purified by CsCl gradients 67 ...
-
bioRxiv - Immunology 2023Quote: ... 800 µl of 1 x reagent B (Cell Biolabs; Cat; MET-5011) was added to the cells/ tissues and further incubated on ice for 10 mins with occasional vortexing ...
-
bioRxiv - Cancer Biology 2022Quote: The Plat-A retroviral packaging cell line (1 ξ 106 cells, Cell Biolabs) was seeded on collagen I-coated six-well plates (4810-010 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 μl per 100 μl culture medium of WST-1 reagent (Cell Biolabs, CBA-253) were added to each well ...
-
bioRxiv - Immunology 2021Quote: ... Adhesion of THP-1 cells was measured by Cytoselect Leukocyte-Endothelium Adhesion assay kit (Cell Biolabs, Inc) following suppliers protocol.
-
bioRxiv - Physiology 2022Quote: ... and subsequently incubated with primary antibody against DNP (Cell Biolab; 1:1000 dilution 5% FSG TBS-T) overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... The titres were determined on T-REx™ cells using anti-hexon immunostaining assay based on the QuickTiter™ Adenovirus Titer Immunoassay kit (Cell Biolabs Inc).
-
bioRxiv - Neuroscience 2020Quote: ... EV effects on neuronal membrane integrity were assessed using the ethidium homodimer (EthD-1) assay (Cell Biolabs, San Diego, CA), which is based on the detection of the membrane impermeable EthD-1 dye which fluoresces when bound to DNA ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were then blocked for 1 hour at room temperature in blocking buffer containing 5% PhosphoBLOCKER blocking reagent (Cell Biolabs) in TBST ...
-
bioRxiv - Neuroscience 2024Quote: ... 2017) (Suppl. Table 1) targeting the open reading frame into AAV U6 GFP (Cell Biolabs Inc., San Diego, CA, USA) using BamH1 (New England Biolabs ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 ug of targeting vector was co transfected with 40 ug pDP8 and 200 ul PEI (1 mg/ml) in a P15 of 293AAV cells (AAV-100, Cell biolabs), at 40% confluence ...
-
bioRxiv - Physiology 2022Quote: ... was mixed with collagen solution in a 1:4 ratio of cell suspension and collagen mixture provided in the cell contraction assay (Cell Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: The pri-miR-132 and pri-miR-124 expressing constructs were made by amplifying the pri-miRNA fragments (Supplementary table 1) from miRNASelectTM pEGP-mmu-mirna expression vectors (Cell Biolabs) and subsequently cloning them into the BamHI-BsrGI sites of pRRLSIN.cPPT.SYN.WPRE.