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Citations for Cell Biolabs :
451 - 495 of 495 citations for Dengue Virus Serotype 4 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: Plate-based fluorescent assays using PAR-T ddGFP were performed in a manner similar to the ELISA assays according to the manufacturer’s protocol (Cell Biolabs, XDN-5114). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ROS concentration in culture was assessed using an Oxiselect™ In vitro ROS/RNS Assay kit (Cell Biolabs, San Diego, CA, USA). The experiment was processed following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The FLAG-tagged mouse Pdx1 coding sequence was amplified by PCR and subcloned into a pMXs-Neo Retroviral vector (RTV-011, Cell Biolabs, Inc.). pGL3-basic-MafA plasmid (−10427/+22 from transcription start site ...
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bioRxiv - Bioengineering 2022Quote: ... The senescent phenotype of the irradiation-induced senescent fibroblasts was verified with β-galactosidase staining (following the manufacturers protocol [Cell BioLabs Inc.]) and compared to the control quiescent fibroblasts.
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bioRxiv - Neuroscience 2021Quote: A luciferase reporter driven by a tandem repeat of the Gpr19 CRE sequence (3×CRE-Luc2P) was inserted between the ITR sequences of pAAV-MCS vector (Cell Biolabs Inc) to obtain pAAV-3×CRE-Luc2P ...
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bioRxiv - Immunology 2020Quote: ... by a T2A ribosomal skip sequence and cloned into the pMYs retrovirus backbone under the control of a hybrid MMLV/MSCV promoter (Cell Biolabs Inc). The ffluc sequence was also cloned into the pMYs retrovirus backbone ...
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bioRxiv - Immunology 2020Quote: ... construct.43 The extracellular spacer domain included the murine CD8 hinge region followed by a murine CD8 transmembrane domain.44,45 The intracellular co-stimulatory signaling domain contained the murine 4-1BB followed by a murine CD3ζ cytolytic domain as previously described.46 The CAR sequence was separated from a truncated murine CD19 gene (mCD19t) by a T2A ribosomal skip sequence and cloned into the pMYs retrovirus backbone (Cell Biolabs Inc). Production of retrovirus used to transduce primary murine T cells were performed as previously described.47 The CAR sequence was separated from a truncated murine CD19 gene (mCD19t ...
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bioRxiv - Physiology 2021Quote: ... The AAV-CMV-GFP plasmid was developed by inserting a CMV promoter and GFP (ZsGreen1) into a promoterless AAV vector (Cat. No. VPK-411-DJ; Cell BioLabs, USA) using In-Fusion Cloning (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs (listed in ‘Oligonucleotide sequences’) were synthesized by IDT or Twist Biosciences and cloned into pMXS-IRES-BLAST (Cell Biolabs #RTV-016) linearized with BamHI and NotI using Gibson Assembly (NEB #E2611) ...
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bioRxiv - Physiology 2022Quote: TAG and cholesterol evaluation of tissue and serum were processed according to the manufactures’ instructions (Sigma-Aldrich, MAK266 for TAG and Cell Biolabs, San Diego ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: A LDH release–based cytotoxicity assay was conducted using a CytoSelect LDH Cytotoxicity Assay Kit (CBA-241, Cell Biolabs, Inc., CA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The full cDNA sequence of the lncRNA was then amplified with forward primer: 5’- GTATCATAAGGATCCCTTTCCACTGCTCTGGTGAG-3’ and reverse primer: 5’- GTATCATAAGTCGACCTCACCTAGCTGTCTGTCC-3’ and cloned into pAAV-MCS (Cat#: VPK-410, Cell Biolabs Inc.) using the restriction enzymes BamH I and HindIII sites ...
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bioRxiv - Molecular Biology 2020Quote: ... ELISA was performed according to the manufacturer’s instructions of the OxiselectTM Oxidative DNA Damage ELISA Kit (STA-320, Cell Biolabs Inc., Cologne, Germany), including pretreatment of DNA with Nuclease P1 (N8630 ...
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bioRxiv - Cell Biology 2020Quote: Intracellular accumulation of reactive oxygen species (ROS) was monitored using OxiSelect™ Hydrogen Peroxide/Peroxidase Assay Kit (Cell Biolabs, Inc., San Diego, CA). To investigate ROS generation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were verified to be free of residual viral particles 10 days post transduction using the QuickTiter™ Lentivirus-Associated HIV p24 titer kit (Cell BioLabs Inc.).
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bioRxiv - Microbiology 2022Quote: ... one aliquot was titrated after a single freeze-thaw cycle using the QuickTiter Lentivirus Titer Kit (Cell Biolabs, California, USA. cat. VPK-107) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2022Quote: ... muscle samples were bead homogenized by a Qiagen Tissuelyser in reducing sample buffer (Tris-HCl, pH 6.8; 2% SDS; 10% glycerol; 1% β-mercaptoethanol) containing protease inhibitors (Cell Biolabs, AKR-190). Samples were centrifuged at 10,000 rpm at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Concentrations of cholic acid in liver were quantified using a competitive enzyme immunoassay according to manufacturer recommendations (Cell Biolabs, Inc., catalog MET-5007).
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bioRxiv - Genetics 2020Quote: ... Media containing lentiviruses was collected the next day and viral titers were calculated using QuickTiter Lentivirus Titer Kit (Cell Biolabs, Cat# VPK-107). Titers for lentiviral stock were:
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bioRxiv - Immunology 2022Quote: ... coli particles conjugated with HRP (horseradish peroxidase) in 96-well plates per manufacturer’s protocol (CytoSelect 96-well phagocytosis assay, Cell Biolabs, San Diego CA) for 3 hours in 37°C incubator with 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... AAV-Cap8 vector pDP8 (30 µg; PlasmidFactory GmbH #pF478) or AAV-Cap DJ Rep-Cap vector (30 µg; Cell Biolabs #VPK-420-DJ) using PEI (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Apurinic/apyrimidinic (AP) sites were quantified using the Oxiselect DNA damage ELISA kit (AP sites; STA-324; Cell Biolabs Inc. San Diego, CA, USA), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: A total of 5×105 cells/well were seeded in six-well plates and stained for senescence-associated ß-galactosidase (SA-ß-Gal) activity using the cellular senescence assay kit (Cell Biolabs, Inc.) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... AAV-CMV-YFP-FKBP and AAV-CMV-Tom20-CFP-FRB were produced based on AAV-DJ CMV Expression System (Cell Biolabs, VPK-410-DJ). HEK293T cells were seeded in two six-well cell culture plates (FALCON ...
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bioRxiv - Neuroscience 2021Quote: ... The full-length open reading frame of mouse Npgl was amplified from cDNA of the mediobasal hypothalamus and inserted into the pAAV-IRES-GFP expression vector (Cell Biolabs, San Diego, CA). The primers for mouse NPGL were 5’-CGATCGATACCATGGCTGATCCTGGGC-3’ for the sense primer and 5’-CGGAATTCTTATTTTCTCTTTACTTCCAGC-3’ for the antisense primer.
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bioRxiv - Biochemistry 2019Quote: ... and the sense guides (sgRNA1) into the puromycin-selectable pBABED P U6 plasmid (Dundee-modified version of the original Cell Biolabs pBABE plasmid, DU48788). Cells at about 80% confluency were co-transfected in 10-cm dishes with 2 µg each of the sgRNA plasmid pairs and 20 µl of PEI stock solution (1 mg/ml) ...
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bioRxiv - Physiology 2021Quote: ... and 50 µLof sample was taken to measure the antioxidant capacity using an OxySelect™ TAC Assay kit (using the manufacturer’s instructions) (Cell Biolabs, INC. CA, USA), which is based on the reduction of copper(II ...
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bioRxiv - Cell Biology 2021Quote: The Levels of 4-hydroxynonenal (HNE)-protein adducts of liver lysates prepared in RIPA buffer were measured in livers of mice using the OxiSelect HNE Adduct Competitive ELISA Kit (Cell Biolabs, San Diego, CA), as formerly described 65.
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bioRxiv - Physiology 2022Quote: The adenosine assay was performed using a coupled enzyme reaction according to the method described by the manufacturer (Cell BioLabs, Inc., REF # MET-5090) and adapted from Jagernaath ...
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bioRxiv - Neuroscience 2023Quote: ... AAV-Cap8 vector pDP8 (30 µg; PlasmidFactory GmbH, Germany, #pF478) or AAV-Cap DJ Rep-Cap vector (30 µg; Cell Biolabs, VPK-420-DJ) using PEI (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... ref MS E-5100) and 8-hydroxy-2’-deoxyguanosine (“8-OHdG,” in ng.ml− 1; OxiSelectTM Oxidative DNA Damage Elisa kit, Cell Biolabs Inc., ref STA-320) were measured in duplicates from urine samples as indicators of the organisms’ response to environmental stress (Miller et al. ...
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bioRxiv - Biochemistry 2019Quote: To estimate lipid peroxidation stalk sample malondialdehyde (MDA) content was measured using a TBARS (thiobarbituric acid reactive substances) assay kit (OxiSelect; Cell Biolabs, San Diego, CA, USA). Cellular oxidative stress results in the production of unstable lipid peroxides ...
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bioRxiv - Neuroscience 2019Quote: ... mRNA cDNA clone MGC: 143877 IMAGE:40094330) obtained from Dharmacon (Lafayette. CO) into a pMYs-IRES-GFP retroviral vector (Cell Biolabs Inc, San Diego, CA). Virus expressing the full length Phf15 cDNA or empty vector control were co-transfected with pCL-10 A1 (Addgene plasmid #15805)[30] in HEK293T cells using Lipofectamine 3000 (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... targeting the open reading frame of mouse Tenascin-R into adeno-associated viral (AAV) vector U6 GFP (Cell Biolabs Inc., San Diego, CA 92126, USA), using BamH1 and EcoR1 restriction sites (Okuda et al ...
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bioRxiv - Immunology 2022Quote: ... in serum were determined as indicator of pro-oxidant production using the OxiSelect In Vitro Reactive Oxygen and Nitrogen Species assay kit (Cell BioLabs Inc., San Diego, CA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: The cDNA sequence of the 441 amino acids-long 2N4R Tau isoform was cloned into the pSMPUW-IRES-Bsd vector backbone (Cell Biolabs, cat. no. VPK-219). The empty vector was used as a control ...
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bioRxiv - Neuroscience 2019Quote: ... was introduced via AAV-mediated expression under the synapsin promoter (addgene plasmid #51085 deposited by Jonathan Ting was packaged in-house with the AAV-DJ Helper Free Packaging System of Cell Biolabs; 0.01 µl crude lysate/well). Overexpression of hTau.P301L was induced via AAV-mediated transduction of an expression vector under the synapsin promotor [70,71] and were administered at 3 DIV at MOI 300.
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bioRxiv - Genomics 2019Quote: ... for 120 min at 37 °C and measured by the Boyden chamber method with a 96-well micro-chemotaxis chamber containing a 3-µm pore-sized filter (CELL BIOLABS, INC., San Diego, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: Levels of the cellular senescence marker Senescence Associated-β-Galactosidase (SA-β-Gal) were determined using a modified version of the Quantitative Cellular Senescence Assay kit (Cell Biolabs Inc; CA, U.S.A.). Both undifferentiated and decidualized (C+M treated ...
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bioRxiv - Neuroscience 2020Quote: ... FLAG-Drp1S637A and GFP from the CMV promoter were as previously described (Filippi et al, 2017) using the RAPAd CMV Adenoviral Expression system (Cell Biolabs, Inc. San Diego, CA, USA.), following the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... The AAV-HSA-GFP plasmid was developed by inserting a human HSA promoter and GFP (ZsGreen1) into the promoterless AAV vector (Cell BioLabs, Cat. No. VPK-411-DJ) using In-Fusion Cloning (Takara Bio ...
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bioRxiv - Cancer Biology 2023Quote: ... by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs, catalog number: VPK-206) with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... shown to be effective against Ncan mRNA (Okuda et al., 2014) (Suppl. Table 1) targeting the open reading frame into AAV U6 GFP (Cell Biolabs Inc., San Diego, CA, USA) using BamH1 (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR fragment encoding ERp44 was cloned into the 5′ BamHI and 3′ XhoI sites of the pMXs-IRES-Blasticidin retroviral vector (RTV-016) (Cell Biolabs, Inc., San Diego, CA USA). The following primers were used to produce the C29S mutation of ERp44 using QuikChange mutagenesis ...
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bioRxiv - Neuroscience 2019Quote: ... were generated by the insertion of three different siRNA sequences (obtained from Dharmacon, Horizon Discovery, supplementary table 1) into AAV U6 GFP (CELL BIOLABS INC., San Diego, CA 92126, USA) using BamH1 and EcoR1 restriction sites ...