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Citations for Lucigen :
51 - 100 of 127 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... were sent to BGI (Hongkong) for ribosomal RNA depletion using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) followed by library construction using non-stranded (Replicate 1 ...
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bioRxiv - Immunology 2019Quote: ... The DNase-treated RNA (2 μg) was treated with Ribo-Zero using an Epicentre Ribo-Zero Gold Kit (Human/Rat/Mouse) (Epicentre) and re-purified on Ampure XP beads.
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... the circularization mixture was combined with 6 μl 10X Plasmid-Safe Buffer (Lucigen), 2 μl Plasmid-Safe Enzyme (Lucigen) ...
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bioRxiv - Microbiology 2021Quote: ... fragment 4 was subsequently subcloned into a low-copy plasmid pSMART LCAmp (Lucigen) to increase stability ...
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bioRxiv - Microbiology 2021Quote: ... Linear chromosomal DNA was reduced by Plasmid-Safe ATP-Dependent DNase (Epicentre, USA) treatment for 24 hours at 37°C according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The final plasmid library was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Biochemistry 2022Quote: ... pET15b/MBP-Vpu plasmid was transfected into E.coli BL21(DE3) competent cells (Lucigen) following the recommended by the manufacturer protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was treated with 0.2 units/ul Plasmid-Safe ATP-Dependent DNase (Epicentre) for 5 days at 37 degrees ...
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bioRxiv - Cell Biology 2023Quote: ... and residual linear DNA was degraded by Plasmid-Safe ATP-dependent DNase (Lucigen). In vitro cleavage reactions were performed with 250 ng of Plasmid-Safe-treated circularized DNA ...
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bioRxiv - Biophysics 2023Quote: ... Templates prepared from plasmids were amplified with EconoTaq PLUS 2x Master Mix (Lucigen) using primers UNIV-pUC19_E105 and HDV-AMP-R (Table S8) ...
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bioRxiv - Molecular Biology 2023Quote: Remaining linear DNA molecules were digested with Plasmid-Safe ATP-dependent exonuclease (Epicentre) continuously at 37°C for 120 h ...
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bioRxiv - Systems Biology 2020Quote: ... Purified RNA (2.5 μg) was used for ribosome depletion using a Ribo-Zero™ Gold Kit (Human/Mouse/Rat) (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 5μg of RNA was subjected to ribosomal and mitochondrial RNA depletion using the RiboZero Gold kit (Human/Mouse/Rat, Epicentre #MRZG12324) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: One microgram of total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre, Madison, WI, USA) followed by a purification step using AMPure XP Beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
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bioRxiv - Genomics 2019Quote: ... Remaining linear DNA was removed with Plasmid-Safe-ATP-Dependent DNAse (Epicentre, Madison WI). Guide RNAs were designed using chopchop (http://chopchop.cbu.uib.no/index.php) ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation product was treated with Plasmid-safe nuclease (10,000 units/ml, Epicentre, E3101K) and transformed into SURE2 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... 3) Remaining linear DNA was removed by exonuclease (Plasmid-Safe ATP-dependent DNase, Epicentre), assisted by rare-cutting endonuclease MssI (only support Homo sapiens ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were transformed into E.cloni ® 10G Chemically Competent Cells (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All linearized DNA were removed with 0.4 units Plasmid-Safe™ DNase (Lucigen, E3110K) followed by a 1X SPRI beads purification.
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bioRxiv - Genomics 2021Quote: ... Remaining linear DNA was removed by exonuclease (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,) at 37 °C in a heating block and enzyme reaction was carried out continuously for 1 week ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Molecular Biology 2024Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
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bioRxiv - Bioengineering 2020Quote: ... PP contamination was removed using the Plasmid Safe ATP-dependent DNase kit (Epicentre, WI, USA), and the products where cleaned and concentrated using the Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... were digested overnight with 10 units of Plasmid-Safe ATP-Dependent DNAse (Lucigen, Middleton, Wisconsin) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the assembled plasmid pool was used to transform 25μl of electrocompetent bacteria (Lucigen #60242-2) on a Bio-Rad Gene-Pulser 2 electroporation system (Bio-Rad # 1652105 ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The remaining linear DNA was treated by exonuclease (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K) at 37LJ°C for 1 week ...
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bioRxiv - Genetics 2023Quote: ... 400 ng of DNA was incubated with 5 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Biochemistry 2023Quote: ... The remaining nDNA contamination was removed by treatment with Plasmid-Safe ATP-Dependent DNase (Lucigen) to digest linear DNA53 ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were then introduced into BAC-Optimized Replicator v2.0 electrocompetent cells (60210, Lucigen) via electroporation at 2500V ...
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bioRxiv - Systems Biology 2021Quote: ... We performed nine electroporations of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Three hundred nanograms of purified plasmids were electroporated into 25 µl of Endura electrocompetent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid-safe ATP-dependent DNase treatment was carried out according to the manufacturer’s protocol (Epicenter, Lucigen) with using 1 μg of total DNA extracted from SDGC-SEC-purified stress granule cores and 3 units of the enzyme per reaction ...
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bioRxiv - Bioengineering 2019Quote: ... The plugs are then treated overnight with Plasmid-Safe™ ATP-dependent DNase (50U/ ½ plug) from Epicentre and loaded on a standard gel (1% agarose ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transposed products were enriched by electroporating approximately 100 ng of plasmid pool into Endura Electrocompetent Cells (Lucigen), which are a non- PIR strain that limits recombination ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... linear contaminant DNAs within the crude circular DNA are digested with Plasmid-Safe ATP-dependent DNase (Lucigen) at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The Tn7 integration plasmids with barcode and spectinomycin cassette were extracted from TransforMax EC100D pir + cells (Lucigen) with a Qiagen midi kit ...
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bioRxiv - Genomics 2023Quote: ... Linearized plasmids were used for in vitro transcription with the AmpliScribe T7-Flash Transcription Kit (Lucigen, ASF3507) using 5-Methyluridine-5’-Triphosphate (5-mUTP ...
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bioRxiv - Genomics 2021Quote: ... We performed 9 electroporations in total of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
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bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
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bioRxiv - Immunology 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Genetics 2023Quote: ... 15-20 μg of genomic DNA (gDNA) was digested with ∼95 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Genomics 2024Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...