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Citations for Lucigen :
1 - 50 of 116 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... including a 1:4 ratio of biotin-16-UTP (BU6105H, Lucigen) to UTP at 5 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.75 mM biotinylated UTP (Biotin-16-UTP, Lucigen BU6105H) and either 7.5 mM GTP or 6.75 mM 7-deazaguanine (TriLink N-1044 ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μg of purified RNA was depleted of ribosomal RNA using a Ribo-Zero rRNA Removal Kit - Plant Leaf (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and in vitro transcription performed using the T7-Flash BiotinRNA Transcription Kit (Epicentre, biotin labelling) or TranscriptAid T7 High Yield Transcription Kit (ThermoScientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 µg RNA was digested with 4 U RNase R (Epicentre) in a total reaction volume of 10 µL for 10 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... purified and then circularized (CircLigase, Lucigen). Amplification with barcoded primers was performed with a few numbers of PCR cycles (5 to 8 ...
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bioRxiv - Microbiology 2021Quote: ... sequences were designed to remove N-terminal signaling peptides and to add a histidine tag for immobilized metal affinity chromatography (IMAC) (in many cases using the Lucigen MA101-Expresso-T7-Cloning-&-Expression-System) ...
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bioRxiv - Microbiology 2020Quote: ... gel purified and circularised using CircLigase (Epicentre) for 1h at 60° C followed by heat inactivation for 10min at 80°C ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Genetics 2019Quote: ... The purified product was transformed into electrocompetent cells (Lucigen) via a MicroPulser (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was end-repaired using End-it from Lucigen. 17.5ul DNA was added to 2.5ul 10X end-it buffer ...
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bioRxiv - Microbiology 2019Quote: ... Total DNA was purified using Masterpure DNA Purification kit (Epicentre), and prepared for TnSeq by PCR amplification of transposon:genome junctions and adapter ligation following the protocol in [53] ...
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bioRxiv - Genetics 2019Quote: ... The extracted RNAs were purified with Rnase R (RNR07250, Epicentre) digestion to remove linear transcripts ...
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bioRxiv - Biochemistry 2021Quote: ... Purified cDNAs were circularized using CircLigase (50 U; CL4111K, Epicentre) for 2 hr at 60 °C and relinearized at the basic dSpacer furan with Ape 1 (15 U ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was electroporated into Endura competent cells (Lucigen) using 50-100ng DNA per electroporation (we set up 6 or 24 replicate transformation for tiling or genome-wide sgRNA library to maintain library complexity) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) and small DNA fragments were preserved by purification with AMPure beads (Agencourt ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified cDNAs were then ligated using CircLigase II (Lucigen) and PCR-amplified to generate the sequencing library.
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bioRxiv - Genetics 2023Quote: ... The purified product was electroporated into Endura competent cells (Lucigen). We extracted the plasmid pool using the Plasmid Maxi prep kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified cDNA was circularized using CircLigase II enzyme (Epicentre, CL9025K), then library PCR ...
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bioRxiv - Genetics 2023Quote: ... 4 units of Ampligase® DNA Ligase (Epicentre), 0.2 ul of Ampligase® 10X Reaction Buffer ...
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bioRxiv - Genomics 2023Quote: ... cloni 10G ELITE Electrocompetent Cells (Lucigen 60052-4) in 1mm cuvette (Bio-Rad 1652089 ...
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bioRxiv - Microbiology 2021Quote: Purified cDNA was subject to circularization via CircLigase ssDNA Ligase (Lucigen) per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2021Quote: ... Purified RNAs were then treated with TAP (Tobacco Acid Pyrophosphatase, Epicentre) to convert 5′ ends of RNA to monophosphate ...
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bioRxiv - Bioengineering 2022Quote: ... the purified RNAs were digested by RNase R exoribonuclease (Lucigen, RNR07520) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al ...
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bioRxiv - Molecular Biology 2021Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified cDNA was then circularized using CircLigaseII ssDNA ligase (Epicentre) for 1[h at 60°C and was subsequently PCR amplified using the primers 5′-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTC-3′ and 5′-CAAGCAGAAGACGGCATACGAGATJJJJJJGTGACTGGAGTTCAGACGTGTG-3′(where Js indicates the 6-mer index sequence for Illumina sequencing).
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bioRxiv - Biochemistry 2023Quote: ... cDNA was column-purified and circularized with CircLigase (Epicentre/Illumina, CL9021K) for 16hrs ...
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bioRxiv - Immunology 2023Quote: ... The ligation reaction was purified and transformed into TG1 bacteria (Lucigen) by electroporation (1.8 kV ...
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bioRxiv - Genomics 2023Quote: ... and gel- purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described36 and libraries constructed using a different reverse indexing primer for each sample.
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bioRxiv - Genomics 2024Quote: ... and lysed using 4 µl Ready-lyse lysozyme (Epicentre) for 20 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... were used to in vitro transcribe a 309-nt actin RNA transcript that was subsequently cap-labeled using recombinant VACV guanylyltransferase/guanine-7-methyltransferase (Epicentre Biotechnologies) in conjunction with capping buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, Cat: CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Immunology 2021Quote: ... and additionally purified with a mRNA-ONLY Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... The genomic DNA was further purified with the MasterPure kit (Lucigen, MC85200) and submitted to sequencing on a HiSeq PE150bp (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Gel-purified cDNAs were then circularized with CircLigase II (Lucigen, Cat: CL4115K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Microbiology 2023Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, cat. CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... purified virus RNA was treated with 5 units of tobacco acid pyrophosphatase (Epicentre) to generate 5’ monophosphorylated termini ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was purified using the MasterPure Gram-positive DNA purification kit (Epicentre) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and additionally purified with a mRNA-ONLY™ Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA was purified using the MasterPure™ Yeast RNA Purification Kit from Epicentre. cDNA was synthesized with random hexamer primers using SuperScript® IV reverse transcriptase ...
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bioRxiv - Genomics 2022Quote: ... Purified ChIP DNA was repaired with End-It DNA End-Repair Kit (Epicentre). The library was then indexed and amplified and sequenced on an Illumina platform ...
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bioRxiv - Genomics 2024Quote: ... The purified library was electroporated in eight replicates into Endura electrocompetent cells (Lucigen), using 50-100 ng/μl DNA and 25 μl cells in 0.1 cm BioRad cuvettes at 1800 V ...
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bioRxiv - Biochemistry 2023Quote: ... The purified total mRNA was treated by 5’-Phosphate-Dependent Exonuclease (Lucigen, TER51020) to degrade RNAs with 5’ monophosphates ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... The size-selected and purified cDNA was circularized using CircLigase ssDNA ligase kit (Lucigen) following manufacture’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli genomic DNA was purified using the MasterPure Gram Positive DNA Purification Kit (Epicentre). All DNAs were purified again using the Zymo Clean and Concentrator-25 (Zymo Research ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). Quantitative PCR (qPCR ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara) ...