Labshake search
Citations for Lucigen :
1 - 50 of 103 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
-
bioRxiv - Molecular Biology 2021Quote: ... were used to in vitro transcribe a 309-nt actin RNA transcript that was subsequently cap-labeled using recombinant VACV guanylyltransferase/guanine-7-methyltransferase (Epicentre Biotechnologies) in conjunction with capping buffer (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: HI-Control™ BL21(DE3) (Lucigen) cells were transformed with plasmid and single colonies were picked to start overnight cultures in LB medium supplemented with 50 μg/mL kanamycin at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3) Hi-Control (Lucigen) was used as an expression host for heterologous expression of HpuA ...
-
bioRxiv - Neuroscience 2020Quote: ... about 15 μg of total RNA of human frontal cortex were treated with 10 units of RNase R (RNR07250, Epicentre) for 1 hour at 37 °C and purified by phenolchloroform extraction ...
-
bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
-
bioRxiv - Biochemistry 2024Quote: ... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
-
bioRxiv - Developmental Biology 2019Quote: ... RNase R (Epicentre) treated for 1 h at 40°C and supplied with DNase I (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNase R (Lucigen) digestion was performed to degrade linear RNA ...
-
bioRxiv - Genetics 2024Quote: Total RNAs were treated with exoribonuclease R (RNase R, Epicentre, RNR07250) to destruct lineRNAs ...
-
bioRxiv - Neuroscience 2022Quote: RNAse R (Lucigen, #RNR07250) treatment was performed on 1 μg total RNA for 10 minutes at 37°C using 5U of enzyme ...
-
bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 18.75 U RNase R (Lucigen), 4.375 U RNase T (NEB) ...
-
bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM KCl ...
-
bioRxiv - Microbiology 2023Quote: ... 20 Units RNase R (Lucigen), 100 mM LiCl ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant phagmids were electroporated into TG1 cells (Lucigen, USA). A VHH library of 7.3 × 106 individual clones was obtained.
-
bioRxiv - Molecular Biology 2020Quote: ... 5 µl RNase R mixture (Epicentre) was added to the sample before incubation at 37°C for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... 20U (1 μl) RNase R (Epicentre) or 1 μl RNase-free water were added to experimental and control samples ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
-
bioRxiv - Microbiology 2019Quote: ... amplified and transformed into Hi-Control 10G cells according to manufactures protocol (Lucigen, Expresso™ T7 cloning and expression system) ...
-
bioRxiv - Cell Biology 2023Quote: ... and His-SUMO-N was inserted into the backbone of pETite-HisSUMO (Lucigen) using NEBuilder HiFi DNA Assembly Master Mix kit (NEB ...
-
bioRxiv - Molecular Biology 2022Quote: ... in 1× RNase R buffer (#RNR07250; Epicentre) in a 10 µl reaction volume at 37 °C for 10 min followed by heat inactivation at 95 °C for 3 min ...
-
bioRxiv - Microbiology 2023Quote: ... and then treated with RNase R (Lucigen). After phenol/chloroform/isopropanol extraction ...
-
bioRxiv - Immunology 2019Quote: The recombinant plasmids were transformed into ClearColi-BL21 (DE3) electrocompetent cells (Lucigen) by heat-shock transformation ...
-
bioRxiv - Developmental Biology 2021Quote: ... In brief, RNA was treated with 20 μl RNase R digestion reaction (2 μg RNA, 1 μl RNase R (Lucigen, #RNR07250), 2 μl 10x RNase R reaction buffer ...
-
bioRxiv - Cell Biology 2021Quote: Total RNAs was treated with RNase R for 30 min at 37°C using 3 U/mg of RNase R (Lucigen, USA). HCC-LM3 and Huh-7 cells treated with actinomycin D (1 µg/mL ...
-
bioRxiv - Microbiology 2022Quote: ... was resuspended in TE and introduced into BL21(DE3) Hi-Control chemically competent cells (Lucigen), which were selected on LB plates supplemented with kanamycin.
-
bioRxiv - Molecular Biology 2020Quote: ... After treatment with RNase R (Epicentre, Madison, WI, USA) to remove the linear RNAs ...
-
bioRxiv - Molecular Biology 2023Quote: Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina) was used to remove ribosomal RNAs and to enrich mRNAs ...
-
bioRxiv - Plant Biology 2019Quote: Each ARF DBD was cloned in the pETite N-His SUMO Kan expression vector (Lucigen, WI) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2019Quote: ... The extracted RNAs were purified with Rnase R (RNR07250, Epicentre) digestion to remove linear transcripts ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA was incubated with 20 U RNase R (Lucigen RNR07250) for 30 mins at 37 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... The CDS encoding FLVA (residues 55-723) was cloned into pETite® C-His Kan Vector (Lucigen) downstream of a sequence for a Strep-tag II (WSHPQFEK ...
-
bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were then introduced into BAC-Optimized Replicator v2.0 electrocompetent cells (60210, Lucigen) via electroporation at 2500V ...
-
bioRxiv - Genetics 2019Quote: ... the rRNA-depleted sample was treated with RNase R (Epicentre, RNR07250) to digest all linear RNAs ...
-
bioRxiv - Genomics 2022Quote: ... rRNA depleted islet RNA was digested using RNase R (RNR07250, Lucigen) or mock digestion (protocol without RNase R ...
-
bioRxiv - Bioengineering 2022Quote: ... the purified RNAs were digested by RNase R exoribonuclease (Lucigen, RNR07520) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... 4 µg RNA was digested with 4 U RNase R (Epicentre) in a total reaction volume of 10 µL for 10 minutes at 37 °C ...
-
bioRxiv - Genomics 2022Quote: ... 5 μg RNA were digested with 1 U RNase R (Epicentre) per μg RNA in a total reaction volume of 10 μl for 10 min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... Linear RNA was subsequently eliminated using RNase R (Epicentre Technologies, USA). The resulting cleaved RNA fragments were reverse-transcribed to generate cDNA ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2µg of total RNA was incubated with 5U RNase R (Lucigen, RNR07250), 10U murine ribonuclease inhibitor (New England Biolabs ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1μg total RNA was incubated with 4U/μg RNase R (#RNR07250, Lucigen) in 10μl reaction system at 37°C for 20min ...
-
bioRxiv - Genetics 2020Quote: ... the linear RNA was digested with 3U of RNase R (Epicentre, USA) per μg of RNA ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 µg RNA was incubated with 5 U RNase R (#RNR07250; Epicentre) in 1× RNase R buffer (#RNR07250 ...
-
bioRxiv - Molecular Biology 2022Quote: ... bromii strain L2-63 and the constructs for Sas6 without the signal peptide were amplified using the primers listed in Table S1 with overhangs complementary to the Expresso T7 Cloning & Expression System N-His pETite vector (Lucigen). The forward primers were engineered to include the 6x His sequence that complemented the vector plus a TEV protease recognition site for later tag removal ...
-
bioRxiv - Microbiology 2022Quote: ... Protein precipitation reagent (Lucigen) was added and samples were spun at maximum speed ...
-
bioRxiv - Developmental Biology 2021Quote: ... the left RNAs were treated with RNase R (Epicentre Inc, Madison, WI, USA) to remove linear RNAs and to enrich circRNAs ...
-
bioRxiv - Plant Biology 2022Quote: ... It was then proceeded using RNase R (3 U/μg; Epicentre, Madison, USA) at 37 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... The RNAs then were subjected to RNase R (Part number: E0111-20D1, Lucigen) treatment following the manufacturer’s instruction in a 20 µl reaction in the presence of lithium chloride containing buffer (Xiao and Wilusz ...
-
bioRxiv - Molecular Biology 2023Quote: ... one 2 microgram portion received 5 units of RNase R (Lucigen, Middleton, WI) and the other an equal volume of nuclease-free water (Thermo Fisher Scientific ...