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Citations for Lucigen :
1 - 50 of 113 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3) Hi-Control (Lucigen) was used as an expression host for heterologous expression of HpuA ...
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bioRxiv - Microbiology 2021Quote: Fosmid libraries of peat lake and freshwater samples were constructed with a pCC2Fos copy control library kit according to manufacturer’s protocol (Epicentre Biotechnologies, Cat. No. CCFOS059), and 198 96-well plates (66 from each sampling location ...
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bioRxiv - Bioengineering 2022Quote: ... genomic DNA was extracted 72 hrs after transfection using QuickExtract (Epicentre). For flow cytometry assays ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic extraction was performed 72 hours post-transfection using QuickExtract (Lucigen). Targeted regions were amplified using Q5 High-Fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA was extracted three days after transfection with QuickExtract (QE) (Lucigen). Cells were washed once with PBS and 50 μL QE was added per well of a 96-well plate ...
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bioRxiv - Microbiology 2019Quote: ... amplified and transformed into Hi-Control 10G cells according to manufactures protocol (Lucigen, Expresso™ T7 cloning and expression system) ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL; Lucigen glass filter and PALL) were also sequenced to reveal contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from cells 72 hours post-transfection with QuickExtract (Lucigen #QE0905) following the manufacturer’s protocol.
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bioRxiv - Genomics 2019Quote: ... We constructed a fosmid library using the Copy Control Fosmid Library Production Kit (Epicentre) and screened it using DIG-labeled (Roche ...
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bioRxiv - Microbiology 2022Quote: ... was resuspended in TE and introduced into BL21(DE3) Hi-Control chemically competent cells (Lucigen), which were selected on LB plates supplemented with kanamycin.
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bioRxiv - Biophysics 2023Quote: ... Genomic DNA was extracted 3 days post-transfection using QuickExtract DNA Extraction Solution 1.0 (Lucigen Corporation QE09050). To test the cutting efficiency ...
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bioRxiv - Neuroscience 2019Quote: ... 20pg of in vitro transcribed RNA synthesized from the T7 control DNA Template (AmpliScribe™ T7 Transcription Kit, Epicentre) was added to the samples ...
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bioRxiv - Cancer Biology 2020Quote: ... HBV plasmid (positive control) and HBV DNA from inoculum was digested with plasmid-safe DNase (Epicentre, E3101K, Madison, WI). According to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: A genomic library of Pseudoalteromonas issachenkonii (isolate no. 91) was constructed using the Copy Control Fosmid Library Production Kit and vector pCC1FOS (Epicentre) with the modifications described by Weiland et al ...
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bioRxiv - Genetics 2020Quote: ... DNA was isolated from tail snips of MyD88 CRISPy TAKO and Mock-treated control offspring using Quick Extract (Lucigen, #QE09050). Primers for MyD88 genotyping are listed in Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... coli (Lucigen, cat. 60242-2) using Bio-Rad MicroPulser Electroporator (cat ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cells (Lucigen, Cat #: EC300110) were thawed on ice for 20 mins ...
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bioRxiv - Immunology 2020Quote: ... coli (Lucigen, Cat# 60502-2) over fifty shocks for each library ...
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bioRxiv - Synthetic Biology 2021Quote: ... Escherichia coli (Epi300, Lucigen, Cat #: EC300110) was grown at 37°C in Luria Broth (LB ...
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bioRxiv - Synthetic Biology 2019Quote: ... Escherichia coli (Epi300, Lucigen, Cat #: EC300110) was grown at 37°C in Luria Broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... Circ Ligase (Epicentre, cat. No. CL4111K), NEBNext® High-Fidelity 2X PCR Master Mix (cat ...
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bioRxiv - Genetics 2020Quote: ... or QuickExtract RNA (Epicentre, Cat# QER090150), respectively ...
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bioRxiv - Systems Biology 2022Quote: ... coli cells (Lucigen cat. no. 60242-1) using prechilled 1 mm electroporation cuvettes (BioRad cat ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10-U endonuclease (Lucigen Cat#DB0715K) for 30-min at 37°C and then incubated at 65°C for 10-min to stop the digestion ...
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bioRxiv - Cancer Biology 2022Quote: ... Endura electrocompetent cells (Lucigen, Cat. No. 60242) were used to perform six electroporation reactions on a MicroPulserTM II (BioRad ...
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bioRxiv - Microbiology 2021Quote: ... and blunt ended ligation (Lucigen, cat#LK0750H) following manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: Baseline-ZERO DNAse (Lucigen, cat. no. DB0715K)
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bioRxiv - Synthetic Biology 2023Quote: ... coli strains Epi300 (Lucigen, Cat #: LGN-EC300110) and MC1061 (NCBI ...
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bioRxiv - Plant Biology 2021Quote: ... coli C41 (DE3) cells (Lucigen, Cat# 60442-1) and purified using Ni-NTA agarose (Qiagen ...
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bioRxiv - Bioengineering 2021Quote: ... coli TG1 (Lucigen, Middleton, WI, Cat#60502-02) to construct two libraries with sizes of 1 × 107 and 2 × 107 independent transformants for Green and Red ...
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bioRxiv - Physiology 2023Quote: ... with RiboGuard RNase inhibitor (Lucigen, Cat # E0126-40D7) and oligo-dT primers (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... and incubated overnight with CircLigaseII (Lucigen cat. CL9025K) and 0.093-0.125 µM biotinylated CL78 primer (31 ...
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bioRxiv - Biochemistry 2021Quote: Kirbac3.1 was expressed in C41(DE3) (Lucigen Cat# 60442) E ...
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bioRxiv - Microbiology 2023Quote: ... for 30 seconds with nylon swabs (Epicentre, cat # QEC89100). Six dental swabs were collected per participant ...
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bioRxiv - Immunology 2023Quote: ... coli strain ClearColi BL21(DE3) (Lucigen Corporation, Cat#60810) at 37 °C for 4 hours with 500 μM IPTG after OD600 reached 0.6-0.8 ...
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bioRxiv - Genomics 2023Quote: ... 1µl Fast-Link DNA ligase (Lucigen, cat. no. LK6201H), 3 µl 10× Fast link ligation buffer and 1mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: QuickExtract DNA Extraction Solution (Lucigen, cat. No. LGN-QE09050)
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bioRxiv - Genetics 2023Quote: ... cloni 10G SUPREME electrocompetent cells (Lucigen Cat#60080-2) using a MicroPulser Electroporator (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... the 3′ ends of small RNA were ligated to an adapter using T4 truncated RNA ligase (Lucigen, Cat# LR2D11310K and NEB, Cat# M0242L), followed by the ligation of the 5′-end adaptors by T4 ligase ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1x reaction buffer (Epicentre cat no. RT80125K and RG90910K). RNA and random decamer primer were first incubated at 85°C for 3 minutes and then incubated on ice for 2 minutes during which remaining reaction components were added ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of Fast-Link DNA ligase (Lucigen, cat#LK0750H), and milliQ water up to 15 μl total volume ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted using the MasterPure kit (Lucigen, cat no MC85200).
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bioRxiv - Cell Biology 2021Quote: ... This sequence was cloned into the pSmart (Lucigen Cat#40041-2) shuttle vector using Gibson assembly (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation reaction was then treated with PlasmidSafe (Epicentre, Cat#: E3101K) to digest any residual linearized DNA ...
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bioRxiv - Genomics 2023Quote: ... 0.2 µl of Fast-Link DNA Ligase (Lucigen, cat. no. LK6201H), 1.2 mM ATP ...
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bioRxiv - Cell Biology 2022Quote: ... and treated with DNase I (cat# D9905K; Lucigen, Middleton, WI, USA). The concentration and purity of the isolated RNA were determined using a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... ssAAV vector samples were incubated with 40-U exonuclease (Lucigen Cat#E3101K) and 10-U endonuclease (Lucigen Cat#DB0715K ...
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bioRxiv - Genomics 2020Quote: To test the Terminator™ 5’-phosphate-dependent exonuclease (Lucigen, Cat. # TER5120), 100 ng of total RNA in 2 μL was combined with 18 μL TEX mix ...
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bioRxiv - Cancer Biology 2019Quote: ... and treated with Plasmid-Safe(tm) ATP-Dependent DNase (Lucigen, cat. E3101K). The reaction was again concentrated by magnetic bead purification and transformed into Lucigen Endura(tm ...