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Citations for Lucigen :
101 - 150 of 441 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... coli BL21(DE3) cells (Lucigen, Middleton, WI) freshly transformed with the βArr1-3A expression plasmid ...
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bioRxiv - Microbiology 2023Quote: ... coli C43 (DE3) electro-competent cells (Lucigen) modified to incorporate a pspA gene knockout60 ...
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bioRxiv - Bioengineering 2024Quote: ... cloni 10G cells were procured from Lucigen. All the primers used in this study were obtained from Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (E. cloni 10G Elite, Lucigen, USA). Further details on the microfluidic screening can be found in the Supplementary Information.
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bioRxiv - Molecular Biology 2021Quote: ... using Endura™ ElectroCompetent Cells (Lucigen 60242-1). Resulting pooled DNA libraries were used to produce lentiviral particles and infect primary cortical neurons.
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bioRxiv - Cell Biology 2019Quote: ... then transformed into Endura ElectroCompetent cells (Lucigen, 60242) according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2019Quote: ... coli 10G chemically competent cells (Lucigen, Middleton, WI). Colonies resistant to kanamycin (Kan ...
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bioRxiv - Genetics 2020Quote: ... cloni 10G SUPREME Electrocompetent Cells (Lucigen, #60080-2), in 11 parallel transformations (1 mL each ...
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bioRxiv - Cell Biology 2021Quote: ... coli EXPRESS BL21(DE3) chemically competent cells (Lucigen). A dense overnight culture was diluted 1:100 into 15 mL LB (Miller ...
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bioRxiv - Plant Biology 2021Quote: ... coli C41 (DE3) cells (Lucigen, Cat# 60442-1) and purified using Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen). All cloning procedures were confirmed by Sanger sequencing using BigDye® Terminator v3.1 Cycle Sequencing kit on 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Biophysics 2019Quote: ... cloni 10G chemically competent cells (Lucigen, Middleton, WI). Colonies resistant to kanamycin (Kan ...
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bioRxiv - Genetics 2019Quote: ... and transformed into electrocompetent cells (Lucigen, Middleton, WI) via a MicroPulser (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... was transformed into electrocompetent Endura cells (#60242, Lucigen) in quadruplicates ...
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bioRxiv - Molecular Biology 2021Quote: Cells were harvested using Quick Extract solution (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... coli SS320 host cells (Lucigen Corporation, Middleton, WI) were electroporated using 30-50 ng of each sub-library DNA in a 0.1 cm cuvette with the following settings ...
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bioRxiv - Genomics 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen 60052-4) in 1mm cuvette (Bio-Rad 1652089 ...
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bioRxiv - Bioengineering 2024Quote: Cells were harvested using Quick Extract solution (Lucigen). Amplicons were generated with Phusion Flash High-Fidelity 2x Mastermix (F548 ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were lysed by QuickExtraction solution (Lucigen) per the manufacturer’s instruction at 65°C for 15 min ...
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bioRxiv - Immunology 2024Quote: ... coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen). All cloning procedures were verified by Sanger sequencing using BigDye® Terminator v3.1 Cycle Sequencing kit on 3500 Genetic Analyzer (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA collected from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), cleaned with RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2020Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, Message BOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2020Quote: Genome DNA of transfected N2a cells or tissue cells were extracted by QuickExtract™ DNA Extraction Solution 1.0 (QE09050, Lucigen, Middleton, WI) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... TOPO plasmids were electroporated into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... The purified product was transformed into electrocompetent cells (Lucigen) via a MicroPulser (Bio-Rad) ...
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bioRxiv - Biophysics 2020Quote: ... cloni EXPRESS BL21 (DE3) cells (Lucigen Corporation, Middleton, USA) were transformed with a pT7 plasmid containing the ClyA-AS gene ...
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bioRxiv - Immunology 2021Quote: ... Recombinant phagmids were electroporated into TG1 cells (Lucigen, USA). A VHH library of 7.3 × 106 individual clones was obtained.
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bioRxiv - Biophysics 2020Quote: ... Cells were lysed using Ready-Lyse Lysozyme (Epicentre, R1802M) followed by 0.5% SDS treatment ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were thoroughly resuspended in 50 µl QuickExtract (Lucigen) and incubated at 65 °C for 20 min and 98 °C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligated product was electroporated into Electrocompetent cells (Lucigen) and grown in liquid culture overnight at 37 degrees before being extracted by Maxiprep ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed using Ready-Lyse Lysozyme (Epicentre, R1802M) in TE for 60 min ...
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bioRxiv - Bioengineering 2023Quote: ... TG1 electrocompetent cells were procured from Lucigen (Middleton, WI). M13KO7 helper phage particle ...
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bioRxiv - Genomics 2023Quote: ... then were transformed into Endura Electrocompetent Cells (Lucigen, 602422) by electroporation (Gene Pulser Xcell Electroporation System ...
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bioRxiv - Genetics 2023Quote: ... The cell pellet was resuspended in Quick-Extract (Lucigen) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The final product was transformed into electrocompetent cells (Lucigen), incubated overnight in LB broth at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The electroporated cells were recovered in recovery medium (Lucigen) for 1 h and then plated on Terrific Broth (TB ...
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bioRxiv - Genetics 2023Quote: ... cloni 10G SUPREME electrocompetent cells (Lucigen Cat#60080-2) using a MicroPulser Electroporator (BioRad) ...
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bioRxiv - Genomics 2020Quote: ... Cells were then lysed with QuickExtract DNA Extraction Solution (Lucigen) and PCR-amplified using primers ...
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bioRxiv - Genomics 2020Quote: ... electroporated in triplicate into Endura electrocompetent cells (Lucigen, Middleton WI), and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Microbiology 2021Quote: ... The vector was transformed into BigEasy-TSA Electrocompetent Cells (Lucigen) for propagation ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was electroporated into Endura competent cells (Lucigen) using 50-100ng DNA per electroporation (we set up 6 or 24 replicate transformation for tiling or genome-wide sgRNA library to maintain library complexity) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the DNA library was transformed into TG1 electrocompetent cells (Lucigen) with an efficiency of approximately 1010 CFU per library before amplification ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed using QuickExtract DNA Extraction Solution (Lucigen) following supplier’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli 10G electrocompetent cells (60080-2, Lucigen, Middleton, WI, US). Cells were selected with appropriate antibiotics on solid and liquid culture ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet resuspended in 100μL QuickExtract solution (Lucigen #QE09050), transferred to PCR-tubes and incubated in a thermocycler for 15 minutes at 68°C ...
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bioRxiv - Bioengineering 2021Quote: ... Each library was independently electroporated into Endura electrocompetent cells (Lucigen), plated on agar bioassay plates ...
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bioRxiv - Genetics 2019Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was extracted from HEP3B cells using QuickExtract (Epicentre).
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bioRxiv - Genomics 2022Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...