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Citations for Lucigen :
151 - 200 of 202 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... coli strains DH5α and C41(DE3) (Lucigen, Middleton, WI) were grown on Luria agar medium at 37 ℃ ...
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bioRxiv - Genomics 2020Quote: ... using a Gibson assembly master mix (New England Biolabs).Gibson assembly products were transformed into electrocompetent cells (E. cloni, Lucigen) and plated on 245mm x 245mm square LB-agar plates to obtain the sufficient number of bacterial colonies at a ∼50× library coverage ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli 10G electrocompetent cells (60080-2, Lucigen, Middleton, WI, US). Cells were selected with appropriate antibiotics on solid and liquid culture ...
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bioRxiv - Genomics 2022Quote: ... Coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).
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bioRxiv - Genomics 2023Quote: ... coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).
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bioRxiv - Biochemistry 2023Quote: ... coli (C41) cells for protein expression were purchased from Lucigen Corporation (Wisconsin) ...
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bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BAC-Optimized Replicator v2.0 (Lucigen; 60210–1), Streptomyces mirabilis P8-A218 ...
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bioRxiv - Biochemistry 2020Quote: ... coli Cloni® 10G cells (Lucigen, USA; 1ul crude reaction mix). Kanamycin selected clones were screened by colony PCR and verified by sequencing ...
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bioRxiv - Microbiology 2019Quote: Escherichia coli S17-130 and EC100D (Epicentre Technologies, Madison, WI, USA) were cultivated at 37°C in LB).
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bioRxiv - Microbiology 2020Quote: Escherichia coli TransforMax Epi300 electrocompetent cells (Ref. EC300110, Epicentre, Madison, WI) and Saccharomyces cerevisiae VL6-48N strain [30] were used to propagate the pCC1BAC-His3 containing viral cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... coli cells (Strand et al., 2014; Lucigen, Middleton, WI, Catalog #EC300110) were transformed via heat shock with sequence-verified pPtPBR1 episomes ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was maintained in 10G elite Escherichia coli bacteria (Lucigen).
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bioRxiv - Genetics 2022Quote: ... single BFU-E and CFU-GM were lysed with QuickExtract Lysis Buffer (Epicentre). CFCs were incubated at 65°C for 20 min followed by an incubation at 98°C for 10 min and centrifuged at 13000 rpm for 10 minutes ...
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bioRxiv - Biophysics 2021Quote: ... coli strain DH5α was isolated using the QuickExtract DNA extraction solution (Lucigen). The coding sequences of the four autoinducer-2 exporter genes were amplified using the Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 Star (DE3) or OverExpress C43 (DE3) (Lucigen/VWR, Radnor, PA). Following induction with IPTG ...
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bioRxiv - Molecular Biology 2023Quote: ... primer sets were screened against a synthetic TSV RNA target in vitro transcribed from gBlock™ DNA (IDT, Table S1) using the Ampliscribe T7-Flash Transcription kit (Lucigen Corporation) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Synthetic Biology 2020Quote: ... All strains were constructed using Escherichia coli strain C43(DE3) (Lucigen, Madison, WI). The deletion of frdABCD ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli genomic DNA in 15 μL of 1× Ampligase DNA Ligase Buffer (Epicentre) containing 250 ng of unshared E ...
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bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli genomic DNA was purified using the MasterPure Gram Positive DNA Purification Kit (Epicentre). All DNAs were purified again using the Zymo Clean and Concentrator-25 (Zymo Research ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10G isa highly competent DH10B derivative [54] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Immunology 2023Quote: The Escherichia coli strains TG1 and AXE688 (21, 22, 23) were purchased from Lucigen Corporation (Middleton ...
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bioRxiv - Cancer Biology 2022Quote: ... The library was transformed into electrocompetent Lucigen Endura™ Escherichia coli (Lucigen; cat. 60242-2) using a Bio-Rad MicroPulser Electroporator (#1652100) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli 10G is a highly competent DH10B derivative [39] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Microbiology 2021Quote: ... The reconstructed product OmRV-fragment1/pACYC177 was then transformed into 10G chemically competent cells (Lucigen, E. cloni), propagated for plasmid purification with the aforementioned method ...
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bioRxiv - Cell Biology 2023Quote: ... and His-SUMO-N was inserted into the backbone of pETite-HisSUMO (Lucigen) using NEBuilder HiFi DNA Assembly Master Mix kit (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: Golden gate assembly reactions were transformed into chemically competent Escherichia coli prepared from strain TG1 (Lucigen). Transformed cells were selected on Lysogeny Broth containing the antibiotics chloramphenicol ...
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bioRxiv - Biophysics 2020Quote: The identified sybody genes of positive clones were chemically-transformed into Escherichia coli MC1061 F- (Lucigen) cells ...
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bioRxiv - Systems Biology 2021Quote: ... We performed nine electroporations of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... according to the manufacturer’s protocol and screened by PCR using a FailSafe™ PCR kit (Buffer E, Epicentre). The presence of MAD1 E53/56K substitutions was identified through PCR using forward primers annealing to the mutated or the wild type sequences (AGCTGGAAAAGAGGGCGAAAC and TAAGTGCCGGGAGATGCTG ...
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bioRxiv - Genomics 2019Quote: ... coli RNA that underwent reverse transcription and amplification using the ScriptSeq Complete Gold Kit for Epidemiology (Epicentre).
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bioRxiv - Genomics 2022Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30°C with shaking at 220 RPM ...
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bioRxiv - Genomics 2023Quote: ... coli colonies were picked into 3-5 mL LB-Kan supplemented with CopyControl Induction Solution (Lucigen CCIS125) and cultured overnight at 30 °C with shaking at 220 RPM ...
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bioRxiv - Genomics 2021Quote: ... We performed 9 electroporations in total of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... coli chromosome was carried out by electroporating 1 μL of the EZ-Tn5
Tnp Transposome (Epicentre) in 50 μL of EV18-pkD46 electrocompetent cells ... -
bioRxiv - Biochemistry 2021Quote: ... coli DnaB and mutants (R74A, R164A, K180A, R328/329A) were independently expressed in C41 strain (Lucigen, Middelton, WI) from pET11b-derived plasmids as previously described [4] ...
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bioRxiv - Molecular Biology 2021Quote: ... The ligation product was purified by isopropanol precipitation and then transformed into electrocompetent Escherichia coli (Lucigen, Middleton, WI, USA). Transformed cells were plated on to 15 cm Luria-Bertani (LB ...
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bioRxiv - Immunology 2023Quote: The extracellular domains of HLA-G2 (HLA-G2) were expressed in Escherichia coli ClearColi BL21(DE3) competent cells (Lucigen) to avoid triggering endotoxic responses in human cells ...
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bioRxiv - Plant Biology 2019Quote: Each ARF DBD was cloned in the pETite N-His SUMO Kan expression vector (Lucigen, WI) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Purified PCR products were ligated into the pETite vector containing an N-terminal His6 tag (Lucigen) and transformed into HI-Control 10G cells ...
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bioRxiv - Microbiology 2021Quote: ... OmRV-fragment2/pMA (ampicillin resistant) and pACYC177 low-copy-number plasmids were transformed into 10G chemically competent cells (Lucigen, E. cloni), respectively ...
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bioRxiv - Neuroscience 2021Quote: ProPeL experiments were carried out as previously described8 with the following conditions for in vivo proteome phosphorylation: all CK2 constructs were expressed in Escherichia coli OverExpress C43(DE3) cells (Lucigen) by IPTG induction ...
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bioRxiv - Biochemistry 2020Quote: ... with Escherichia coli codon usage was synthesized and subcloned into pSol SUMO vector using Expresso® Solubility and Expression Screening System (Lucigen). A pET16b plasmid encoding the fluorescent protein substrate of Mpro (His10-mTurquoise2-TSAVLQSGFRKM-mVenus ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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bioRxiv - Plant Biology 2023Quote: ... a modified PET32a vector with an N-terminal His6-SUMO expression tag and SUMO protease cleavage site (Lucigen Corporation). SALTY fusion was expressed in Codon+ BL21 (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... encoding residues 21 to 344 which lacks the N-terminal mitochondrial-targeting sequence) was cloned into pSol vector (Lucigen) to generate the pSol-His8-SUMO-MGME1 plasmid ...
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bioRxiv - Molecular Biology 2020Quote: Rolling Cycle Amplification (RCA) was done O/N at 30°C using 0.5 Units/μl Φ29 polymerase (Lucigen, 30221-2). The reaction mixture contained also 1X Φ29 buffer ...
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bioRxiv - Neuroscience 2020Quote: DNA from six PVN from each supplemental tactile stimulation group in the repeated room temperature condition and nest temperature condition (total n = 24) was extracted using the Masterpure Complete DNA/RNA Extraction kit (Epicentre) and 300 ng of DNA was used for bisulfite conversion using the Epitect Fast Bisulfite Conversion kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was then incubated at 37 °C for 1 h with 20 U of RNase R (Lucigen, cat n°RNR07250), to remove all the undesired products (i.e linear RNA or concatemer product) ...