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Citations for Lucigen :
51 - 96 of 96 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... 5 μL FailSafe 2× PCR premix G (EpiCentre, Madison, Wisconsin), approximately 25 ng genomic DNA template ...
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bioRxiv - Neuroscience 2022Quote: ... Offspring genotypes were confirmed by PCR (Lucigen EconoTaq Plus GREEN 2X) and both heterozygous and homozygous ChAT-cre/jRGECO1a mice were used in the experiments and no phenotypic difference were observed.
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were then in vitro transcribed with T7 polymerase (Lucigen). Capture SELEX was then carried out as follows ...
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bioRxiv - Synthetic Biology 2020Quote: ... Colony PCR was performed as follows: 2X EconoTaq Master mix (Lucigen) was used in 20 µL PCR reactions consisting of 10µL of 2X EconoTaq Master mix (Lucigen) ...
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bioRxiv - Genetics 2020Quote: ... using their associated buffers or alternatively FailSafe PCR PreMix buffers (Epicentre). Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR was carried out using EconoTaq PLUS GREEN 2x master mixes (Lucigen) and primers for target genes and control housekeeping genes ...
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bioRxiv - Biophysics 2023Quote: ... was generated by OE PCR using EconoTaq PLUS 2x Master Mix (Lucigen) with primers listed in Table S9 ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in PCR master mix (EconoTaq PLUS 2× Master Mix, Lucigen), the DNA was amplified for 15 cycles and purified (QIAGEN) ...
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bioRxiv - Neuroscience 2024Quote: ... PCR genotyping was performed using the EconoTaq Plus Green 2X Master Mix (Lucigen) and primer pairs for wild-type and Chd4 floxed alleles (Table 1) ...
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bioRxiv - Biophysics 2019Quote: ... The PCR products were sub-cloned into pGCBlue (Lucigen, pGCBlue Cloning and Amplification kit) or pMiniT (NEB PCR cloning kit ...
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bioRxiv - Bioengineering 2020Quote: ... in 10 μL PCR reactions consisting of 5μL of 2X EconoTaq Master mix (Lucigen), 1uL of each primer (10μM) ...
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bioRxiv - Bioengineering 2021Quote: ... PCR fragments were then in vitro transcribed using the AmpliScribe T7-Flash kit (Lucigen) with overnight incubation at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: Then the PCR products were ligated into vector pSmart LC Kn (Lucigen, cat.# 40821) and electroporated into E ...
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bioRxiv - Genetics 2020Quote: ... for qRT-PCR and northern blotting and MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) for RNA-seq ...
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bioRxiv - Genetics 2020Quote: ... for qRT-PCR and northern blotting and MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) for RNA-seq ...
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bioRxiv - Molecular Biology 2021Quote: Eluted cDNA was moved to a new PCR tube and mixed with CircLigase II (Epicentre) reaction mix containing ...
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bioRxiv - Microbiology 2023Quote: ... each DNA sample was amplified in triplicate using the FailSafe™ PCR System (Epicentre, WI) and the 515FB-806RB primer pair to generate a 400 bp amplicon from the V4 variable regions of the 16S rRNA gene ...
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bioRxiv - Genomics 2021Quote: The DNA libraries for Illumina sequencing were prepared using a Shotgun PCR-free library preparation (Lucigen) Illumina Library at the McGill University and Genome Quebec Innovation Center (Montréal ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were ligated into the pETite vector containing an N-terminal His6 tag (Lucigen) and transformed into HI-Control 10G cells ...
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bioRxiv - Synthetic Biology 2020Quote: ... was used in 20 µL PCR reactions consisting of 10µL of 2X EconoTaq Master mix (Lucigen), 1uL of each primer (10uM concentration) ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and positive colonies were sequenced ...
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bioRxiv - Microbiology 2022Quote: ... difficile colonies on TCCFA were transferred to 25 µL of PCR-Lyse™ (Epicentre, Madison, WI, USA) solution ...
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bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...
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bioRxiv - Microbiology 2019Quote: ... 10.5 µL PCR-grade water and 12.5 µL EconoTaq® PLUS Green 2x Master Mix (Lucigen, Middleton, USA). The cycling conditions were 95 °C for 3 min ...
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bioRxiv - Biochemistry 2021Quote: ... Linear PCR products were ligated by blunt-end ligation using the Fast-link DNA ligation kits from Epicentre. 5μl of the ligation reaction were used for the transformation of chemically-competent E ...
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bioRxiv - Genomics 2021Quote: Contents of a 25 µL PCR mixture included 12.5 µL of EconoTaq Plus Green 2X Master Mix (Lucigen), 0.5 µL of 20 µM of forward and reverse primers ...
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bioRxiv - Bioengineering 2023Quote: ... a PCR-amplified NLuc gene block was transcribed using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, ASF-3507) following manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... a PCR-free library was prepared with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified genomic DNA from each individual was used to prepare paired-end libraries (Lucigen Shotgun library preparation, PCR free) and sequenced on an Illumina HiSeq X PE 150 bp platform to 10-20X coverage at the Centre d’ Expertise et de Services ...
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bioRxiv - Microbiology 2022Quote: ... DNA-Seq library preparation and sequencing were performed by the Genome Quebec Company (Genome Quebec Innovation Center, McGill University, Montreal, Canada) using the Shotgun PCR-free library preparation kit (Lucigen) and the NovaSeq™ 6000 Sequencing system (Illumina® ...
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bioRxiv - Genetics 2021Quote: ... DNA extraction from 2 x 2 x 2mm fin-tissue clips was performed in a 96-well PCR plate using 100μl of QuickExtract solution (Lucigen, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... The extracted samples from passaged cultures were used as a template in a 30-cycle PCR reaction with Econotaq Polymerase (Lucigen) to amplify the leader end of the CRISPR array using primers listed in Table S3 ...
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bioRxiv - Microbiology 2022Quote: ... Clonal populations were genotyped by Sanger sequencing the PCR amplified sgRNA-targeted sites in the gDNA extracted using DNA QuickExtract (Lucigen). Resulting sequences were compared to references and clones containing a frameshift indel or de novo stop codon were selected ...
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bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... or XhoI- (for plasmids with the prefix “pUC57-”) digested plasmids or PCR products using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), followed by capping with ScriptCap m7G Capping System (Cell Script) ...
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bioRxiv - Molecular Biology 2023Quote: ... Single clones were expanded and genotyped for the desired knock-in by PCR on genomic DNA extracted with Quickextract solution (Lucigen). Clones showing biallelic knock-ins were expanded and frozen in LN2 at early passages ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...
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bioRxiv - Biophysics 2023Quote: The templates for preparation of the extended pre-miR-31 for DMS-MaPseq and FL pre-miR-31 for NMR studies were generated by overlap-extension (OE) polymerase chain reaction (PCR) using EconoTaq PLUS 2x Master Mix (Lucigen) with primers listed in Tables S6 and S7 ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Neuroscience 2024Quote: ... Single cell–derived clonal cell lines were analyzed and genotyped by PCR using genomic DNA isolated with QuickExtract DNA Extraction Solution (Lucigen) and primers binding within and downstream the modified region (Primer 1 ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were then used as templates for in vitro transcription per AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies) specifications to obtain dsRNAs.
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bioRxiv - Cell Biology 2020Quote: ... an oligonucleotide containing the Illumina P7 adaptor sequence was ligated to the 3’ end of the single stranded LAM PCR fragments using Circligase (Epicentre/Illumina, #CL9025K). A P5 adaptor was added by PCR ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). The genotyping primers were as described previously29,30,92
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). For embryonic genotyping during primary culture preparation ...
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bioRxiv - Molecular Biology 2019Quote: ... The dsRNAs of target genes were synthesized from the purified PCR products using AmpliScribe T7-Flash Transcription Kit (Epicentre Technologies, Co., Wisconsin, USA).